LOCUS       sequence-257661-       18698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Conversion of C to T in rice plants transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18698)
  AUTHORS   Zong Y, Song Q, Li C, Jin S, Zhang D, Wang Y, Qiu JL, Gao C
  TITLE     Efficient C-to-T base editing in plants using a fusion of nCas9 and 
            human APOBEC3A.
  JOURNAL   Nat Biotechnol. 2018 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261.
  PUBMED    30272679
REFERENCE   2  (bases 1 to 18698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2018 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261."
COMMENT     Sequence Label: pH-A3A-PBE
FEATURES             Location/Qualifiers
     source          1..18698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     233..255
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     247..264
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     248..264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        331..710
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     CDS             990..1781
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_RNA        1933..2008
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2312..4304
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             join(4963..4986,4987..4989,4990..9063)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             8976..8984
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             9064..9111
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9133..9381
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             9394..9414
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(10073..10089)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10073..10089)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10086..10108)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10097..10113
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10121..10127,10128..10145,10146..10151))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10166..10187
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10378..11054
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     10991..11011
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             11121..12146
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    12187..12361
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    12439..12463
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             12888..13682
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      13769..14357
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     14258..14277
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     14511..14528
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    14543..14683
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(14609..14628)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     14769..14791
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      15027..15221
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(15287..16360)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(16789..17418)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
       61 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
      121 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      181 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      241 cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc tccaagttct
      301 aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac
      361 ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag
      421 atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg
      481 ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac
      541 tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat
      601 gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta
      661 tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gtgagaccaa
      721 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
      781 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
      841 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
      901 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
      961 gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga
     1021 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     1081 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     1141 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     1201 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     1261 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     1321 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     1381 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     1441 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     1501 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     1561 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     1621 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     1681 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     1741 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt
