LOCUS       sequence-257587-       11520 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11520)
  AUTHORS   Sen GL, Webster DE, Barragan DI, Chang HY, Khavari PA
  TITLE     Control of differentiation in a self-renewing mammalian tissue by 
            the histone demethylase JMJD3.
  JOURNAL   Genes Dev. 2008 Jul 15. 22(14):1865-70.
  PUBMED    18628393
REFERENCE   2  (bases 1 to 11520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2008 Jul 15. 22(14):1865-70."
COMMENT     Sequence Label: MSCV JMJD3 mutant
FEATURES             Location/Qualifiers
     source          1..11520
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     12..31
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             231..747
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    811..1152
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1219..1635
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1565..1587
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1603..1619
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1648..1657
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1657..1680
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1693..1719
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1735..1759
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(2180..2191)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2540..2566
                     /codon_start=1
                     /product="9xHis affinity tag"
                     /label=9xHis
                     /translation="HHHHHHHHH"
     CDS             4029..4058
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             4032..4061
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     protein_bind    complement(6852..6876)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6889..7456
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6906..7458
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(7050..7067)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7277..7296
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7470..8069
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7470..7489)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7966..7986
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             8145..8659
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    8821..8837
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8845..8851,8852..8869,8870..8875))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8890..8911
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9028..9045)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9199..9787)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9279..9298)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9958..10749,10750..10818))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10581..10600
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10819..10923)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10991..11009
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11047..11069)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11169..11188
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11382..11404
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
       61 atgccggcca cgatgcgtcc ggcgtagagg cgattagtcc aatttgttaa agacaggata
      121 tcagtggtcc aggctctagt tttgactcaa caatatcacc agctgaagcc tatagagtac
      181 gagccataga taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac
      241 cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaata
      301 cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag cagaatatgg
      361 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
      421 tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt ttccagggtg
      481 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
      541 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg
      601 gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtattcccaa taaagcctct
      661 tgctgtttgc atccgaatcg tggactcgct gatccttggg agggtctcct cagattgatt
      721 gactgcccac ctcgggggtc tttcatttgg aggttccacc gagatttgga gacccctgcc
      781 cagggaccac cgaccccccc gccgggaggt aagctggcca gcggtcgttt cgtgtctgtc
      841 tctgtctttg tgcgtgtttg tgccggcatc taatgtttgc gcctgcgtct gtactagtta
