LOCUS       sequence-257611-       10979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conversion of C to T monocotyledons protoplasts .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10979)
  AUTHORS   Zong Y, Song Q, Li C, Jin S, Zhang D, Wang Y, Qiu JL, Gao C
  TITLE     Efficient C-to-T base editing in plants using a fusion of nCas9 and 
            human APOBEC3A.
  JOURNAL   Nat Biotechnol. 2018 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261.
  PUBMED    30272679
REFERENCE   2  (bases 1 to 10979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261."
COMMENT     Sequence Label: A3A-Gam
FEATURES             Location/Qualifiers
     source          1..10979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(3290..3313,3314..3316,3317..7390)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7391..7438
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             7466..7714
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             7742..7990
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             8003..8023
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8097..8273
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     complement(8827..8843)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8827..8843)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8840..8862)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8851..8867
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8875..8881,8882..8899,8900..8905))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8920..8941
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9058..9075)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9229..9817)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9309..9328)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9988..10779,10780..10848))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10611..10630
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10849..10953)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gagctcggta cctgacccgg tcgtgcccct ctctagagat aatgagcatt gcatgtctaa
       61 gttataaaaa attaccacat attttttttg tcacacttgt ttgaagtgca gtttatctat
      121 ctttatacat atatttaaac tttactctac gaataatata atctatagta ctacaataat
      181 atcagtgttt tagagaatca tataaatgaa cagttagaca tggtctaaag gacaattgag
      241 tattttgaca acaggactct acagttttat ctttttagtg tgcatgtgtt ctcctttttt
      301 tttgcaaata gcttcaccta tataatactt catccatttt attagtacat ccatttaggg
      361 tttagggtta atggttttta tagactaatt tttttagtac atctatttta ttctatttta
      421 gcctctaaat taagaaaact aaaactctat tttagttttt ttatttaata atttagatat
      481 aaaatagaat aaaataaagt gactaaaaat taaacaaata ccctttaaga aattaaaaaa
      541 actaaggaaa catttttctt gtttcgagta gataatgcca gcctgttaaa cgccgtcgac
      601 gagtctaacg gacaccaacc agcgaaccag cagcgtcgcg tcgggccaag cgaagcagac
      661 ggcacggcat ctctgtcgct gcctctggac ccctctcaat cgagagttcc gctccaccgt
      721 tggacttgct ccgctgtcgg catccagaaa ttgcgtggcg gagcggcaga cgtgagccgg
      781 cacggcaggc ggcctcctcc tcctctcacg gcaccggcag ctacggggga ttcctttccc
      841 accgctcctt cgctttccct tcctcgcccg ccgtaataaa tagacacccc ctccacaccc
      901 tctttcccca acctcgtgtt gttcggagcg cacacacaca caaccagatc tcccccaaat
      961 ccacccgtcg gcacctccgc ttcaaggtac gccgctcgtc ctcccccccc ccccctctct
     1021 accttctcta gatcggcgtt ccggtccatg gttagggccc ggtagttcta cttctgttca
     1081 tgtttgtgtt agatccgtgt ttgtgttaga tccgtgctgc tagcgttcgt acacggatgc
     1141 gacctgtacg tcagacacgt tctgattgct aacttgccag tgtttctctt tggggaatcc
     1201 tgggatggct ctagccgttc cgcagacggg atcgatttca tgattttttt tgtttcgttg
     1261 catagggttt ggtttgccct tttcctttat ttcaatatat gccgtgcact tgtttgtcgg
     1321 gtcatctttt catgcttttt tttgtcttgg ttgtgatgat gtggtctggt tgggcggtcg
     1381 ttctagatcg gagtagaatt aattctgttt caaactacct ggtggattta ttaattttgg
