LOCUS       sequence-257548-        6015 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Omega destination vector, that has a CMV:ELUC:bgH in the backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6015)
  AUTHORS   Sarrion-Perdigones A, Gonzalez Y, Venken KJT
  TITLE     Rapid and Efficient Synthetic Assembly of Multiplex Luciferase 
            Reporter Plasmids for the Simultaneous Monitoring of Up to Six 
            Cellular Signaling Pathways.
  JOURNAL   Curr Protoc Mol Biol. 2020 Jun;131(1):e121. doi: 10.1002/cpmb.121.
  PUBMED    32539183
REFERENCE   2  (bases 1 to 6015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Mol Biol."; date: "2020-06"; volume: "131(1):e121. doi"; pages: " 
            10.1002/cpmb.121"
COMMENT     Sequence Label: Omega Destination-CMV::ELuc:bGH
FEATURES             Location/Qualifiers
     source          1..6015
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    14..238
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(1955..2158)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1984..2004)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2159..2462
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2550..2597
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     rep_origin      complement(3658..4246)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3738..3757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             4714..5505
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      5560..5988
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5647..5666)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5857..5878
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacggatca gcgccataga gcccaccgca tccccagcat gcctgctatt gtcttcccaa
       61 tcctccccct tgctgtcctg ccccacccca ccccccagaa tagaatgaca cctactcaga
      121 caatgcgatg caatttcctc attttattag gaaaggacag tgggagtggc accttccagg
      181 gtcaaggaag gcacggggga ggggcaaaca acagatggct ggcaactaga aggcacagaa
      241 gctcaagccg ccagggcata gttctcatcg ttggcggcgg atgtcttgta catagatcca
      301 ccacccgcct tctccataag tgcttctcgg agttcctttc ggctgatttt gcccgtgacg
      361 ttcctcggaa tagagtcaac gaatctgaca ccccctcgaa gatacttgct gtggctaact
      421 ctttgtgcaa ggaaatcata tacttcttta gccgtcagtt gggccccagg ttgcttgaca
      481 acgaatccgg ccggaagttc ccctgcttct atgtcgggga taccgactac ggccacatcg
      541 cggatccctg gatgttggag caaaatctcc tccaactcga caggagccac ttgatatccc
      601 ttgtacttga tcagctcttt atatctatcc acaatgtaaa aatattcatc ctcatcatag
      661 tagccaaaat caccactgtg aagccaacca tcgtcgtcaa tggcctcctt ggtggcctgt
      721 ggattattca cgtagccttt cgtgaccatg gggccccaaa tgcacaactc tcccacttga
      781 tttgggccaa gcgcctctcc ggtgtttcga tctataattt tagcggccat gtagggcgtc
      841 acttttccca atgaaccgga tttaaattcg ttgtgaagag tgtggatatt agcggatgtg
      901 ctttcagtca acccgtaacc acaccgtatg cctggcaaat taaggcgttt tacggcgatt
      961 tcagctactt cttttgcaag aggtgccgca ccacaacaaa gttccgccaa ggtagaaaga
     1021 tcatacttat caaccaacgg tgatttagaa agaaagagga ttgtacttgg gacattgatc
     1081 acacttcgta cttcatagtc ttgaatcgcc ttcaaaaaaa cctcttggtt gaacctccga
     1141 agcatcacga cgcgcagccc caccataaaa taccccaagt taatggaaaa cccaaaggcg
     1201 tgaaagaagg gaagatacgc cagcacacta actccgggaa tgagttgggt accaactctc
     1261 gggtcagagg cgtgggtcag ccgaacacat atattgcggt gggtctgcat gacgccctta
     1321 ggcaacccgg tagtaccact actacacagt atcgctgcaa cctggtcgac cggatcgtaa
     1381 tgcagtggtt taaaggtctg aagattgtta tcgctatatc ttgacataaa attatgcaag
     1441 gattcacatc cgagaaggtt ttcctcagag tccagaatta tgattctttt gatataattt
     1501 gttctatcct taacctccaa aacctttgga agtatctttc gcgtggtgaa cacaagtatt
     1561 ggcttactaa ttcctgtgac cttacacagt tcgtctggta tatagtcctc gtttacaggt
     1621 gcaacgacca tgcctatata ccacgcacta ataattggaa tgaaaaatct tttgttgttt
     1681 tcagcgcata ttgatacaac atcattcatt ttatagccac aattctggag actctgcccc
     1741 aatttgaccg tcgtgtcgaa gaattcctga taactaagac tttcgttacc catcacgtcg
     1801 aggatcgcct gcgggatgtg actatgtttg tgaagcgcgt tgtagagcat ctctccagcg
     1861 gtaaaattcc caagcgggtg cttaggctcg ggcccataca caacattctt ctctctcttc
     1921 atcatttgag cttgatctga cggttcacta aaccagctct gcttatatag acctcccacc
     1981 gtacacgcct accgcccatt tgcgtcaatg gggcggagtt gttacgacat tttggaaagt
     2041 cccgttgatt ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa
     2101 atccccgtga gtcaaaccgc tatccacgcc cattgatgta ctgccaaaac cgcatcacca
