LOCUS       sequence-257608-       15881 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Fluorescent reporter for subcellular localisation and expression 
            analysis of RUBY.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15881)
  AUTHORS   Sola K, Gilchrist EJ, Ropartz D, Wang L, Feussner I, Mansfield SD, 
            Ralet MC, Haughn GW
  TITLE     RUBY, a Putative Galactose Oxidase, Influences Pectin Properties and
            Promotes Cell-To-Cell Adhesion in the Seed Coat Epidermis of 
            Arabidopsis.
  JOURNAL   Plant Cell. 2019 Apr;31(4):809-831. doi: 10.1105/tpc.18.00954. Epub 
            2019 Mar 8.
  PUBMED    30852555
REFERENCE   2  (bases 1 to 15881)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.18.00954"; journalName: "Plant Cell"; date: "2019-04"; 
            volume: "31"; issue: "4"; pages: "809-831"
COMMENT     Sequence Label: pCambia2300 - ProRUBY:RUBY-Citrine
FEATURES             Location/Qualifiers
     source          1..15881
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..17
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1..17
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             join(6192..6194,6195..6905)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6234..6255)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6495..6514)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6842..6863
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      6955..7207
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(7225..7242)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7225..7241)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7234..7256)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    7444..7468
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             8769..9398
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             9827..10900
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      10966..11160
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(11396..11418)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    11504..11644
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     11559..11578
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(11659..11676)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11830..12418)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11910..11929)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(12505..13299)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    13724..13748
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    13826..14000
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(14057..14854)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(14168..14187)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     14778..14797
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(14917..15593)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(14960..14980)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    15784..15805
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(15820..15825,15826..15843,15844..15850)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15858..15874
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     join(15863..15881,1..4)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
ORIGIN
        1 caggaaacag ctatgacatg attacgaatt cgctaaccca atctcaatcg aaccggacca
       61 aattagaatg aaatggtttt agtgagttac atggttctta atttgactgg atgaaattaa
      121 ttaaaggcac tatatagatg aaactaaaac tacaagaaca ttgctctaac ttttacacaa
      181 ctttaaaaaa aaaaaaaaaa aaatcgtgga atgttgattc atttgacctt tattctggat
      241 tgaaagtgat gcaaaaatga ctttatctaa taaataaaaa aaagaagact ttatctgatg
      301 tagtgaaatt cttgtatttg ttgatgcaat gatgctatgg attgttttca aatgtttcaa
      361 ttacgtatca aattatttta tttgtaccaa cacatgcaag tatgcaacca tggcctcgtc
      421 gtaaagaact ttgggcaaca tacaaatttg ggcaaacatc tataacacat tggatttttt
      481 tgatcgattt aatttgattc ttggaattac tattaccttc ttcttttaaa aattcagtaa
      541 gatatgtgtt accgtgtgaa agtctatatg tgtgggaacg aaaggtttga agacgagtaa
      601 gaaagtagaa gagcacaatg cgaaacttta ttggtatacg taactgttta ttgacgacat
      661 ttgtaggaac aaaaattgca agataacaca ttttttagtg tttgctttgc ttgtttatag
      721 taaccggtag gcagtggcgg aggcagaata attagataca ggggtcactc tttttgtaag
      781 ccaaatacca aaaaaaaaaa attaagaaaa atgataatat cctcataaat gataatttca
      841 aaaaaaaata tatatatgag aaagaaaaaa actgttctag taaagtaaac attaaatcta