     1441 atctgtatgt gtgtgccata catattcata gttacgaatt gaagatgatg gatggaaata
     1501 tcgatctagg ataggtatac atgttgatgc gggttttact gatgcatata cagagatgct
     1561 ttttgttcgc ttggttgtga tgatgtggtg tggttgggcg gtcgttcatt cgttctagat
     1621 cggagtagaa tactgtttca aactacctgg tgtatttatt aattttggaa ctgtatgtgt
     1681 gtgtcataca tcttcatagt tacgagttta agatggatgg aaatatcgat ctaggatagg
     1741 tatacatgtt gatgtgggtt ttactgatgc atatacatga tggcatatgc agcatctatt
     1801 catatgctct aaccttgagt acctatctat tataataaac aagtatgttt tataattatt
     1861 ttgatcttga tatacttgga tgatggcata tgcagcagct atatgtggat ttttttagcc
     1921 ctgccttcat acgctattta tttgcttggt actgtttctt ttgtcgatgc tcaccctgtt
     1981 gtttggtgtt acttctgcaa agcttgtcga cggatccatg gcgaagccgg ccaagaggat
     2041 caaatccgct gctgctgcct acgtgccgca aaatagggat gccgtgatca ccgacatcaa
     2101 gaggatcggc gatctgcaga gggaggcgtc tcgtctcgaa actgagatga acgacgcgat
     2161 cgcggagatc accgagaagt tcgccgctcg tatcgccccg atcaagaccg acatcgaaac
     2221 tctctccaag ggcgtgcaag gttggtgcga ggccaatagg gacgagctca ccaatggcgg
     2281 caaggtgaag accgccaacc tcgtgaccgg cgatgtgtct tggagggtga ggccaccatc
     2341 cgtgagcatt cgtggtatgg acgccgtgat ggaaactctc gagcgcctcg gcctccaaag
     2401 gttcatccgc accaagcaag aaatcaacaa ggaggcgatc ctcctcgagc caaaagccgt
     2461 ggccggcgtg gccggcatca cagtcaagtc cggcatcgag gacttctcca tcatcccgtt
     2521 cgagcaagaa gccggcatct ccggcagcga gacgccaggc acctccgaga gcgctacgcc
     2581 tgaatccagg cctgaggcca gcccggctag cggcccaagg catctcatgg acccgcacat
     2641 cttcaccagc aacttcaaca acggcatcgg caggcacaag acctacttgt gctacgaggt
     2701 ggagaggctc gacaacggaa cctccgtgaa gatggaccaa cacagggggt tcctccacaa
     2761 ccaagccaag aacctcctct gcggcttcta cggcaggcac gccgagttga ggttcctcga
     2821 cttggtgcca tccctccaac tcgatccagc ccaaatctac cgcgtgacct ggttcatctc
     2881 ctggtcccca tgcttctcct ggggttgcgc cggcgaggtt cgggctttcc tccaagaaaa
     2941 cacccacgtc cgcctccgca ttttcgccgc caggatctat gattacgacc ctctctacaa
     3001 ggaggccctc cagatgctgc gggacgccgg tgctcaggtg agtatcatga cctacgacga
     3061 gttcaagcac tgctgggaca ccttcgttga ccaccagggc tgcccattcc aaccatggga
     3121 cggtctggat gaacacagcc aagccttgtc cggcaggctc cgggccatcc tccaaaacca
     3181 ggggaacagc ggaggatctt ccggaggatc tagcggctcc gagacaccag gaacatccga
     3241 aagcgctaca ccagaatcta gcggaggctc ttccggagga tctcttaagg acaagaagta
     3301 ctcgatcggc ctcgccatcg ggacgaactc agttggctgg gccgtgatca ccgacgagta
     3361 caaggtgccc tctaagaagt tcaaggtcct ggggaacacc gaccgccatt ccatcaagaa
     3421 gaacctcatc ggcgctctcc tgttcgacag cggggagacc gctgaggcta cgaggctcaa
     3481 gagaaccgct aggcgccggt acacgagaag gaagaacagg atctgctacc tccaagagat
     3541 tttctccaac gagatggcca aggttgacga ttcattcttc caccgcctgg aggagtcttt
     3601 cctcgtggag gaggataaga agcacgagcg gcatcccatc ttcggcaaca tcgtggacga
     3661 ggttgcctac cacgagaagt accctacgat ctaccatctg cggaagaagc tcgtggactc
     3721 caccgataag gcggacctca gactgatcta cctcgctctg gcccacatga tcaagttccg
     3781 cggccatttc ctgatcgagg gggatctcaa cccagacaac agcgatgttg acaagctgtt
     3841 catccaactc gtgcagacct acaaccaact cttcgaggag aacccgatca acgcctctgg
     3901 cgtggacgcg aaggctatcc tgtccgcgag gctctcgaag tccaggaggc tggagaacct
     3961 gatcgctcag ctcccaggcg agaagaagaa cggcctgttc gggaacctca tcgctctcag
     4021 cctggggctc accccgaact tcaagtcgaa cttcgatctc gctgaggacg ccaagctgca
     4081 actctccaag gacacctacg acgatgacct cgataacctc ctggcccaga tcggcgatca
     4141 atacgcggac ctgttcctcg ctgccaagaa cctgtcggac gccatcctcc tgtcagatat
     4201 cctccgcgtg aacaccgaga tcacgaaggc tccactctct gcctccatga tcaagcgcta
     4261 cgacgagcac catcaggatc tgaccctcct gaaggcgctg gtccgccaac agctcccgga
     4321 gaagtacaag gagattttct tcgatcagtc gaagaacggc tacgctgggt acatcgacgg
     4381 cggggcctca caagaggagt tctacaagtt catcaagcca atcctggaga agatggacgg
     4441 cacggaggag ctcctggtga agctcaacag ggaggacctc ctgcggaagc agagaacctt
