LOCUS       sequence-257615-        5104 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing the 
            TBA2 (At1g62060) promoter sequence (1346 base pairs), for use in 
            Three-way Gateway cloning..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   McGee R, Dean GH, Mansfield SD, Haughn GW
  TITLE     Assessing the utility of seed coat-specific promoters to engineer 
            cell wall polysaccharide composition of mucilage.
  JOURNAL   Plant Mol Biol. 2019 Aug 17. pii: 10.1007/s11103-019-00909-8. doi: 
            10.1007/s11103-019-00909-8.
  PUBMED    31422517
REFERENCE   2  (bases 1 to 5104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol 
            Biol. 2019 Aug 17. pii: 10.1007/s11103-019-00909-8. doi: 
            10.1007/s11103-019-00909-8."
COMMENT     Sequence Label: pDONRG_P4-P1R:TBA2p
FEATURES             Location/Qualifiers
     source          1..5104
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     88..105
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(264..291)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(383..469)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     518..540
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     532..549
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     533..549
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    589..684
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    2031..2154
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2226..2245)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2227..2245)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2250..2266)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2250..2266)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2368..2387)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(2446..2465)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(3039..3572)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(3761..3766,3767..3783,3784..3789))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      4450..5038
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4939..4958
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
       61 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
      121 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
      181 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg taccgctagc
      241 caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc tgcttagttt
      301 gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt tgcttcacaa
      361 cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc gacaaacaac
      421 agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga tgcctggcag
      481 ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg ttgtaaaacg
      541 acggccagtc ttaagctcgg gcccgagtta acgctaccat ggagctccaa ataatgattt
      601 tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc ttttttataa
      661 tgccaacttt gtatagaaaa gttggttttg tgttccctac atcataccct atatatagtc
      721 acacacacct aaacacattt ttacatcatt tttaataatg tccgtgtctg tgtgtatttt
      781 cttaattgtt acagtgaaat aaataaatgt gaaagagatt tggtttaaat tagataacta
      841 ttttacttga gagtgtttaa tctgacgtac atattggtta attcagttca acgtcttata
      901 gtgacattag atattaatat tgattagata ttttcttacg taatgttctc aaatcggata
      961 aatcagtcag tcaagccatt ataataaaat attatacgaa gttataaaag aagaaaaaga
     1021 actttttact aagtttagaa tttataatat tcaattttca ctttactaac atcggtgatt
     1081 cacctaaagc attctgtttt accaagaatg tgtgtttgct ttagaattca cagtatttac
     1141 ttctcacttg atcccgtgtg tagtagacaa taaaaccatg ttgtttcgga gactcatcat
     1201 attcacaaac agattttccg atttaatagt tctttttctt cttttataat tttcatagta
     1261 ctgtatcttg ttttaatggc tttgccttga ctgactgatt tatctgattt gagaacacta
     1321 cgtaagaaaa catctaatac tattatctac tatcactata tctgtattta aataacaata
     1381 gttgtttacc aatatcaaaa ttacagaggg tgcttaactg cgtttagtat acatttttta
     1441 ttatgtaatt ttaattataa ataaaccagt ttacaatttg ggatttcttt attaggattt
     1501 cataacacac cattgttgaa aaatggagat ctaaattgac attatatatg aaagacaata
     1561 aataagatac aacgttttat ataaacaata tggattatat acaatagtta aaaaatcctt
     1621 agatgtctgt taaacacagc gttttggtga gttaactcgt atggtataag tttaaagcat
