LOCUS       sequence-257596-        8909 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  extGluc luciferase for invivo Imaging, mKate and NGFR for 
            flourescent detection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Ebinger S, Ozdemir EZ, Ziegenhain C, Tiedt S, Castro Alves C, 
            Grunert M, Dworzak M, Lutz C, Turati VA, Enver T, Horny HP, Sotlar 
            K, Parekh S, Spiekermann K, Hiddemann W, Schepers A, Polzer B, 
            Kirsch S, Hoffmann M, Knapp B, Hasenauer J, Pfeifer H, 
            Panzer-Grumayer R, Enard W, Gires O, Jeremias I
  TITLE     Characterization of Rare, Dormant, and Therapy-Resistant Cells in 
            Acute Lymphoblastic Leukemia.
  JOURNAL   Cancer Cell. 2016 Dec 12;30(6):849-862. doi: 
            10.1016/j.ccell.2016.11.002. Epub 2016 Dec 1.
  PUBMED    27916615
REFERENCE   2  (bases 1 to 8909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ccell.2016.11.002"; journalName: "Cancer Cell"; date: 
            "2016-12-12- 12"; volume: "30"; issue: "6"; pages: "849-862"
COMMENT     Sequence Label: pCDH-EF1a-extGluc-mKate-NGFR
FEATURES             Location/Qualifiers
     source          1..8909
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    266..382
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        425..636
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             649..917
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     regulatory      957..966
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1776..1829
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1836..2531
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             2538..2591
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     misc_feature    3447..4035
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3500..3520)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3918..3929)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4109..4342
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4414..4535
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4451..4470)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4505..4524
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4575..4710
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4743..4759)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4743..4759)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4756..4778)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4767..4783
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4791..4797,4798..4815,4816..4821))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4836..4857
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4974..4991)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5145..5733)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(5904..6695,6696..6764))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6527..6546
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6765..6869)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6937..6955
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6993..7015)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     7115..7134
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7328..7350
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7342..7359
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7343..7359
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7374..7600
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7601..7781
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7828..7953
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8446..8679
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8864..8908
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
       61 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
      121 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
      181 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
      241 acggatctcg acggtatcgg ttaactttta aaagaaaagg ggggattggg gggtacagtg
      301 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
      361 aaattacaaa attcaaaatt ttatcgatac tagtggatct gcgatcgctc cggtgcccgt
      421 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat
      481 tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
      541 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
      601 gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc
      661 tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt
      721 ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag
      781 ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg
      841 ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt
      901 ctgcgccgtt acagatccaa gctgtgaccg gcgcctactc tagagctagc gaattcgcca
      961 ccatggctct cccagtgact gccctactgc ttcccctagc gcttctcctg catgccaagc
     1021 ccaccgagaa caacgaagac ttcaacatcg tggccgtggc cagcaacttc gcgaccacgg
     1081 atctcgatgc tgaccgcggg aagttgcccg gcaagaagct gccgctggag gtgctcaaag
     1141 agatggaagc caatgcccgg aaagctggct gcaccagggg ctgtctgatc tgcctgtccc
     1201 acatcaagtg cacgcccaag atgaagaagt tcatcccagg acgctgccac acctacgaag
     1261 gcgacaaaga gtccgcacag ggcggcatag gcgaggcgat cgtggacatt cctgagattc
     1321 ctgggttcaa ggacttggag ccaatggagc agttcatcgc acaggtcgat ctgtgtgtgg
     1381 actgcacaac tggctgcctc aaagggcttg ccaacgtgca gtgttctgac ctgctcaaga
     1441 agtggctgcc gcaacgctgt gcgacctttg ccagcaagat ccagggccag gtggacaaga
     1501 tcaagggggc cggtggtgac ttagcggccg cacccaccac gacgccagcg ccgcgaccac
     1561 caaccccggc gcccacgatc gcgtcgcagc ccctatccct gcgcccagag gcgtgccggc
     1621 cagcggcggg gggcgcagtg cacacgaggg ggctggactt cgcctgtgat atctacatct
     1681 gggcgcccct ggccgggact tgtggggtcc ttctcctgtc actggttatc accctttact
     1741 gcaaccacag gcggattcaa ggatccgcgg ccgctgaggg cagaggaagt cttctaacat
     1801 gcggtgacgt ggaggagaat cccggcccta tgcatatggt gagcgagctg attaaggaga
     1861 acatgcacat gaagctgtac atggagggca ccgtgaacaa ccaccacttc aagtgcacat
     1921 ccgagggcga aggcaagccc tacgagggca cccagaccat gagaatcaag gcggtcgagg
     1981 gcggccctct ccccttcgcc ttcgacatcc tggctaccag cttcatgtac ggcagcaaaa
     2041 ccttcatcaa ccacacccag ggcatccccg acttctttaa gcagtccttc cccgagggct
     2101 tcacatggga gagagtcacc acatacgaag acgggggcgt gctgaccgct acccaggaca
     2161 ccagcctcca ggacggctgc ctcatctaca acgtcaagat cagaggggtg aacttcccat
     2221 ccaacggccc tgtgatgcag aagaaaacac tcggctggga ggcctccacc gagaccctgt
     2281 accccgctga cggcggcctg gaaggcagag ccgacatggc cctgaagctc gtgggcgggg
     2341 gccacctgat ctgcaacttg aagaccacat acagatccaa gaaacccgct aagaacctca
     2401 agatgcccgg cgtctactat gtggacagaa gactggaaag aatcaaggag gccgacaaag
     2461 agacctacgt cgagcagcac gaggtggctg tggccagata ctgcgacctc cctagcaaac
     2521 tggggcacag actcgaggag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga
     2581 atcccggccc ttccggaatg ggggcaggtg ccaccggccg cgccatggac gggccgcgcc