     4501 cgataacggc agcatccccc accaaatcca tctcggggag ctgcacgcca tcctgagaag
     4561 gcaagaggac ttctaccctt tcctcaagga taaccgggag aagatcgaga agatcctgac
     4621 cttcagaatc ccatactacg tcggccctct cgcgcggggg aactcaagat tcgcttggat
     4681 gacccgcaag tctgaggaga ccatcacgcc gtggaacttc gaggaggtgg tggacaaggg
     4741 cgctagcgct cagtcgttca tcgagaggat gaccaacttc gacaagaacc tgcccaacga
     4801 gaaggtgctc cctaagcact cgctcctgta cgagtacttc accgtctaca acgagctcac
     4861 gaaggtgaag tacgtcaccg agggcatgcg caagccagcg ttcctgtccg gggagcagaa
     4921 gaaggctatc gtggacctcc tgttcaagac caaccggaag gtcacggtta agcaactcaa
     4981 ggaggactac ttcaagaaga tcgagtgctt cgattcggtc gagatcagcg gcgttgagga
     5041 ccgcttcaac gccagcctcg ggacctacca cgatctcctg aagatcatca aggataagga
     5101 cttcctggac aacgaggaga acgaggatat cctggaggac atcgtgctga ccctcacgct
     5161 gttcgaggac agggagatga tcgaggagcg cctgaagacg tacgcccatc tcttcgatga
     5221 caaggtcatg aagcaactca agcgccggag atacaccggc tgggggaggc tgtcccgcaa
     5281 gctcatcaac ggcatccggg acaagcagtc cgggaagacc atcctcgact tcctcaagag
     5341 cgatggcttc gccaacagga acttcatgca actgatccac gatgacagcc tcaccttcaa
     5401 ggaggatatc caaaaggctc aagtgagcgg ccagggggac tcgctgcacg agcatatcgc
     5461 gaacctcgct ggctcccccg cgatcaagaa gggcatcctc cagaccgtga aggttgtgga
     5521 cgagctcgtg aaggtcatgg gccggcacaa gcctgagaac atcgtcatcg agatggccag
     5581 agagaaccaa accacgcaga aggggcaaaa gaactctagg gagcgcatga agcgcatcga
     5641 ggagggcatc aaggagctgg ggtcccaaat cctcaaggag cacccagtgg agaacaccca
     5701 actgcagaac gagaagctct acctgtacta cctccagaac ggcagggata tgtacgtgga
     5761 ccaagagctg gatatcaacc gcctcagcga ttacgacgtc gatcatatcg ttccccagtc
     5821 tttcctgaag gatgactcca tcgacaacaa ggtcctcacc aggtcggaca agaaccgcgg
     5881 caagtcagat aacgttccat ctgaggaggt cgttaagaag atgaagaact actggaggca
     5941 gctcctgaac gccaagctga tcacgcaaag gaagttcgac aacctcacca aggctgagag
     6001 aggcgggctc tcagagctgg acaaggccgg cttcatcaag cggcagctgg tcgagaccag
     6061 acaaatcacg aagcacgttg cgcaaatcct cgactctcgg atgaacacga agtacgatga
     6121 gaacgacaag ctgatcaggg aggttaaggt gatcaccctg aagtctaagc tcgtctccga
     6181 cttcaggaag gatttccagt tctacaaggt tcgcgagatc aacaactacc accatgccca
     6241 tgacgcttac ctcaacgctg tggtcggcac cgctctgatc aagaagtacc caaagctgga
     6301 gtccgagttc gtgtacgggg actacaaggt ttacgatgtg cgcaagatga tcgccaagtc
     6361 ggagcaagag atcggcaagg ctaccgccaa gtacttcttc tactcaaaca tcatgaactt
     6421 cttcaagacc gagatcacgc tggccaacgg cgagatccgg aagagaccgc tcatcgagac
     6481 caacggcgag acgggggaga tcgtgtggga caagggcagg gatttcgcga ccgtccgcaa
     6541 ggttctctcc atgccccagg tgaacatcgt caagaagacc gaggtccaaa cgggcgggtt
     6601 ctcaaaggag tctatcctgc ctaagcggaa cagcgacaag ctcatcgcca gaaagaagga
     6661 ctgggaccca aagaagtacg gcgggttcga cagccctacc gtggcctact cggtcctggt
     6721 tgtggcgaag gttgagaagg gcaagtccaa gaagctcaag agcgtgaagg agctcctggg
     6781 gatcaccatc atggagaggt ccagcttcga gaagaaccca atcgacttcc tggaggccaa
     6841 gggctacaag gaggtgaaga aggacctgat catcaagctc ccgaagtact ctctcttcga
     6901 gctggagaac ggcaggaaga gaatgctggc ttccgctggc gagctccaga aggggaacga
     6961 gctcgcgctg ccaagcaagt acgtgaactt cctctacctg gcttcccact acgagaagct
     7021 caagggcagc ccggaggaca acgagcaaaa gcagctgttc gtcgagcagc acaagcatta
     7081 cctcgacgag atcatcgagc aaatctccga gttcagcaag cgcgtgatcc tcgccgacgc
     7141 gaacctggat aaggtcctct ccgcctacaa caagcaccgg gacaagccca tcagagagca
     7201 agcggagaac atcatccatc tcttcaccct gacgaacctc ggcgctcctg ctgctttcaa
     7261 gtacttcgac accacgatcg atcggaagag atacacctcc acgaaggagg tcctggacgc
     7321 gaccctcatc caccagtcga tcaccggcct gtacgagacg aggatcgacc tctcacaact
     7381 cggcggggat aagagacccg cagcaaccaa gaaggcaggg caagcaaaga agaagaagac
     7441 gcgttcaggc ggctccggcg gctccaccaa cctgtccgac atcatcgaga aggagacggg