      901 aagtaattta tactgtgtta aacaataata atgagattaa cttagtttaa tagacttatt
      961 tttttattta taaaattaat tgggttaatt tgttgtaggt ttaatatttt ttgttagact
     1021 atatttgaat atatattgat gggggtcaat aatattttgg gaaagggtca atttataaaa
     1081 attaacaaaa cctaaagtaa ttttctaaaa tccatagggg tcaattgacc cccatgatta
     1141 tacactccct ccgccactgc cggtagggtt tgatgtcttg gattgttaag ttttaaagtt
     1201 ttgtctcatg atctatgtaa gtgcttaagc cggcgctagc ataaattgaa cctttaatca
     1261 gaatgtagta gttgacctgg atggatgaca cttttatgcg agatgggtcg agcgacgcta
     1321 accattaggc cctcgatcgg cagtcggcag atataactta gatacagtgg atagtgacat
     1381 atcctcttaa taaatatgtt ttaaatgtta gattaactta tgacctaaag aaaagaagtt
     1441 agattaacat tttttagaaa ttattatttc ggataggttt aataagcata agtcagcgaa
     1501 acttataagg tatttattta caattataaa atcaacagct tccaaacaaa atcttttttc
     1561 tttttgactt cttaaaacaa aatctcatac taaccgattt aacattatgt ttgtgaataa
     1621 tatggtaacg cgaatgatta gcaacataaa aaacaaagag tcaactaacc gcattcatga
     1681 atggaatgga atgtcacggt caatcattag atttggtgtt acggaataca tatttattaa
     1741 atgtatatga agaaaaaagc atggccaata ataatgtaaa taaattggct caacatgcga
     1801 tgtatgtaca taatttagag tggaaaatga aaagatatat acaatgtgta ttaatataga
     1861 tgtacgcagt tgtataaaat tgatggacat gatttaggtt tttttttaaa aaattgatgg
     1921 acatttttca aatatcaaaa tgtttaattt atattttcta catataattg aaattaaaca
     1981 aaggagatat aagttctaca taattttact ttgtatcgtt ggaacaaaca cgtacagtag
     2041 tattatttta tatatgtgca tgtatgtttt gcgttccttt aaaaatcaaa tacatgggaa
     2101 gataacatgc aacgaaccag atgatacagc gcctgatgag aggtcaaaaa cgtaacgagt
     2161 ttgacttgta taatattaat atgtagttgc atgtttgatg ttgacacagc atcgccatca
     2221 ttaattcgtt ctctgcagtc taccgaacta attaatctca aataatcgat gagtacatat
     2281 atgttaagtc cgtcgaggct aatgtataat tcaataatat aaatatgttc attcgaggat
     2341 atagttcata ttattatata tctgtttaaa attattgact ccaatcatcc tataaactaa
     2401 gagggttgac accgcagcaa agaaaaaaat aaaatatacc ataattcata tatttgcaca
     2461 atctgaatta taatggaaat taagtttgct caaacaaaaa aaaagggttg gctccatata
     2521 cgaaattgtt aatttgtaat tttgtaggac ttctaattaa aaaaatatat atcaataaat
     2581 gattatttta tatatgtcaa ttatatctga tcattaatta attaattgtt tcgaaaataa
     2641 ttaattaatt ttgaaattta aattaaagca atattaaatg taaaaatttt aaattatatt
     2701 aaactagaaa tttattgtga gcatttttgt gtatagatca agatatgatg aatcttgtgg
     2761 gatttttttt attctataac aggtgttgtt tcatatgcta cgcattttta tatatttaac
     2821 caaacataat gacaagttga caacgactag tatttcttcc catttagatg ataagattag
     2881 tccaaatact ccaaaccgtc tgttggatta agaatgaccg gaatacgatt actccaaaac
     2941 gcatatgggt ttactggtcg agaatatacc tttggattaa gatcatgtag agttatttca
     3001 ttacgatttt gatttctttt ggttattgtg aatatgtttt ttactttctt ttttctgtta
     3061 gagatcattt gtgaatatgt taagtggtaa gtgtacaatt tttcatattt tgaatcgagt
     3121 atcataaata tcattgtaca cataccagtt tatttatttc gtcctgatat taacaagtaa
     3181 caacgtcaat tttgagatca attatttgaa aatatagaaa actgaaagta ttaaccaatt
     3241 tagtttgatt ctaattagag tcatcgtatt gtgctagtaa tcggtgacgg gaaccacata
     3301 aaacgacagt ttatcaacat aaaaaaaaat cacaaacaca tagaagactc tagttttgtt
     3361 ataaacttgt gctaaataat taacacgata cataatactc ttcattatta tatatacttt
     3421 tcatgtataa taatgcatgg acattacaca aatggaatga atagtacaat tgtatgtgca
     3481 tgaatgtttt tctaaaaact gacatccgtt tgtatgctat tttatccttg tttgttattt
     3541 aatagtagaa aacttgcgag gaccaaaata attagcatgc aaagaaccag aagaaatcgt
     3601 gagaactaaa acgtaaagaa ctcttgtgac ttgtataata tatagtttgt atgcatgttt
     3661 gatgttgaca caacatatct ccatcattaa ttcgtccctt gcagtctctc aaacttatta
     3721 atctcaaata atcgatgttt atagattaag tccctcgaga ctaataatgt aaatccaatg
     3781 atacaatgtg gttccttcga cgacgaatca tattctgatt tctgtatatc tgtaatataa
     3841 agtacgatta ttattatatt tgaatacaat catacttaat aaaatattgt gtcatatata
     3901 tttatctcca taaatgtttc tcttgactga aatgtgaata cccaaacata aaaacatata
     3961 cagtaaatac tttgttcaat tcccattaaa tttctctttg aatctaaaat cagacataga
     4021 aatactgttg tttagagaaa gattttgaat atatagaaca ttaaatcgcc gcagaacttt
     4081 gttgtttaga gaaagatttt aaatatgata agaactcaac taaagaaaaa gaaaaaaaat
     4141 tatgacgtct caatttagag ttttattggt tcttttgtac agttctgtaa tgttaggaat
     4201 cgttttatac acaaaggtaa cttaaatctt tcaaacgtct acggtagtgg acggtcatta
     4261 aaatcagagt taagaaaacg aacaaaccaa atttaatcat atcttataca ctcgagacct
     4321 tgattatata ttactctttt atatattaac agaactagaa ataaaaacta tacattatta