     2161 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
     2221 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
     2281 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
     2341 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
     2401 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
     2461 cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattaataac
     2521 tactccggat cccagggaga gagacgcctg ttgacaatta atcatcggca tagtatatcg
     2581 gcatagtata atacgacaag gtgaggaact aaacccagga ggcagatcaa tggcgagctt
     2641 ggttaagaaa gatatgtgtg ttaagatgac gatggaaggt actgtgaacg gttatcactt
     2701 taagtgcgtt ggcgagggtg aaggcaagcc gttcgagggc acgcagaaca tgcgcattcg
     2761 tgtcaccgag ggcggtccgc tgccttttgc attcgacatc ctggccccgt gctgtatgta
     2821 cggctctaag accttcatta aacacgtgag cggtatcccg gattacttta aagagtcctt
     2881 tccagagggc ttcacttggg aacgtaccca gatttttgag gacggtggtg ttctgaccgc
     2941 gcaccaagac accagcctgg aaggtaattg cctgatctat aaagtgaagg ttctgggtac
     3001 caatttcccg gcgaatggtc cggtgatgca aaagaaaacc gcgggttggg agccgtgcgt
     3061 cgagatgctg tatccgcgtg acggcgtctt gtgtggtcag agcttgatgg cgctgaagtg
     3121 caccgatggc aatcatctga ccagccacct gcgcacgacg tatcgtagcc gtaaaccgag
     3181 caacgccgtt aacatgccgg agttccattt tggtgaccat cgcatcgaaa tcctgaaagc
     3241 tgagcagggc aaattctacg aacaatacga atcggctgtc gcacgttaca gcgatgtgcc
     3301 ggaaaaagcg acgtaatgag cttgaattct gtacactcga gagtctcacc ccaagggcga
     3361 caccccataa ttagcccggg cgaaaggccc agtctttcga ctgagccttt cgttttattt
     3421 gatgcctggc agttccctac tctcgcatgg ggagtcccca cactaccatc ggcgctacgg
     3481 cgtttcactt ctgagttcgg catggggtca ggtgggacca ccgcgctact gccgccaggc
     3541 aaacgtctca cgcttgacac ggttatccac agaatcaggg gataacgcag gaaagaacat
     3601 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     3661 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     3721 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     3781 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     3841 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     3901 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     3961 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4021 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     4081 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     4141 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     4201 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     4261 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     4321 gagattatca aaaaggatct tcacctagat ccttttgtga ccagccagga cagaaatgcc
     4381 tcgacttcgc tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca
     4441 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     4501 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     4561 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     4621 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     4681 cagggcagtc gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta
     4741 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
     4801 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat
     4861 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
     4921 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
     4981 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
     5041 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
     5101 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
     5161 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
     5221 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     5281 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtagctgcc
     5341 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     5401 gcttatcttg gacaagaaga agatcgtttg gcctcgcgcg cagatcagtt ggaagaattt
     5461 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatcact agaccaatgt
     5521 tacacatata tactttagat tgatttaaaa cttcatgtta cgcgccctgt agcggcgcat
     5581 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     5641 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5701 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5761 ccaaaaaact tgattagggt gatggttcac gtagtgggcc agcgccctga tagacggttt
     5821 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     5881 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     5941 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     6001 taacgcttac aattt
//