     2641 tgctgctgtt gctgcttctg ggggtgtccc ttggaggtgc caaggaggca tgccccacag
     2701 gcctgtacac acacagcggt gagtgctgca aagcctgcaa cctgggcgag ggtgtggccc
     2761 agccttgtgg agccaaccag accgtgtgtg agccctgcct ggacagcgtg acgttctccg
     2821 acgtggtgag cgcgaccgag ccgtgcaagc cgtgcaccga gtgcgtgggg ctccagagca
     2881 tgtcggcgcc gtgcgtggag gccgacgacg ccgtgtgccg ctgcgcctac ggctactacc
     2941 aggatgagac gactgggcgc tgcgaggcgt gccgcgtgtg cgaggcgggc tcgggcctcg
     3001 tgttctcctg ccaggacaag cagaacaccg tgtgcgagga gtgccccgac ggcacgtatt
     3061 ccgacgaggc caaccacgtg gacccgtgcc tgccctgcac cgtgtgcgag gacaccgagc
     3121 gccagctccg cgagtgcaca cgctgggccg acgccgagtg cgaggagatc cctggccgtt
     3181 ggattacacg gtccacaccc ccagagggct cggacagcac agcccccagc acccaggagc
     3241 ctgaggcacc tccagaacaa gacctcatag ccagcacggt ggcaggtgtg gtgaccacag
     3301 tgatgggcag ctcccagccc gtggtgaccc gaggcaccac cgacaacctc atccctgtct
     3361 attgctccat cctggctgct gtggttgtgg gccttgtggc ctacatagcc ttcaagaggt
     3421 ggaactgaag ctgaatctaa gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     3481 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     3541 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     3601 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     3661 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     3721 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     3781 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     3841 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     3901 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     3961 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     4021 gggccgcctc cccgcctggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     4081 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagataa
     4141 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     4201 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     4261 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     4321 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     4381 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     4441 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     4501 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     4561 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     4621 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     4681 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagaccaa attcgtaatc
     4741 atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga
     4801 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt
     4861 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga
     4921 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     4981 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     5041 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     5101 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     5161 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     5221 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     5281 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     5341 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     5401 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     5461 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5521 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5581 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5641 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     5701 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     5761 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     5821 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     5881 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     5941 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     6001 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     6061 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     6121 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     6181 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     6241 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     6301 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     6361 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     6421 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6481 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     6541 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     6601 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     6661 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     6721 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     6781 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     6841 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
     6901 taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt
     6961 cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     7021 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     7081 ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga
     7141 gtgcaccata tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg
     7201 cgccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg
     7261 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca
     7321 gggttttccc agtcacgacg ttgtaaaacg acggccagtg ccaagctgac gcgtgtagtc
     7381 ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca
     7441 aggagagaaa aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt
     7501 attaggaagg caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg
     7561 cagagatatt gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca
     7621 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7681 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7741 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
     7801 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
     7861 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
     7921 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
     7981 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
     8041 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
     8101 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
     8161 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
     8221 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
     8281 gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg acaattggag
     8341 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     8401 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     8461 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     8521 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     8581 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     8641 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     8701 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     8761 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     8821 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
     8881 attggaatta gataaatggg caagtttgt
//
