LOCUS       sequence-257650-       11601 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Modified R4pGWB401 three way Gateway destination vector (Nakagawa et
            al. 2008) that enables C-terminal fusion of Citrine, a dimeric acid 
            stable yellow fluorescent protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11601)
  AUTHORS   McGee R, Dean GH, Mansfield SD, Haughn GW
  TITLE     Assessing the utility of seed coat-specific promoters to engineer 
            cell wall polysaccharide composition of mucilage.
  JOURNAL   Plant Mol Biol. 2019 Aug 17. pii: 10.1007/s11103-019-00909-8. doi: 
            10.1007/s11103-019-00909-8.
  PUBMED    31422517
REFERENCE   2  (bases 1 to 11601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol 
            Biol. 2019 Aug 17. pii: 10.1007/s11103-019-00909-8. doi: 
            10.1007/s11103-019-00909-8."
COMMENT     Sequence Label: R4pGWB401+Citrine
FEATURES             Location/Qualifiers
     source          1..11601
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    322..445
                     /gene="mutant version of attR"
                     /label=attR4
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(491..496,497..514,515..521)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             575..1234
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(641..660)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1576..1881
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1760..1779
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1922..2046)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(2081..2083,2084..2794)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(2123..2144)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2384..2403)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2731..2752
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      2844..3096
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3129..3145)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3129..3145)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3142..3164)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3153..3169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3177..3183,3184..3201,3202..3207))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3222..3243
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(3277..3529)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3919..4713)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4030..4049)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     4640..4659
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(4734..4917)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(4770..4949)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5123..5147
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5668..6459
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6705..7293
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7194..7213
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7447..7464
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7479..7619
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7545..7564)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7705..7727
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7963..8157
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8223..9296)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9725..10354)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgtgg atcccccatc acaactttgt atagaaaagt tgaacgagaa acgtaaaatg
      361 atataaatat caatatatta aattagattt tgcataaaaa acagactaca taatactgta
      421 aaacacaaca tatccagtca ctatggcggc cacatttaaa tgtcgagggg ccgcattagg
      481 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
      541 tccgtcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
      601 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
      661 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
      721 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
      781 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
      841 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
      901 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
      961 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     1021 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     1081 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     1141 tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca
     1201 acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc
     1261 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     1321 gtatacccga agtatgtcaa aaagaggtat gctatgaagc agcgtattac agtgacagtt
     1381 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     1441 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     1501 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     1561 acaggggctg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     1621 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc
     1681 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     1741 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt
     1801 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     1861 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga
     1921 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
     1981 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa
     2041 agtggttgat aacagcgctc ctggaggtgg aggtggagct gtgagcaagg gcgaggagct
     2101 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     2161 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     2221 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcggctacgg
     2281 cctgatgtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     2341 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     2401 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     2461 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     2521 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     2581 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     2641 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct
     2701 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     2761 cgggatcact ctcggcatgg acgagctgta caaggatcct gctggtgctg ctgcgcctct
     2821 agcataagag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt ttcttaagat
     2881 tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat tacgttaagc
     2941 atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt atgattagag
     3001 tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca aactaggata
     3061 aattatcgcg cgcggtgtca tctatgttac tagatcggga attggttccg gaaccaattc
     3121 gtaatcatgt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     3181 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     3241 ttaggctgaa ttaggcgcgc ctatttctga attcccgatc tagtaacata gatgacaccg
     3301 cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg tattaaatgt
     3361 ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg cattacatgt
     3421 taattattac atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg
     3481 caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatcg gggatcatcc
     3541 gggtctgtgg cgggaactcc acgaaaatat ccgaacgcag caagatatcg cggtgcatct
     3601 cggtcttgcc tgggcagtcg ccgccgacgc cgttgatgtg gacgccgggc ccgatcatat
     3661 tgtcgctcag gatcgtggcg ttgtgcttgt cggccgttgc tgtcgtaatg atatcggcac
     3721 cttcgaccgc ctgttccgca gagatcccgt gggcgaagaa ctccagcatg agatccccgc
     3781 gctggaggat catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga
     3841 aggcggcggt ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt
     3901 cgaaccccag agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg
     3961 cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag
     4021 ctcttcagca atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag
     4081 ccggccacag tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca
     4141 ggcatcgcca tgggtcacga cgagatcatc gccgtcgggc atgcgcgcct tgagcctggc
     4201 gaacagttcg gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag
     4261 accggcttcc atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg
     4321 gcaggtagcc ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt
     4381 ctcggcagga gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag
     4441 ccagtccctt cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt
     4501 ggccagccac gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc
     4561 ggtcttgaca aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga
     4621 gcagccgatt gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg
     4681 agaacctgcg tgcaatccat cttgttcaat catgcgaaac gatccagatc cggtgcagat
     4741 tatttggatt gagagtgaat atgagactct aattggatac cgaggggaat ttatggaacg
     4801 tcagtggagc atttttgaca agaaatattt gctagctgat agtgacctta ggcgactttt
     4861 gaacgcgcaa taatggtttc tgacgtatgt gcttagctca ttaaactcca gaaacccgcg
     4921 gctgagtggc tccttcaacg ttgcggttct gtcagttcca aacgtaaaac ggcttgtccc
     4981 gcgtcatcgg cgggggtcat aacgtgactc ccttaattct ccgctcatga tgaattcaga
     5041 aataaattca gcctaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat
     5101 taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac
     5161 agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc
     5221 gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc
     5281 tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt
     5341 agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc
     5401 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     5461 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     5521 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     5581 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     5641 agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga agtatcgact
     5701 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     5761 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     5821 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     5881 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     5941 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     6001 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     6061 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     6121 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     6181 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     6241 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     6301 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     6361 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     6421 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag aaattcgttc
     6481 aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat
     6541 tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa
     6601 gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg tgagttttcg
     6661 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6721 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6781 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6841 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6901 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6961 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     7021 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     7081 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     7141 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     7201 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     7261 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     7321 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     7381 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     7441 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     7501 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     7561 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     7621 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     7681 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     7741 caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc
     7801 gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca
     7861 gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta
     7921 ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg
     7981 gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct
     8041 tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg
     8101 tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga
     8161 aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta
     8221 cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc
     8281 gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc
     8341 agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag
     8401 atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag
     8461 agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc
     8521 gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac
     8581 tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg
     8641 gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg
     8701 gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc
     8761 cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc
     8821 ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg
     8881 tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct
     8941 ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg
     9001 gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa
     9061 aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc
     9121 ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt
     9181 gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg
     9241 tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg
     9301 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta
     9361 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
     9421 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
     9481 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
     9541 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
     9601 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
     9661 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
     9721 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
     9781 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
     9841 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
     9901 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
     9961 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
    10021 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
    10081 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
    10141 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
    10201 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
    10261 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
    10321 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
    10381 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
    10441 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
    10501 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
    10561 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
    10621 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
    10681 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc
    10741 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga
    10801 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg
    10861 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg
    10921 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg
    10981 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac
    11041 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt
    11101 aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg
    11161 cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc
    11221 tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc
    11281 tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg
    11341 gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca
    11401 agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac
    11461 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg
    11521 aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca
    11581 aatggacgaa cggataaacc t
//