      901 gctaactagc tctgtatctg gcggacccgt ggtggaactg acgagttctg aacacccggc
      961 cgcaaccctg ggagacgtcc cagggacttt gggggccgtt tttgtggccc gacctgagga
     1021 agggagtcga tgtggaatcc gaccccgtca ggatatgtgg ttctggtagg agacgagaac
     1081 ctaaaacagt tcccgcctcc gtctgaattt ttgctttcgg tttggaaccg aagccgcgcg
     1141 tcttgtctgc tgcagcgctg cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg
     1201 tatttgtctg aaaattaggg ccagactgtt accactccct taagtttgac cttaggtcac
     1261 tggaaagatg tcgagcggat cgctcacaac cagtcggtag atgtcaagaa gagacgttgg
     1321 gttaccttct gctctgcaga atggccaacc tttaacgtcg gatggccgcg agacggcacc
     1381 tttaaccgag acctcatcac ccaggttaag atcaaggtct tttcacctgg cccgcatgga
     1441 cacccagacc aggtccccta catcgtgacc tgggaagcct tggcttttga cccccctccc
     1501 tgggtcaagc cctttgtaca ccctaagcct ccgcctcctc ttcctccatc cgccccgtct
     1561 ctcccccttg aacctcctcg ttcgaccccg cctcgatcct ccctttatcc agccctcact
     1621 ccttctctag gcgccggaat tagatctgcc accatggact acaaggatga cgatgacaag
     1681 ctcgatggag gatacccata cgatgttcca gattacgctg ctcgaggtta tcaaacaagt
     1741 ttgtacaaaa aagcaggctc cgcggccgcc cccttcaccg gatccatgca tcgggcagtg
     1801 gatcctccag gggcccgcgc tgcacgggaa gcctttgccc ttgggggcct gagctgtgct
     1861 ggggcctgga gctcctgccc gcctcatccc cctcctcgta gcgcatggct gcctggaggc
     1921 agatgctcag ccagcattgg gcagcccccg cttcctgctc ccctaccccc ttcacatggc
     1981 agtagttctg ggcaccccag caaaccatat tatgctccag gggcgcccac tccaagaccc
     2041 ctccatggga agctggaatc cctgcatggc tgtgtgcagg cattgctccg ggagccagcc
     2101 cagccagggc tttgggaaca gcttgggcaa ctgtacgagt cagagcacga tagtgaggag
     2161 gccacacgct gctaccacag cgcccttcga tacggaggaa gcttcgctga gctggggccc
     2221 cgcattggcc gactgcagca ggcccagctc tggaactttc atactggctc ctgccagcac
     2281 cgagccaagg tcctgccccc actggagcaa gtgtggaact tgctacacct tgagcacaaa
     2341 cggaactatg gagccaagcg gggaggtccc ccggtgaagc gagctgctga acccccagtg
     2401 gtgcagcctg tgcctcctgc agcactctca ggcccctcag gggaggaggg cctcagccct
     2461 ggaggcaagc gaaggagagg ctgcaactct gaacagactg gccttccccc agggctgcca
     2521 ctgcctccac caccattacc accaccacca ccaccaccac caccacccct gcctggcctg
     2581 gctaccagcc ccccatttca gctaaccaag ccagggctgt ggagtaccct gcatggagat
     2641 gcctggggcc cagagcgcaa gggttcagca cccccagagc gccaggagca gcggcactcg
     2701 ctgcctcacc catatccata cccagctcca gcgtacaccg cgcacccccc tggccaccgg
     2761 ctggtcccgg ctgctccccc aggcccaggc ccccgccccc caggagcaga gagccatggc
     2821 tgcctgcctg ccacccgtcc ccccggaagt gaccttagag agagcagagt tcagaggtcg
     2881 cggatggact ccagcgtttc accagcagca accaccgcct gcgtgcctta cgccccttcc
     2941 cggccccctg gcctccccgg caccaccacc agcagcagca gtagcagcag cagcaacact
     3001 ggtctccggg gcgtggagcc gaacccaggc attcccggcg ctgaccatta ccaaactccc
     3061 gcgctggagg tctctcacca tggccgcctg gggccctcgg cacacagcag tcggaaaccg
     3121 ttcttggggg ctcccgctgc cactccccac ctatccctgc cacctggacc ttcctcaccc
     3181 cctccacccc cctgtccccg cctcttacgc cccccaccac cccctgcctg gttgaagggt
     3241 ccggcctgcc gggcagcccg agaggatgga gagatcttag aagagctctt ctttgggact
     3301 gagggacccc cccgccctgc cccaccaccc ctcccccatc gcgagggctt cttggggcct
     3361 ccggcctccc gcttttctgt gggcactcag gattctcaca cccctcccac tcccccaacc
     3421 ccaaccacca gcagtagcaa cagcaacagt ggcagccaca gcagcagccc tgctgggcct
     3481 gtgtcctttc ccccaccacc ctatctggcc agaagtatag acccccttcc ccggcctccc
     3541 agcccagcac agaaccccca ggacccacct cttgtacccc tgactcttgc cctgcctcca
     3601 gcccctcctt cctcctgcca ccaaaatacc tcaggaagct tcaggcgccc ggagagcccc
     3661 cggcccaggg tctccttccc aaagaccccc gaggtggggc cggggccacc cccaggcccc
     3721 ctgagtaaag ccccccagcc tgtgccgccc ggggttgggg agctgcctgc ccgaggccct
     3781 cgactctttg attttccccc cactccgctg gaggaccagt ttgaggagcc agccgaattc
     3841 aagatcctac ctgatgggct ggccaacatc atgaagatgc tggacgaatc cattcgcaag
     3901 gaagaggaac agcaacaaca cgaagcaggc gtggcccccc aacccccgct gaaggagccc
     3961 tttgcatctc tgcagtctcc tttccccacc gacacagccc ccaccactac tgctcctgct
     4021 gtcgccgtca ccaccaccac caccaccacc accaccacca cggccaccca ggaagaggag
     4081 aagaagccac caccagccct accaccacca ccgcctctag ccaagttccc tccaccctct
     4141 cagccacagc caccaccacc cccacccccc agcccggcca gcctgctcaa atccttggcc
     4201 tccgtgctgg agggacaaaa gtactgttat cgggggactg gagcagctgt ttccacccgg
