LOCUS       sequence-257647-        4125 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing a 307
            bp MUM4 (At1g53500) promoter fragment fused to the 54 bp 35S minimal
            promoter sequence, for use in Three-way Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Dean GH, Jin Z, Shi L, Esfandiari E, McGee R, Nabata K, Lee T, Kunst
            L, Western TL, Haughn GW
  TITLE     Identification of a seed coat-specific promoter fragment from the 
            Arabidopsis MUCILAGE-MODIFIED4 gene.
  JOURNAL   Plant Mol Biol. 2017 Sep;95(1-2):33-50. doi: 
            10.1007/s11103-017-0631-7. Epub 2017 Jul 20.
  PUBMED    28730525
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol 
            Biol."; date: "2017-09"; volume: "95(1-2):33-50. doi"; pages: " 
            10.1007/s11103-017-0631-7. Epub 2017 Jul 20"
COMMENT     Sequence Label: pDONRG_P4-P1R:MUM4_0.3Pro_35S
FEATURES             Location/Qualifiers
     source          1..4125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     92..109
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(268..295)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(387..473)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     522..544
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     536..553
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    593..688
                     /gene="mutant version of attL"
                     /label=attL4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1002..1048
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
     protein_bind    1056..1179
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1251..1270)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1252..1270)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1275..1291)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1275..1291)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1393..1412)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(1471..1490)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     CDS             complement(2064..2597)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(2786..2791,2792..2808,2809..2814))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     rep_origin      3475..4063
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3964..3983
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccga gttaacgcta ccatggagct ccaaataatg
      601 attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttttt
      661 ataatgccaa ctttgtatag aaaagttgga cggtggcatt aagcatcttg cattgaatga
      721 tccgttatat ataatctcag gttttttttg ggttgaaatg atgatattaa attttaggtt
      781 gacatgtact tatctttgta atcaactaat taaatatttg aactgacatg tctacgttat
      841 atcataaata aaccaggtgt tttaattaaa taccacgatt aaccttctaa aataaggaaa
      901 atcatatttt attcgtcaat cactataatt tggaaaacga tgcaatatat ttatttcttt
      961 ctttatacac atacttaatt aattatcaaa atttctctag agcaagaccc ttcctctata
     1021 taaggaagtt catttcattt ggagaggaca cgctgcaagt ttgtacaaaa aagttgaacg
     1081 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
     1141 tacataatac tgtaaaacac aacatatgca gtcactatga accaactact tagatggtat
     1201 tagtgacctg tagaattcga gctctagagc tgcagggcgg ccgcgatatc ccctatagtg
     1261 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct
     1321 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
     1381 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg aggccgcgat
     1441 taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat aatgtcgggc
     1501 aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag ttgtttctga
     1561 aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga ctaaactggc
     1621 tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct gatgatgcat
     1681 ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa gaatatcctg
     1741 attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg cattcgattc
     1801 ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag gcgcaatcac
     1861 gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat ggctggcctg
     1921 ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat tcagtcgtca
     1981 ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta ggccgggaag
     2041 ccgatctcgg cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc
     2101 acttcttccc gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct
     2161 gcttgcacgt agatcacata agcaccaagc gcgttggcct catgcttgag gagattgatg
     2221 agcgcggtgg caatgccctg cctccggtgc tcgccggaga ctgcgagatc atagatatag
     2281 atctcactac gcggctgctc aaacttgggc agaacgtaag ccgcgagagc gccaacaacc
     2341 gcttcttggt cgaaggcagc aagcgcgatg aatgtcttac tacggagcaa gttcccgagg
     2401 taatcggagt ccggctgatg ttgggagtag gtggctacgt ctccgaactc acgaccgaaa
     2461 agatcaagag cagcccgcat ggatttgact tggtcagggc cgagcctaca tgtgcgaatg
     2521 atgcccatac ttgagccacc taactttgtt ttagggcgac tgccctgctg cgtaacatcg
     2581 ttgctgctgc gtaacatcgt tgctgctcca taacatcaaa catcgaccca cggcgtaacg
     2641 cgcttgctgc ttggatgccc gaggcataga ctgtacaaaa aaacagtcat aacaagccat
     2701 gaaaaccgcc actgcgccgt taccaccgct gcgttcggtc aaggttctgg accagttgcg
     2761 tgagcgcata cgctacttgc attacagttt acgaaccgaa caggcttatg tcaactgggt
     2821 tcgtgccttc atccgtttcc acggtgtgcg tcacccggca accttgggca gcagcgaagt
     2881 cgaggcattt ctgtcctggc tggcgaacga gcgcaaggtt tcggtctcca cgcatcgtca
     2941 ggcattggcg gccttgctgt tcttctacgg caaggtgctg tgcacggatc tgccctggct
     3001 tcaggagatc ggaagacctc ggccgtcgcg gcgcttgccg gtggtgctga ccccggatga
     3061 agtggttcgc atcctcggtt ttctggaagg cgagcatcgt ttgttcgccc aggactctag
     3121 ctatagttct agtggttggc tactaatagg ttgtattgat gttggacgag tcggaatcgc
     3181 agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt ctccttcatt
     3241 acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata aattgcagtt
     3301 tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt aacactggca
     3361 gagcattacg ctgacttgac gggacggcgc aagctcatga ccaaaatccc ttaacgtgag
     3421 ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     3481 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     3541 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     3601 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     3661 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     3721 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     3781 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     3841 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     3901 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     3961 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     4021 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     4081 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgtt
//