     1801 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata
     1861 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     1921 aagccggtct cggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca
     1981 acttgaaaaa gtggcaccga gtcggtgctt ttttttttcg ttttgcattg agttttctcc
     2041 gtcgcatgtt tgcagtttta ttttccgttt tgcattgaaa tttctccgtc tcatgtttgc
     2101 agcgtgttca aaaagtacgc agctgtattt cacttattta cggcgccaca ttttcatgcc
     2161 gtttgtgcca actatcccga gctagtgaat acagcttggc ttcacacaac actggtgacc
     2221 cgctgacctg ctcgtacctc gtaccgtcgt acggcacagc atttggaatt aaagggtgtg
     2281 atcgatactg cttgctgcta agcttgcatg cctgcagtgc agcgtgaccc ggtcgtgccc
     2341 ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac atattttttt
     2401 tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa actttactct
     2461 acgaataata taatctatag tactacaata atatcagtgt tttagagaat catataaatg
     2521 aacagttaga catggtctaa aggacaattg agtattttga caacaggact ctacagtttt
     2581 atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc tatataatac
     2641 ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt tatagactaa
     2701 tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa ctaaaactct
     2761 attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa gtgactaaaa
     2821 attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc ttgtttcgag
     2881 tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc
     2941 agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg
     3001 acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt
     3061 gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc
     3121 accggcagct acgggggatt cctttcccac cgctccttcg ctttcccttc ctcgcccgcc
     3181 gtaataaata gacaccccct ccacaccctc tttccccaac ctcgtgttgt tcggagcgca
     3241 cacacacaca accagatctc ccccaaatcc acccgtcggc acctccgctt caaggtacgc
     3301 cgctcgtcct cccccccccc ccctctctac cttctctaga tcggcgttcc ggtccatggt
     3361 tagggcccgg tagttctact tctgttcatg tttgtgttag atccgtgttt gtgttagatc
     3421 cgtgctgcta gcgttcgtac acggatgcga cctgtacgtc agacacgttc tgattgctaa
     3481 cttgccagtg tttctctttg gggaatcctg ggatggctct agccgttccg cagacgggat
     3541 cgatttcatg attttttttg tttcgttgca tagggtttgg tttgcccttt tcctttattt
     3601 caatatatgc cgtgcacttg tttgtcgggt catcttttca tgcttttttt tgtcttggtt
     3661 gtgatgatgt ggtctggttg ggcggtcgtt ctagatcgga gtagaattct gtttcaaact
     3721 acctggtgga tttattaatt ttggatctgt atgtgtgtgc catacatatt catagttacg
     3781 aattgaagat gatggatgga aatatcgatc taggataggt atacatgttg atgcgggttt
     3841 tactgatgca tatacagaga tgctttttgt tcgcttggtt gtgatgatgt ggtgtggttg
     3901 ggcggtcgtt cattcgttct agatcggagt agaatactgt ttcaaactac ctggtgtatt
     3961 tattaatttt ggaactgtat gtgtgtgtca tacatcttca tagttacgag tttaagatgg
     4021 atggaaatat cgatctagga taggtataca tgttgatgtg ggttttactg atgcatatac
     4081 atgatggcat atgcagcatc tattcatatg ctctaacctt gagtacctat ctattataat
     4141 aaacaagtat gttttataat tattttgatc ttgatatact tggatgatgg catatgcagc
     4201 agctatatgt ggattttttt agccctgcct tcatacgcta tttatttgct tggtactgtt
     4261 tcttttgtcg atgctcaccc tgttgtttgg tgttacttct gcagccctag gatggaggcc
     4321 agcccggcta gcggcccaag gcatctcatg gacccgcaca tcttcaccag caacttcaac
     4381 aacggcatcg gcaggcacaa gacctacttg tgctacgagg tggagaggct cgacaacgga
     4441 acctccgtga agatggacca acacaggggg ttcctccaca accaagccaa gaacctcctc
     4501 tgcggcttct acggcaggca cgccgagttg aggttcctcg acttggtgcc atccctccaa
     4561 ctcgatccag cccaaatcta ccgcgtgacc tggttcatct cctggtcccc atgcttctcc
     4621 tggggttgcg ccggcgaggt tcgggctttc ctccaagaaa acacccacgt ccgcctccgc
     4681 attttcgccg ccaggatcta tgattacgac cctctctaca aggaggccct ccagatgctg
     4741 cgggacgccg gtgctcaggt gagtatcatg acctacgacg agttcaagca ctgctgggac
     4801 accttcgttg accaccaggg ctgcccattc caaccatggg acggtctgga tgaacacagc
     4861 caagccttgt ccggcaggct ccgggccatc ctccaaaacc aggggaactc cgggagcgag
     4921 acgccaggca cctccgagtc ggccacccca gaatctaggc ctgacaagaa gtactcgatc
     4981 ggcctcgcca ttgggactaa ctctgttggc tgggccgtga tcaccgacga gtacaaggtg
     5041 ccctcaaaga agttcaaggt cctgggcaac accgatcggc attccatcaa gaagaatctc
     5101 attggcgctc tcctgttcga cagcggcgag acggctgagg ctacgcggct caagcgcacc
     5161 gcccgcaggc ggtacacgcg caggaagaat cgcatctgct acctgcagga gattttctcc
     5221 aacgagatgg cgaaggttga cgattctttc ttccacaggc tggaggagtc attcctcgtg
     5281 gaggaggata agaagcacga gcggcatcca atcttcggca acattgtcga cgaggttgcc
     5341 taccacgaga agtaccctac gatctaccat ctgcggaaga agctcgtgga ctccacagat
