LOCUS       sequence-257566-        3837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  c-Myc Renilla luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Sarrion-Perdigones A, Gonzalez Y, Venken KJT
  TITLE     Rapid and Efficient Synthetic Assembly of Multiplex Luciferase 
            Reporter Plasmids for the Simultaneous Monitoring of Up to Six 
            Cellular Signaling Pathways.
  JOURNAL   Curr Protoc Mol Biol. 2020 Jun;131(1):e121. doi: 10.1002/cpmb.121.
  PUBMED    32539183
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Mol Biol."; date: "2020-06"; volume: "131(1):e121. doi"; pages: " 
            10.1002/cpmb.121"
COMMENT     Sequence Label: 7xE-Box::Renilla
FEATURES             Location/Qualifiers
     source          1..3837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    25..73
                     /note="synthetic polyadenylation signal"
     misc_feature    87..178
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     127..146
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        294..325
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    1299..1523
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1647..2235)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1727..1746)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2350..2452
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2453..3268
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2453..3268
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2526..2545)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3382..3810
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3469..3488)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3679..3700
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacggtacc gtctcaaatg ggagaataaa atatctttat tttcattaca tctgtgtgtt
       61 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
      121 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctcg
      181 ctgcatgcgc acgtgtgcac gtggacacgt gtgcgggcac gtggacacgt gctcacgtgc
      241 tcacgtgtcc ctcgaggata tcaaatctgg cctcggcggc caagcttaga cactagaggg
      301 tatataatgg aagctcgact tccagcttgg caatccggta ctgttggtaa agccaccaat
      361 gacttccaag gtttatgacc ccgagcaacg caagagaatg ataactgggc ctcagtggtg
      421 ggccagatgt aaacaaatga atgtccttga tagtttcatc aattactacg acagtgaaaa
      481 gcatgcagaa aacgccgtaa tttttttgca tggcaacgca gcctcctctt atttgtggcg
      541 acacgtagtc ccgcatatag agccggtagc taggtgtatt atccctgatc tgatcggaat
      601 gggaaagagt ggcaagtcag ggaatggatc ttatcgcctt cttgaccact acaagtatct
      661 gacggcttgg ttcgaactct tgaatttgcc gaagaaaatc atcttcgtgg gtcacgattg
      721 gggagcctgt ctcgcgttcc attattccta cgagcatcag gacaagataa aggcaatcgt
      781 tcacgccgag tctgtagtag atgtaatcga gtcctgggac gaatggccag atattgagga
      841 ggatatagca ttgattaaaa gcgaggaagg ggaaaagatg gtgctggaga acaacttctt
      901 tgtggaaaca atgcttcctt caaaaatcat gagaaaactg gaacccgagg aatttgccgc
      961 ttatctcgaa ccgtttaaag aaaagggaga agtccgcagg ccaacacttt cctggccaag
     1021 agaaatcccg ctcgtcaaag gcggaaagcc ggatgttgtg caaatagtcc gaaattacaa
     1081 cgcgtacctc cgggcctctg acgatctccc taaaatgttt atagaatcag atccaggatt
     1141 ttttagcaac gctatcgtag aaggagcaaa gaaatttcca aataccgaat ttgtcaaagt
     1201 aaagggactg catttcagtc aagaggacgc tccagacgag atgggcaaat acataaagtc
     1261 ctttgtagag agggtcctga agaacgaaca gtgagcttct gtgccttcta gttgccagcc
     1321 atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt
     1381 cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct
     1441 ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc
     1501 tggggatgcg gtgggctcta tggcgctgct ttgagacggt acctgacacg gttatccaca
     1561 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     1621 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     1681 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     1741 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     1801 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     1861 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     1921 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     1981 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     2041 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     2101 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     2161 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     2221 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     2281 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     2341 cttttgtgat gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact
     2401 accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatgagcca
     2461 tattcaacgg gaaacgtctt gctcaaggcc gcgattaaat tccaacatgg atgctgattt
     2521 atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa tctaccgatt
     2581 gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa
     2641 tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc ctcttccgac
     2701 catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg cgatcccagg
     2761 gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata ttgttgatgc
     2821 gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc cttttaacag
     2881 cgatcgcgta tttcgactcg ctcaggcgca atcacgaatg aataacggtt tggttgatgc
     2941 gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga aagaaatgca
     3001 taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct cacttgataa
     3061 ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag tcggaatcgc
     3121 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     3181 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     3241 tcatttgatg ctcgatgagt ttttctgatt ttttttaagg cagttattgg tgcccttaaa
     3301 cgcctggttg ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgatga
     3361 taagctgtca aacatgatgt tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     3421 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     3481 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     3541 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     3601 gtgatggttc acgtagtggg ccagcgccct gatagacggt ttttcgccct ttgacgttgg
     3661 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     3721 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     3781 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaattt
//
