LOCUS       sequence-257567-        3869 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Foxo Renilla luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3869)
  AUTHORS   Sarrion-Perdigones A, Gonzalez Y, Venken KJT
  TITLE     Rapid and Efficient Synthetic Assembly of Multiplex Luciferase 
            Reporter Plasmids for the Simultaneous Monitoring of Up to Six 
            Cellular Signaling Pathways.
  JOURNAL   Curr Protoc Mol Biol. 2020 Jun;131(1):e121. doi: 10.1002/cpmb.121.
  PUBMED    32539183
REFERENCE   2  (bases 1 to 3869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Protoc
            Mol Biol."; date: "2020-06"; volume: "131(1):e121. doi"; pages: " 
            10.1002/cpmb.121"
COMMENT     Sequence Label: 3xDBE_RE::Renilla
FEATURES             Location/Qualifiers
     source          1..3869
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    25..73
                     /note="synthetic polyadenylation signal"
     misc_feature    87..178
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     127..146
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     promoter        326..357
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     polyA_signal    1331..1555
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(1679..2267)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1759..1778)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2382..2484
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2485..3300
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             2485..3300
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2558..2577)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3414..3842
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3501..3520)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3711..3732
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 taacggtacc gtctcaaatg ggagaataaa atatctttat tttcattaca tctgtgtgtt
       61 ggttttttgt gtgaatcgat agtactaaca tacgctctcc atcaaaacaa aacgaaacaa
      121 aacaaactag caaaataggc tgtccccagt gcaagtgcag gtgccagaac atttctctcg
      181 ctgaattcga tcaagtaaac aactatgtaa acaagatgca gatcaagtaa acaactatgt
      241 aaacaactcc gttgatcaag taaacaacta tgtaaacaat ccctcgagga tatcaaatct
      301 ggcctcggcg gccaagctta gacactagag ggtatataat ggaagctcga cttccagctt
      361 ggcaatccgg tactgttggt aaagccacca atgacttcca aggtttatga ccccgagcaa
      421 cgcaagagaa tgataactgg gcctcagtgg tgggccagat gtaaacaaat gaatgtcctt
      481 gatagtttca tcaattacta cgacagtgaa aagcatgcag aaaacgccgt aatttttttg
      541 catggcaacg cagcctcctc ttatttgtgg cgacacgtag tcccgcatat agagccggta
      601 gctaggtgta ttatccctga tctgatcgga atgggaaaga gtggcaagtc agggaatgga
      661 tcttatcgcc ttcttgacca ctacaagtat ctgacggctt ggttcgaact cttgaatttg
      721 ccgaagaaaa tcatcttcgt gggtcacgat tggggagcct gtctcgcgtt ccattattcc
      781 tacgagcatc aggacaagat aaaggcaatc gttcacgccg agtctgtagt agatgtaatc
      841 gagtcctggg acgaatggcc agatattgag gaggatatag cattgattaa aagcgaggaa
      901 ggggaaaaga tggtgctgga gaacaacttc tttgtggaaa caatgcttcc ttcaaaaatc
      961 atgagaaaac tggaacccga ggaatttgcc gcttatctcg aaccgtttaa agaaaaggga
     1021 gaagtccgca ggccaacact ttcctggcca agagaaatcc cgctcgtcaa aggcggaaag
     1081 ccggatgttg tgcaaatagt ccgaaattac aacgcgtacc tccgggcctc tgacgatctc
     1141 cctaaaatgt ttatagaatc agatccagga ttttttagca acgctatcgt agaaggagca
     1201 aagaaatttc caaataccga atttgtcaaa gtaaagggac tgcatttcag tcaagaggac
     1261 gctccagacg agatgggcaa atacataaag tcctttgtag agagggtcct gaagaacgaa
     1321 cagtgagctt ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     1381 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     1441 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     1501 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcgctg
     1561 ctttgagacg gtacctgaca cggttatcca cagaatcagg ggataacgca ggaaagaaca
     1621 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     1681 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     1741 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     1801 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     1861 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     1921 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     1981 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     2041 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     2101 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     2161 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     2221 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     2281 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     2341 tgagattatc aaaaaggatc ttcacctaga tccttttgtg atgatcggca cgtaagaggt
     2401 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
     2461 ttttcaggag ctaaggaagc taaaatgagc catattcaac gggaaacgtc ttgctcaagg
     2521 ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat
     2581 gtcgggcaat caggtgcgac aatctaccga ttgtatggga agcccgatgc gccagagttg
     2641 tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta
     2701 aactggctga cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat
     2761 gatgcatggt tactcaccac tgcgatccca gggaaaacag cattccaggt attagaagaa
     2821 tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat
     2881 tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgact cgctcaggcg
     2941 caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc
     3001 tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca
     3061 gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata
     3121 ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta
     3181 tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt
     3241 attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctga
     3301 ttttttttaa ggcagttatt ggtgccctta aacgcctggt tgctacgcct gaataagtga
     3361 taataagcgg atgaatggca gaaattcgat gataagctgt caaacatgat gttacgcgcc
     3421 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     3481 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     3541 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     3601 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccagcgcc
     3661 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     3721 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     3781 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     3841 ttttaacaaa atattaacgc ttacaattt
//