     4261 cctgggccct tgcccaccac tcagtattcc cctggccccc catcaggtgc taccgccctg
     4321 ccgcccacct cagcggcccc tagcgcccag ggctccccac agccctctgc ttcctcgtca
     4381 tctcagttct ctacctcagg cgggccctgg gcccgggagc gcagggcggg cgaagagcca
     4441 gtcccgggcc ccatgacccc cacccaaccg cccccacccc tatctctgcc ccctgctcgc
     4501 tctgagtctg aggtgctaga agagatcagc cgggcttgcg agacccttgt ggagcgggtg
     4561 ggccggagtg ccactgaccc agccgaccca gtggacacag cagagccagc ggacagtggg
     4621 actgagcgac tgctgccccc cgcacaggcc aaggaggagg ctggcggggt ggcggcagtg
     4681 tcaggcagct gtaagcggcg acagaaggag catcagaagg agcatcggcg gcacaggcgg
     4741 gcctgtaagg acagtgtggg tcgtcggccc cgtgagggca gggcaaaggc caaggccaag
     4801 gtccccaaag aaaagagccg ccgggtgctg gggaacctgg acctgcagag cgaggagatc
     4861 cagggtcgtg agaagtcccg gcccgatctt ggcggggcct ccaaggccaa gccacccaca
     4921 gctccagccc ctccatcagc tcctgcacct tctgcccagc ccacaccccc gtcagcctct
     4981 gtccctggaa agaaggctcg ggaggaagcc ccagggccac cgggtgtcag ccgggccgac
     5041 atgctgaagc tgcgctcact tagtgagggg ccccccaagg agctgaagat ccggctcatc
     5101 aaggtagaga gtggtgacaa ggagaccttt atcgcctctg aggtggaaga gcggcggctg
     5161 cgcatggcag acctcaccat cagccactgt gctgctgacg tcgtgcgcgc cagcaggaat
     5221 gccaaggtga aagggaagtt tcgagagtcc tacctttccc ctgcccagtc tgtgaaaccg
     5281 aagatcaaca ctgaggagaa gctgccccgg gaaaaactca acccccctac acccagcatc
     5341 tatctggaga gcaaacggga tgccttctca cctgtcctgc tgcagttctg tacagaccct
     5401 cgaaatccca tcacagtgat ccggggcctg gcgggctccc tgcggctcaa cttgggcctc
     5461 ttctccacca agaccctggt ggaagcgagt ggcgaacaca ccgtggaagt tcgcacccag
     5521 gtgcagcagc cctcagatga gaactgggat ctgacaggca ctcggcagat ctggccttgt
     5581 gagagctccc gttcccacac caccattgcc aagtacgcac agtaccaggc ctcatccttc
     5641 caggagtctc tgcaggagga gaaggagagt gaggatgagg agtcagagga gccagacagc
     5701 accactggaa cccctcctag cagcgcacca gacccgaaga accatcacat catcaagttt
     5761 ggcaccaaca tcgacttgtc tgatgctaag cggtggaagc cccagctgca ggagctgcta
     5821 aagctgcccg ccttcatgcg ggtaacatcc acgggcaaca tgctgagcca cgtgggccac
     5881 accatcctgg gcatgaacac ggtgcagctg tacatgaagg tgcccggcag ccgaacgcca
     5941 ggcgctcagg agaataacaa cttctgctcc gtcaacatca acattggccc aggcgactgc
     6001 gagtggttcg cggtgcacga gcactactgg gagaccatca gcgctttctg tgatcggcac
     6061 ggcgtggact acttgacggg ttcctggtgg ccaatcctgg atgatctcta tgcatccaat
     6121 attcctgtgt accgcttcgt gcagcgaccc ggagacctcg tgtggattaa tgcggggact
     6181 gtgcactggg tgcaggccac cggctggtgc aacaacattg cctggaacgt ggggcccctc
     6241 accgcctatc agtaccagct ggccctggaa cgatacgagt ggaatgaggt gaagaacgtc
     6301 aaatccatcg tgcccatgat tcacgtgtca tggaacgtgg ctcgcacggt caaaatcagc
     6361 gaccccgact tgttcaagat gatcaagttc tgcctgctgc agtccatgaa gcactgccag
     6421 gtgcaacgcg agagcctggt gcgggcaggg aagaaaatcg cttaccaggg ccgtgtcaag
     6481 gacgagccag cctactactg caacgagtgc gatgtggagg tgtttaacat cctgttcgtg
     6541 acaagtgaga atggcagccg caacacgtac ctggtacact gcgagggctg tgcccggcgc
     6601 cgcagcgcag gcctgcaggg cgtggtggtg ctggagcagt accgcactga ggagctggct
     6661 caggcctacg acgccttcac gctggtgagg gcccggcggg cgcgcgggca gcggaggagg
     6721 gcactggggc aggctgcagg gacgggcttc gggagcccgg ccgcgccttt ccctgagccc
     6781 ccgccggctt tctcccccca ggccccagcc agcacgtcgc gatgactcga ggcgaaaggg
     6841 tgggcgcgcc gacccagctt tcttgtacaa agtggttcga taacgaattc cgcccccccc
     6901 ccctaacgtt actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt
     6961 attttccacc atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt
     7021 cttgacgagc attcctaggg gtctttcccc tctcgccaaa ggaatgcaag gtctgttgaa
     7081 tgtcgtgaag gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac
     7141 cctttgcagg cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg
     7201 tgtataagat acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt
     7261 tgtggaaaga gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca
     7321 gaaggtaccc cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt
     7381 ttagtcgagg ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg
     7441 aaaaacacga tgataatatg gccacaacca tgaccgagta caagcccacg gtgcgcctcg
     7501 ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc
     7561 ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag
