LOCUS       sequence-257535-        1701 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses beta actin - CIBN - GFP protein fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Salem FB, Bunner WP, Prabhu VV, Kuyateh AB, O'Bryant CT, Murashov 
            AK, Szatmari EM, Hughes RM
  TITLE     CofActor: A light- and stress-gated optogenetic clustering tool to 
            study disease-associated cytoskeletal dynamics in living cells.
  JOURNAL   J Biol Chem. 2020 Aug 7;295(32):11231-11245. doi: 
            10.1074/jbc.RA119.012427. Epub 2020 May 18.
  PUBMED    32424038
REFERENCE   2  (bases 1 to 1701)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA119.012427"; journalName: "J Biol Chem"; date: 
            "2020-08-7- 7"; volume: "295"; issue: "32"; pages: "11231-11245"
COMMENT     Sequence Label: Actin.CIB.GFP
FEATURES             Location/Qualifiers
     source          1..1701
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1156..1665
                     /codon_start=1
                     /product="N-terminal portion of the CIB1 transcription 
                     factor from Arabidopsis thaliana"
                     /label=CIBN
                     /note="binds to CRY2 (cryptochrome 2) that has absorbed 
                     blue light (Kennedy et al., 2010)"
                     /translation="MNGAIGGDLLLNFPDMSVLERQRAHLKYLNPTFDSPLAGFFADSS
                     MITGGEMDSYLSTAGLNLPMMYGETTVEGDSRLSISPETTLGTGNFKAAKFDTETKDCN
                     EAAKKMTMNRDDLVEEGEEEKSKITEQNNGSTKSIKKMKHKAKKEENNFSNDSSKVTKE
                     LEKTDYI"
ORIGIN
        1 atggatgatg atatcgccgc gctcgtcgtc gacaacggct ccggcatgtg caaggccggc
       61 ttcgcgggcg acgatgcccc ccgggccgtc ttcccctcca tcgtggggcg ccccaggcac
      121 cagggcgtga tggtgggcat gggtcagaag gattcctatg tgggcgacga ggcccagagc
      181 aagagaggca tcctcaccct gaagtacccc atcgagcacg gcatcgtcac caactgggac
      241 gacatggaga aaatctggca ccacaccttc tacaatgagc tgcgtgtggc tcccgaggag
      301 caccccgtgc tgctgaccga ggcccccctg aaccccaagg ccaaccgcga gaagatgacc
      361 cagatcatgt ttgagacctt caacacccca gccatgtacg ttgctatcca ggctgtgcta
      421 tccctgtacg cctctggccg taccactggc atcgtgatgg actccggtga cggggtcacc
      481 cacactgtgc ccatctacga ggggtatgcc ctcccccatg ccatcctgcg tctggacctg
      541 gctggccggg acctgactga ctacctcatg aagatcctca ccgagcgcgg ctacagcttc
      601 accaccacgg ccgagcggga aatcgtgcgt gacattaagg agaagctgtg ctacgtcgcc
      661 ctggacttcg agcaagagat ggccacggct gcttccagct cctccctgga gaagagctac
      721 gagctgcctg acggccaggt catcaccatt ggcaatgagc ggttccgctg ccctgaggca
      781 ctcttccagc cttccttcct gggcatggag tcctgtggca tccacgaaac taccttcaac
      841 tccatcatga agtgtgacgt ggacatccgc aaagacctgt acgccaacac agtgctgtct
      901 ggcggcacca ccatgtaccc tggcattgcc gacaggatgc agaaggagat cactgccctg
      961 gcacccagca caatgaagat caagatcatt gctcctcctg agcgcaagta ctccgtgtgg
     1021 atcggcggct ccatcctggc ctcgctgtcc accttccagc agatgtggat cagcaagcag
     1081 gagtatgacg agtccggccc ctccatcgtc caccgcaaat gcttcgatag tgctggtagt
     1141 gctggtagtg ctggtatgaa tggagctata ggaggtgacc ttttgctcaa ttttcctgac
     1201 atgtcggtcc tagagcgcca aagggctcac ctcaagtacc tcaatcccac ctttgattct
     1261 cctctcgccg gcttctttgc cgattcttca atgattaccg gcggcgagat ggacagctat
     1321 ctttcgactg ccggtttgaa tcttccgatg atgtacggtg agacgacggt ggaaggtgat
     1381 tcaagactct caatttcgcc ggaaacgacg cttgggactg gaaatttcaa ggcagcgaag
     1441 tttgatacag agactaagga ttgtaatgag gcggcgaaga agatgacgat gaacagagat
     1501 gacctagtag aagaaggaga agaagagaag tcgaaaataa cagagcaaaa caatgggagc
     1561 acaaaaagca tcaagaagat gaaacacaaa gccaagaaag aagagaacaa tttctctaat
     1621 gattcatcta aagtgacgaa ggaattggag aaaacggatt atattcatgg cggcagcggc
     1681 ggcagccgaa gcttcgaatt c
//
