LOCUS       sequence-257603-        5278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway (Invitrogen) promoter clone (pDONR_P4-P1R) with premature 
            stop codons introduced within the Xero1 and Xero2 genes in the PER36
            promoter entry clone (Kunieda et al. 2013)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   McGee R, Dean GH, Mansfield SD, Haughn GW
  TITLE     Assessing the utility of seed coat-specific promoters to engineer 
            cell wall polysaccharide composition of mucilage.
  JOURNAL   Plant Mol Biol. 2019 Aug 17. pii: 10.1007/s11103-019-00909-8. doi: 
            10.1007/s11103-019-00909-8.
  PUBMED    31422517
REFERENCE   2  (bases 1 to 5278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol 
            Biol. 2019 Aug 17. pii: 10.1007/s11103-019-00909-8. doi: 
            10.1007/s11103-019-00909-8."
COMMENT     Sequence Label: pDONR_P4-P1R:PER36modp
FEATURES             Location/Qualifiers
     source          1..5278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    3323..3447
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3519..3538)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3520..3538)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3543..3559)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3543..3559)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3661..3680)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3672..4481
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3739..3758)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4628..5216
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5117..5136
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttggg cccatataag ttaaaattac gtcggtcgct
      721 aaccactact acaccgacgt cttacttgcc atgtgtgtgt gactcttaat caattacgaa
      781 ttgaatatat tacttttacg atgtcggcca acacgtattg agtaaaatat ctatgtgatg
      841 atgaattcct atccaaaatg aaatttgcat ctctataaaa gtatcattca agagatcatt
      901 aatcttcatc gatcaaagtc ttgaatattt catctatata tacctaagaa agaaaagagt
      961 taaagaagta tattttcgat tcaaagaaga aaaatgaatt ctcaccagaa tcaaaccgga
     1021 gtgcaaaaga agtgaataac ggagaaaatc atggagaagc taccaggtca tcatggacct
     1081 actaacactg gtgttgttca ccacgagaag aaagggatga cggagaaagt tatggagcag
     1141 ctgccaggtc atcatggtgc tactggtacc ggtggtgttc accacgagaa gaaaggaatg
     1201 acggaaaaag ttatggagca gctgccaggt catcatggat cacaccaaac tggaaccaac
     1261 acaacttatg gtactactaa cactggtggt gttcaccacg agaaaaaaag tgtgaccgaa
     1321 aaagttatgg agaaactacc aggtcatcat gggtcacatc aaactgggac taacacggct
     1381 tatggtacta acactaatgt cgttcaccat gagaaaaaag gtatagcaga aaagattaaa
     1441 gagcaactgc caggtcatca tggaactcac aaaactggaa ccaccacaag ttatggtaat
     1501 actggcgttg ttcaccatga aaataaaagt acgatggata agattaaaga gaagcttccc
     1561 ggtggtcatc actagattga ttaacaactt gtttaagctt attttaagta tttgtgtggt
     1621 gtaataatat gatgatgtgg ctttctttca ttgagtcgct aaataatata tgtaagtgat
     1681 ggatctttga attaatataa aatatctact tctggtttcg tttatttatc tttctatcgc
     1741 ttcaactatt ccatgttatt aaaatacttg tgagttgtga cacatacaac cacgtataat
     1801 tcacatgtaa ttatttggta attcaattct ttacaagttt atatgttctt ttgcatacaa
     1861 gttatcaggg aaacgaccgc agttgtttgg caatgtatat tactcaggtt ttcaattcag
     1921 gcccatacgg cccaaataag ttgtttttac ggatatcgag ttaaaagagg tgtgtcgatt
     1981 ataacggaca tgtgtcacac taaccacaaa accaacgtct tacgtaatcg ccacgtgtgt
     2041 cacttcaacg caatttgata ataatcataa tactatatgt gattatgatc cctatccaaa
     2101 gttgcggttc tataaaaata ccattcaaga gcgtatacat ctttatccat cagtgtctta
     2161 attttgtcta tagctaaaaa aaggttcaat tgtaaaagga atatggagtc ttaccaaaac
     2221 cagtccggag cgcagcagac tcaccaatag cttgaccaat ttggaaatcc atttccagcc
     2281 accaccggag cctatggaac tgccggtgga gctccagccg tggctgaagg tggtggttta
     2341 agtggcatgc ttcaccgttc tgggagtagc tctagctcta gctcggtatg cttctgtttt
     2401 acgcaattgc ttttgcaata tttagtgtac tctttctata tcaccaaata caagaagttt
     2461 catttaattt gacttcacct aactagaaaa aatcaggaaa ccatatttta gatttaactc
     2521 tacatatttt ttataaacca ttttttgtgt tcaaatgata agtttttgtt tgtaatttga
     2581 attatattca aggtatacca tatatttaaa attactaaat aaaatatgga aagtgttgca
     2641 aatacatata gctttttaat ttttctgacg attttcatgc atgaaggagg atgatggact
     2701 aggtggaagg aggaggaaga agaagggaat aacggagaag attaaagaga agctgccggg
     2761 tcatcacgac tccaacaaga cttcttcttt aggttctaca acgacggcct atgataccgg
     2821 caccgttcac cacgagaaga aaggcatgat ggagaagatc aaagagaagc ttccgggtgg
     2881 tcatcattag tttcatttta attaacttgt ttggtgtggg tcgttacgct tatggtcgtt
     2941 gcatggaata aattataaac cctctctttt tcgtaacatt tatggctcag gctctattgt
     3001 tttgagtgat acaaattaag gtaatgttag cacacgggtg ggttcacgat tctaatcgac
     3061 atcttcccat ttaaaataac aaagggattt tgacgttttc tattcccaac acaaaggaag
     3121 acaaaagaag gagtgaaaat cggtaactat atatagatgt aacgaatctt atatccaaac
     3181 caacactcac aattaatgta tccccccatc caacaaatta aaagccacgt tgacgtgaca
     3241 aatcatactt ggtgacgcct tcctctataa attatccaac catgcattca tcgtcattcc
     3301 cacaataatc gctgagagtc caacaagttt gtacaaaaaa gttgaacgag aaacgtaaaa
     3361 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
     3421 taaaacacaa catatgcagt cactatgaac caactactta gatggtatta gtgacctgta
     3481 gaattcgagc tctagagctg cagggcggcc gcgatatccc ctatagtgag tcgtattaca
     3541 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg
     3601 cacaagataa aaatatatca tcatgaacaa taaaactgtc tgcttacata aacagtaata
     3661 caaggggtgt tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca
     3721 tggatgctga tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga
     3781 caatctatcg cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag
     3841 gtagcgttgc caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta
     3901 tgcctcttcc gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca
     3961 ctgcgatccc cggaaaaaca gcattccagg tattagaaga atatcctgat tcaggtgaaa
     4021 atattgttga tgcgctggca gtgttcctgc gccggttgca ttcgattcct gtttgtaatt
     4081 gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc gcaatcacga atgaataacg
     4141 gtttggttga tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct
     4201 ggaaagaaat gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt
     4261 tctcacttga taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac
     4321 gagtcggaat cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt
     4381 tttctccttc attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga
     4441 ataaattgca gtttcatttg atgctcgatg agtttttcta atcagaattg gttaattggt
     4501 tgtaacactg gcagagcatt acgctgactt gacgggacgg cgcaagctca tgaccaaaat
     4561 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4621 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4681 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4741 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     4801 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4861 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4921 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4981 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5041 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5101 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5161 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5221 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgtt
//