     4381 gaatatcgta acgtatacta atatcccatc gaccagtata taaacgacac aagtcacagt
     4441 acattctcat cacataccac aaaatatctc ataaagatca aataaacact aaacctgaaa
     4501 gcaaaaaaat ggcagcacga gcaacattca tattctatgc tctcttccta accacactcc
     4561 aattccttct aacacaccat gtgtcatccg ccgcccgagg attatggaaa tacatcgccc
     4621 ccaacgtcgg aatctccgcg atgcatatgc aacttctcca taacgaccgt gtcgtaatgt
     4681 acgaccgaac caacttcgga ccatcaaaca tctctctacc taacggtaac tgccgtgaca
     4741 acccccaaga cgccgtctcc aaaatcgact gcacagctca ctcgatcgaa tacgacgtcg
     4801 caacgaacac catccgacct ttaacggtac aatccaacac atggtgctca tccggttcgg
     4861 ttcgaccgga cggtgttctt gtccaaaccg gtggagaccg agacggagaa cttaagacaa
     4921 gaaccttttc tccttgtaat aacaatcaat gtgattgggt cgaaatgaac aacggtttaa
     4981 aaaagagaag atggtacgct tccaaccata ttcttcccga cggtaaacaa atcgtcatgg
     5041 gaggtcaagg acaattcaac tacgagttct ttcccaaaac gacaaaccct aacgtagtcg
     5101 cgttaccgtt tttggccgag actcatgatc aaggacaaga gaataatctt tatccttttg
     5161 tgtttatgaa taccgatggg aatctattta tgttcgctaa taacagagcg atattactcg
     5221 actatgttaa gaacacggtg gtgaaaactt ttccggcgat tcccggtggt gatccgagaa
     5281 actatccgag cactggctca gccgtgttat taccgttaaa gaatcttgaa gcggataatg
     5341 tcgagacgga ggttctagtg tgtggaggtg caccaaaagg atcatacaac ctcgctagaa
     5401 agaaaacttt cgtgaaagcg cttgacacgt gtgcaaggat caagattaac gatgccaaac
     5461 ctgaatgggc ggttgagaag atgccacatg ctagagttat gggagatatg attcctttgc
     5521 caaacggaga tgttttgcta atcaacggtg gatcttttgg tactgctgca tgggagcttg
     5581 gtcgtacacc ggttttagca ccagacctat accatcccga gaatccggtt ggctctaggt
     5641 ttgaatcgct aaggccgact acaatcccga gaatgtatca ctccgcggct attcttctac
     5701 gtgacggtag ggttttagtc ggaggaagca atcctcatgc gttttataac tacaccggag
     5761 tgcttttccc gacggagttg agcttagaag ctttctctcc ggtttaccta caacgggagt
     5821 tttcgaatct tcgtcccaag attatatctc ctgaacctca gtcgatgatt aaatatggga
     5881 cgaatttgaa gttgaaattt tcggtcaccg gagaagttac gactccggcg aaagtgacta
     5941 tggtatttcc gacgttcacg acgcatagct tcgcgatgaa tcagagggtt ttggttttgg
     6001 acaatgttaa gtttacgagg aaaggcaaat cgccgatgta tgaggttcaa gtgaggactc
     6061 cgaggtcggc gaatattgca tggcctggtt attatatgat ttttgtggtg aatcaagaca
     6121 taccaagcga aggtgtttgg gtaaagttag aaggaggtct agagtcgacc cctggaggtg
     6181 gaggtggagc tgtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
     6241 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg
     6301 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
     6361 ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc taccccgacc
     6421 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
     6481 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg
     6541 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
     6601 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc
     6661 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc
     6721 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
     6781 acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
     6841 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt
     6901 acaaggatcc tgctggtgct gctgcgcctc tagcataatc tagagaattt ccccgatcgt
     6961 tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct tgcgatgatt
     7021 atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta atgcatgacg
     7081 ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta atacgcgata
     7141 gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc atctatgtta
     7201 ctcctgcagg catgcaagct tggcactggc cgtcgtttta caacgtcgtg actgggaaaa
     7261 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     7321 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     7381 ctagagcagc ttgagcttgg atcagattgt cgtttcccgc cttcagttta aactatcagt
     7441 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     7501 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     7561 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
     7621 cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt
     7681 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     7741 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     7801 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
     7861 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
     7921 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
     7981 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
     8041 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
