LOCUS       sequence-257542-        2745 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cry2PHR - mCherry - CofilinS3E protein fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Salem FB, Bunner WP, Prabhu VV, Kuyateh AB, O'Bryant CT, Murashov 
            AK, Szatmari EM, Hughes RM
  TITLE     CofActor: A light- and stress-gated optogenetic clustering tool to 
            study disease-associated cytoskeletal dynamics in living cells.
  JOURNAL   J Biol Chem. 2020 Aug 7;295(32):11231-11245. doi: 
            10.1074/jbc.RA119.012427. Epub 2020 May 18.
  PUBMED    32424038
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.RA119.012427"; journalName: "J Biol Chem"; date: 
            "2020-08-7- 7"; volume: "295"; issue: "32"; pages: "11231-11245"
COMMENT     Sequence Label: Cry2PHR.mCh.CofS3E
FEATURES             Location/Qualifiers
     source          1..2745
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..1494
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     regulatory      1513..1522
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1519..2226
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1664..1682)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1932..1951
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgaagatgg acaaaaagac tatagtttgg tttagaagag acctaaggat tgaggataat
       61 cctgcattag cagcagctgc tcacgaagga tctgtttttc ctgtcttcat ttggtgtcct
      121 gaagaagaag gacagtttta tcctggaaga gcttcaagat ggtggatgaa acaatcactt
      181 gctcacttat ctcaatcctt gaaggctctt ggatctgacc tcactttaat caaaacccac
      241 aacacgattt cagcgatctt ggattgtatc cgcgttaccg gtgctacaaa agtcgtcttt
      301 aaccacctct atgatcctgt ttcgttagtt cgggaccata ccgtaaagga gaagctggtg
      361 gaacgtggga tctctgtgca aagctacaat ggagatctat tgtatgaacc gtgggagata
      421 tactgcgaaa agggcaaacc ttttacgagt ttcaattctt actggaagaa atgcttagat
      481 atgtcgattg aatccgttat gcttcctcct ccttggcggt tgatgccaat aactgcagcg
      541 gctgaagcga tttgggcgtg ttcgattgaa gaactagggc tggagaatga ggccgagaaa
      601 ccgagcaatg cgttgttaac tagagcttgg tctccaggat ggagcaatgc tgataagtta
      661 ctaaatgagt tcatcgagaa gcagttgata gattatgcaa agaacagcaa gaaagttgtt
      721 gggaattcta cttcactact ttctccgtat ctccatttcg gggaaataag cgtcagacac
      781 gttttccagt gtgcccggat gaaacaaatt atatgggcaa gagataagaa cagtgaagga
      841 gaagaaagtg cagatctttt tcttagggga atcggtttaa gagagtattc tcggtatata
      901 tgtttcaact tcccgtttac tcacgagcaa tcgttgttga gtcatcttcg gtttttccct
      961 tgggatgctg atgttgataa gttcaaggcc tggagacaag gcaggaccgg ttatccgttg
     1021 gtggatgccg gaatgagaga gctttgggct accggatgga tgcataacag aataagagtg
     1081 attgtttcaa gctttgctgt gaagtttctt ctccttccat ggaaatgggg aatgaagtat
     1141 ttctgggata cacttttgga tgctgatttg gaatgtgaca tccttggctg gcagtatatc
     1201 tctgggagta tccccgatgg ccacgagctt gatcgcttgg acaatcccgc gttacaaggc
     1261 gccaaatatg acccagaagg tgagtacata aggcaatggc ttcccgagct tgcgagattg
     1321 ccaactgaat ggatccatca tccatgggac gctcctttaa ccgtactcaa agcttctggt
     1381 gtggaactcg gaacaaacta tgcgaaaccc attgtagaca tcgacacagc tcgtgagcta
     1441 ctagctaaag ctatttcaag aacccgtgaa gcacagatca tgatcggagc agcagcccgg
     1501 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     1561 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     1621 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     1681 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     1741 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     1801 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     1861 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     1921 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     1981 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     2041 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     2101 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     2161 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     2221 tacaagggcg gcagcggcgg cggcatggcc gaaggtgtgg ctgtctctga tggtgtcatc
     2281 aaggtgttca atgacatgaa ggttcgcaag tcttcaacac cagaagaagt gaagaaacgc
     2341 aagaaggcgg tgctcttttg cctgagtgag gacaagaaga acatcatcct ggaggagggc
     2401 aaggagatcc tggtaggaga tgtggggcag actgtggacg acccctacac cacttttgtc
     2461 aagatgctgc cagacaagga ctgccgctat gcactctatg atgcaaccta tgagaccaag
     2521 gagagcaaga aggaggacct ggtgttcatc ttctgggccc ccgagaatgc acccctcaag
     2581 agcaaaatga tctatgccag ctccaaggat gccatcaaga agaagctgac aggaatcaag
     2641 catgaattac aagctaactg ctacgaggag gtcaaggacc gctgcaccct ggcagagaaa
     2701 ctaggtggca gcgccgtcat ttccctggag ggcaagcctt tgtga
//