     1681 tccagtaact atattggtat caaaatctac attcatatta attcaaataa ttttttacgt
     1741 ttaccaaaat aaatccatat aaacatgcga ttgatcttgg gcaagagagg tgttaaaata
     1801 gtatcttggt atgactatta gttgaaattt gactaatgca ttcaagaatg tatatttatt
     1861 gtaagaaatg ctcacgtatc ttatttgata aagaatgctc acgtacctta ataaatacgt
     1921 gtgcatagag tttgtgtgta tatatagatg gttatgatca gggaagaaga acacaacaca
     1981 actcacctca aacagacaat ttaattccaa aagataaaaa caatcaaaag caagtttgta
     2041 caaaaaagtt gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt
     2101 gcataaaaaa cagactacat aatactgtaa aacacaacat atgcagtcac tatgaaccaa
     2161 ctacttagat ggtattagtg acctgtagaa ttcgagctct agagctgcag ggcggccgcg
     2221 atatccccta tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg
     2281 tgtctcaaaa tctctgatgt tacattgcac aagataaaaa tatatcatca tgaacaataa
     2341 aactgtctgc ttacataaac agtaatacaa ggggtgttat gagccatatt caacgggaaa
     2401 cgtcgaggcc gcgattaaat tccaacatgg atgctgattt atatgggtat aaatgggctc
     2461 gcgataatgt cgggcaatca ggtgcgacaa tctatcgctt gtatgggaag cccgatgcgc
     2521 cagagttgtt tctgaaacat ggcaaaggta gcgttgccaa tgatgttaca gatgagatgg
     2581 tcagactaaa ctggctgacg gaatttatgc ctcttccgac catcaagcat tttatccgta
     2641 ctcctgatga tgcatggtta ctcaccactg cgatccccgg aaaaacagca ttccaggtat
     2701 tagaagaata tcctgattca ggtgaaaata ttgttgatgc gctggcagtg ttcctgcgcc
     2761 ggttgcattc gattcctgtt tgtaattgtc cttttaacag cgatcgcgta tttcgtctcg
     2821 ctcaggcgca atcacgaatg aataacggtt tggttgatgc gagtgatttt gatgacgagc
     2881 gtaatggctg gcctgttgaa caagtctgga aagaaatgca taaacttttg ccattctcac
     2941 cggattcagt cgtcactcat ggtgatttct cacttgataa ccttattttt gacgagggga
     3001 aattaggccg ggaagccgat ctcggcttga acgaattgtt aggtggcggt acttgggtcg
     3061 atatcaaagt gcatcacttc ttcccgtatg cccaactttg tatagagagc cactgcggga
     3121 tcgtcaccgt aatctgcttg cacgtagatc acataagcac caagcgcgtt ggcctcatgc
     3181 ttgaggagat tgatgagcgc ggtggcaatg ccctgcctcc ggtgctcgcc ggagactgcg
     3241 agatcataga tatagatctc actacgcggc tgctcaaact tgggcagaac gtaagccgcg
     3301 agagcgccaa caaccgcttc ttggtcgaag gcagcaagcg cgatgaatgt cttactacgg
     3361 agcaagttcc cgaggtaatc ggagtccggc tgatgttggg agtaggtggc tacgtctccg
     3421 aactcacgac cgaaaagatc aagagcagcc cgcatggatt tgacttggtc agggccgagc
     3481 ctacatgtgc gaatgatgcc catacttgag ccacctaact ttgttttagg gcgactgccc
     3541 tgctgcgtaa catcgttgct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     3601 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta caaaaaaaca
     3661 gtcataacaa gccatgaaaa ccgccactgc gccgttacca ccgctgcgtt cggtcaaggt
     3721 tctggaccag ttgcgtgagc gcatacgcta cttgcattac agtttacgaa ccgaacaggc
     3781 ttatgtcaac tgggttcgtg ccttcatccg tttccacggt gtgcgtcacc cggcaacctt
     3841 gggcagcagc gaagtcgagg catttctgtc ctggctggcg aacgagcgca aggtttcggt
     3901 ctccacgcat cgtcaggcat tggcggcctt gctgttcttc tacggcaagg tgctgtgcac
     3961 ggatctgccc tggcttcagg agatcggaag acctcggccg tcgcggcgct tgccggtggt
     4021 gctgaccccg gatgaagtgg ttcgcatcct cggttttctg gaaggcgagc atcgtttgtt
     4081 cgcccaggac tctagctata gttctagtgg ttggctacta ataggttgta ttgatgttgg
     4141 acgagtcgga atcgcagacc gataccagga tcttgccatc ctatggaact gcctcggtga
     4201 gttttctcct tcattacaga aacggctttt tcaaaaatat ggtattgata atcctgatat
     4261 gaataaattg cagtttcatt tgatgctcga tgagtttttc taatcagaat tggttaattg
     4321 gttgtaacac tggcagagca ttacgctgac ttgacgggac ggcgcaagct catgaccaaa
     4381 atcccttaac gtgagttacg cgtcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     4441 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     4501 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     4561 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     4621 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     4681 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     4741 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     4801 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     4861 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     4921 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     4981 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     5041 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     5101 cttt
//