     7621 aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg
     7681 ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga
     7741 tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag
     7801 gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct
     7861 cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg
     7921 ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct
     7981 acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct
     8041 ggtgcatgac ccgcaagccc ggtgcctgat gcatcgatgt cgacctgcag ccaagcttat
     8101 cgataaaata aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct
     8161 gtaggtttgg caagctagct taagtaacgc cattttgcaa ggcatggaaa atacataact
     8221 gagaatagag aagttcagat caaggttagg aacagagaga cagcagaata tgggccaaac
     8281 aggatatctg tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggtccccag
     8341 atgcggtccc gccctcagca gtttctagag aaccatcaga tgtttccagg gtgccccaag
     8401 gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt
     8461 tcgcgcgctt ctgctccccg agctcaataa aagagcccac aacccctcac tcggcgcgcc
     8521 agtcctccga tagactgcgt cgcccgggta cccgtgtatc caataaaccc tcttgcagtt
     8581 gcatccgact tgtggtctcg ctgttccttg ggagggtctc ctctgagtga ttgactaccc
     8641 gtcagcgggg gtctttcatg ggtaacagtt tcttgaagtt ggagaacaac attctgaggg
     8701 taggagtcga atattaagta atcctgactc aattagccac tgttttgaat ccacatactc
     8761 caatactcct gaaatagttc attatggaca gcgcagaaag agctggggag aattgtgaaa
     8821 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8881 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8941 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     9001 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     9061 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     9121 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     9181 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     9241 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     9301 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     9361 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     9421 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     9481 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9541 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9601 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     9661 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9721 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9781 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9841 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9901 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9961 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
    10021 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
    10081 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
    10141 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
    10201 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
    10261 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
    10321 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
    10381 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
    10441 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
    10501 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
    10561 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
    10621 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
    10681 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
    10741 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
    10801 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
    10861 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
    10921 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
    10981 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
    11041 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
    11101 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
    11161 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
    11221 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
    11281 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
    11341 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
    11401 aacgacggcg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg
    11461 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
//