     8101 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
     8161 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
     8221 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
     8281 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
     8341 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
     8401 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
     8461 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
     8521 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg
     8581 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
     8641 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     8701 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
     8761 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     8821 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     8881 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     8941 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     9001 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
     9061 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
     9121 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
     9181 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
     9241 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
     9301 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
     9361 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     9421 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     9481 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     9541 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     9601 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     9661 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     9721 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct
     9781 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
     9841 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     9901 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     9961 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
    10021 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
    10081 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
    10141 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
    10201 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
    10261 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
    10321 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
    10381 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
    10441 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
    10501 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
    10561 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
    10621 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
    10681 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
    10741 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
    10801 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
    10861 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
    10921 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta
    10981 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
    11041 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
    11101 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
    11161 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
    11221 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
    11281 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
    11341 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
    11401 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
    11461 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
    11521 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
    11581 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
    11641 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
    11701 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
    11761 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
    11821 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
    11881 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
    11941 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
    12001 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
    12061 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
    12121 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
    12181 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
    12241 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
    12301 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
    12361 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
    12421 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
    12481 gattttggtc atgcattcta ggtactaaaa caattcatcc agtaaaatat aatattttat
    12541 tttctcccaa tcaggcttga tccccagtaa gtcaaaaaat agctcgacat actgttcttc
    12601 cccgatatcc tccctgatcg accggacgca gaaggcaatg tcataccact tgtccgccct
    12661 gccgcttctc ccaagatcaa taaagccact tactttgcca tctttcacaa agatgttgct
    12721 gtctcccagg tcgccgtggg aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa
    12781 atcatacagc tcgcgcggat ctttaaatgg agtgtcttct tcccagtttt cgcaatccac
    12841 atcggccaga tcgttattca gtaagtaatc caattcggct aagcggctgt ctaagctatt
    12901 cgtataggga caatccgata tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag
    12961 ctcgataatc ttttcagggc tttgttcatc ttcatactct tccgagcaaa ggacgccatc
    13021 ggcctcactc atgagcagat tgctccagcc atcatgccgt tcaaagtgca ggacctttgg
    13081 aacaggcagc tttccttcca gccatagcat catgtccttt tcccgttcca catcataggt
    13141 ggtcccttta taccggctgt ccgtcatttt taaatatagg ttttcatttt ctcccaccag
    13201 cttatatacc ttagcaggag acattccttc cgtatctttt acgcagcggt atttttcgat
    13261 cagttttttc aattccggtg atattctcat tttagccatt tattatttcc ttcctctttt
    13321 ctacagtatt taaagatacc ccaagaagct aattataaca agacgaactc caattcactg
    13381 ttccttgcat tctaaaacct taaataccag aaaacagctt tttcaaagtt gttttcaaag
    13441 ttggcgtata acatagtatc gacggagccg attttgaaac cgcggtgatc acaggcagca
    13501 acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg tgtttcaaac
    13561 ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa gtctgccgcc
    13621 ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta tcgagtggtg
    13681 attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg atatattgtg
    13741 gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt ttaatgtact
    13801 gaattaacgc cgaattaatt cgggggatct ggattttagt actggatttt ggttttagga
    13861 attagaaatt ttattgatag aagtatttta caaatacaaa tacatactaa gggtttctta
    13921 tatgctcaac acatgagcga aaccctatag gaaccctaat tcccttatct gggaactact
    13981 cacacattat tatggagaaa ctcgagcttg tcgatcgact ctagctagag gatcgatccg
    14041 aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
    14101 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
    14161 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
    14221 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
    14281 catcgccatg tgtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga
    14341 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
    14401 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
    14461 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
    14521 cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc aatagcagcc
    14581 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
    14641 ccagccacga tagccgcgct gcctcgtcct ggagttcatt cagggcaccg gacaggtcgg
    14701 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg aaacacggcg gcatcagagc
    14761 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
    14821 aacctgcgtg caatccatct tgttcaatcc ccatggtcga tcgacagatc tgcgaaagct
    14881 cgagagagat agatttgtag agagagactg gtgatttcag cgtgtcctct ccaaatgaaa
    14941 tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct
    15001 tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
    15061 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
    15121 atcttgaacg atagcctttc ctttatcgca atgatggcat ttgtaggtgc caccttcctt
    15181 ttctactgtc cttttgatga agtgacagat agctgggcaa tggaatccga ggaggtttcc
    15241 cgatattacc ctttgttgaa aagtctcaat agccctttgg tcttctgaga ctgtatcttt
    15301 gatattcttg gagtagacga gagtgtcgtg ctccaccatg ttcacatcaa tccacttgct
    15361 ttgaagacgt ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat
    15421 ctttgggacc actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat
    15481 ggcatttgta ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg
    15541 ggcaatggaa tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc
    15601 tttggtcttc tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca
    15661 ccatgttggc aagctgctct agccaatacg caaaccgcct ctccccgcgc gttggccgat
    15721 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
    15781 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
    15841 tcgtatgttg tgtggaattg tgagcggata acaatttcac a
//