     7501 caagcaactc gtgatccagg agagcatcct catgctgcca gaggaggtgg aggaggtcat
     7561 cggcaacaag ccagagtccg acatcctggt gcacaccgcc tacgacgagt ccaccgacga
     7621 gaacgtcatg ctcctgacca gcgacgcccc agagtacaag ccatgggccc tcgtcatcca
     7681 ggacagcaac ggggagaaca agatcaagat gctgtcgggg acgcgtgact ccggcggcag
     7741 caccaacctg tccgacatca tcgagaagga gacgggcaag caactcgtga tccaggagag
     7801 catcctcatg ctgccagagg aggtggagga ggtcatcggc aacaagccag agtccgacat
     7861 cctggtgcac accgcctacg acgagtccac cgacgagaac gtcatgctcc tgaccagcga
     7921 cgccccagag tacaagccat gggccctcgt catccaggac agcaacgggg agaacaagat
     7981 caagatgctg tcggggggga gcccaaagaa gaagcggaag gtgtagtgta caagtaaccc
     8041 gggaattcgg tacgctgaaa tcaccagtct ctctctacaa atctatctct ctctattttc
     8101 tccataaata atgtgtgagt agtttcccga taagggaaat tagggttctt atagggtttc
     8161 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct
     8221 atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag atctcctaaa
     8281 gtccctatag atctttgtcg tgaatataaa ccagacacga gacgactaaa cctggagccc
     8341 agacgccgtt cgaagctaga agtaccgctt aggcaggagg ccgttaggga aaagatgcta
     8401 aggcagggtt ggttacgttg actcccccgt aggtttggtt taaatatgat gaagtggacg
     8461 gaaggaagga ggaagacaag gaaggataag gttgcaggcc ctgtgcaagg taagaagatg
     8521 gaaatttgat agaggtacgc tactatactt atactatacg ctaagggaat gcttgtattt
     8581 ataccctata ccccctaata accccttatc aatttaagaa ataatccgca taagcccccg
     8641 cttaaaaatt ggtatcagag ccatgaatag gtctatgacc aaaactcaag aggataaaac
     8701 ctcaccaaaa tacgaaagag ttcttaactc taaagataaa agatctttca agatcaaaac
     8761 tagttccctc acaccggtga cggggatcgc atgcgatatc tcgagatcta gcttggcgta
     8821 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8881 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     8941 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     9001 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     9061 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     9121 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     9181 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     9241 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     9301 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     9361 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     9421 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9481 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9541 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9601 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9661 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9721 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9781 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9841 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9901 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9961 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    10021 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    10081 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    10141 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    10201 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    10261 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    10321 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    10381 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    10441 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10501 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10561 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    10621 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10681 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10741 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10801 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10861 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10921 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctg
//