     5401 aaggcggacc tccgcctgat ctacctcgct ctggcccaca tgattaagtt caggggccat
     5461 ttcctgatcg agggggatct caacccggac aatagcgatg ttgacaagct gttcatccag
     5521 ctcgtgcaga cgtacaacca gctcttcgag gagaacccca ttaatgcgtc aggcgtcgac
     5581 gcgaaggcta tcctgtccgc taggctctcg aagtctcggc gcctcgagaa cctgatcgcc
     5641 cagctgccgg gcgagaagaa gaacggcctg ttcgggaatc tcattgcgct cagcctgggg
     5701 ctcacgccca acttcaagtc gaatttcgat ctcgctgagg acgccaagct gcagctctcc
     5761 aaggacacat acgacgatga cctggataac ctcctggccc agatcggcga tcagtacgcg
     5821 gacctgttcc tcgctgccaa gaatctgtcg gacgccatcc tcctgtctga tattctcagg
     5881 gtgaacaccg agattacgaa ggctccgctc tcagcctcca tgatcaagcg ctacgacgag
     5941 caccatcagg atctgaccct cctgaaggcg ctggtcaggc agcagctccc cgagaagtac
     6001 aaggagatct tcttcgatca gtcgaagaac ggctacgctg ggtacattga cggcggggcc
     6061 tctcaggagg agttctacaa gttcatcaag ccgattctgg agaagatgga cggcacggag
     6121 gagctgctgg tgaagctcaa tcgcgaggac ctcctgagga agcagcggac attcgataac
     6181 ggcagcatcc cacaccagat tcatctcggg gagctgcacg ctatcctgag gaggcaggag
     6241 gacttctacc ctttcctcaa ggataaccgc gagaagatcg agaagattct gactttcagg
     6301 atcccgtact acgtcggccc actcgctagg ggcaactccc gcttcgcttg gatgacccgc
     6361 aagtcagagg agacgatcac gccgtggaac ttcgaggagg tggtcgacaa gggcgctagc
     6421 gctcagtcgt tcatcgagag gatgacgaat ttcgacaaga acctgccaaa tgagaaggtg
     6481 ctccctaagc actcgctcct gtacgagtac ttcacagtct acaacgagct gactaaggtg
     6541 aagtatgtga ccgagggcat gaggaagccg gctttcctgt ctggggagca gaagaaggcc
     6601 atcgtggacc tcctgttcaa gaccaaccgg aaggtcacgg ttaagcagct caaggaggac
     6661 tacttcaaga agattgagtg cttcgattcg gtcgagatct ctggcgttga ggaccgcttc
     6721 aacgcctccc tggggaccta ccacgatctc ctgaagatca ttaaggataa ggacttcctg
     6781 gacaacgagg agaatgagga tatcctcgag gacattgtgc tgacactcac tctgttcgag
     6841 gaccgggaga tgatcgagga gcgcctgaag acttacgccc atctcttcga tgacaaggtc
     6901 atgaagcagc tcaagaggag gaggtacacc ggctggggga ggctgagcag gaagctcatc
     6961 aacggcattc gggacaagca gtccgggaag acgatcctcg acttcctgaa gagcgatggc
     7021 ttcgcgaacc gcaatttcat gcagctgatt cacgatgaca gcctcacatt caaggaggat
     7081 atccagaagg ctcaggtgag cggccagggg gactcgctgc acgagcatat cgcgaacctc
     7141 gctggctcgc cagctatcaa gaaggggatt ctgcagaccg tgaaggttgt ggacgagctg
     7201 gtgaaggtca tgggcaggca caagcctgag aacatcgtca ttgagatggc ccgggagaat
     7261 cagaccacgc agaagggcca gaagaactca cgcgagagga tgaagaggat cgaggagggc
     7321 attaaggagc tggggtccca gatcctcaag gagcacccgg tggagaacac gcagctgcag
     7381 aatgagaagc tctacctgta ctacctccag aatggccgcg atatgtatgt ggaccaggag
     7441 ctggatatta acaggctcag cgattacgac gtcgatcata tcgttccaca gtcattcctg
     7501 aaggatgact ccattgacaa caaggtcctc accaggtcgg acaagaaccg gggcaagtct
     7561 gataatgttc cttcagagga ggtcgttaag aagatgaaga actactggcg ccagctcctg
     7621 aatgccaagc tgatcacgca gcggaagttc gataacctca caaaggctga gaggggcggg
     7681 ctctctgagc tggacaaggc gggcttcatc aagaggcagc tggtcgagac acggcagatc
     7741 actaagcacg ttgcgcagat tctcgactca cggatgaaca ctaagtacga tgagaatgac
     7801 aagctgatcc gcgaggtgaa ggtcatcacc ctgaagtcaa agctcgtctc cgacttcagg
     7861 aaggatttcc agttctacaa ggttcgggag atcaacaatt accaccatgc ccatgacgcg
     7921 tacctgaacg cggtggtcgg cacagctctg atcaagaagt acccaaagct cgagagcgag
     7981 ttcgtgtacg gggactacaa ggtttacgat gtgaggaaga tgatcgccaa gtcggagcag
     8041 gagattggca aggctaccgc caagtacttc ttctactcta acattatgaa tttcttcaag
     8101 acagagatca ctctggccaa tggcgagatc cggaagcgcc ccctcatcga gacgaacggc
     8161 gagacggggg agatcgtgtg ggacaagggc agggatttcg cgaccgtcag gaaggttctc
     8221 tccatgccac aagtgaatat cgtcaagaag acagaggtcc agactggcgg gttctctaag
     8281 gagtcaattc tgcctaagcg gaacagcgac aagctcatcg cccgcaagaa ggactgggat
     8341 ccgaagaagt acggcgggtt cgacagcccc actgtggcct actcggtcct ggttgtggcg
     8401 aaggttgaga agggcaagtc caagaagctc aagagcgtga aggagctgct ggggatcacg
     8461 attatggagc gctccagctt cgagaagaac ccgatcgatt tcctggaggc gaagggctac
     8521 aaggaggtga agaaggacct gatcattaag ctccccaagt actcactctt cgagctggag
     8581 aacggcagga agcggatgct ggcttccgct ggcgagctgc agaaggggaa cgagctggct
     8641 ctgccgtcca agtatgtgaa cttcctctac ctggcctccc actacgagaa gctcaagggc
     8701 agccccgagg acaacgagca gaagcagctg ttcgtcgagc agcacaagca ttacctcgac
     8761 gagatcattg agcagatttc cgagttctcc aagcgcgtga tcctggccga cgcgaatctg
     8821 gataaggtcc tctccgcgta caacaagcac cgcgacaagc caatcaggga gcaggctgag
     8881 aatatcattc atctcttcac cctgacgaac ctcggcgccc ctgctgcttt caagtacttc
     8941 gacacaacta tcgatcgcaa gaggtacaca agcactaagg aggtcctgga cgcgaccctc
     9001 atccaccagt cgattaccgg cctctacgag acgcgcatcg acctgtctca gctcgggggc
     9061 gacaagcggc cagcggcgac gaagaaggcg gggcaggcga agaagaagaa gacccgcgac
     9121 tccggcggca gcacgaacct ctccgacatc atcgagaagg agacgggcaa gcagctcgtg
     9181 atccaggaga gcatcctcat gctgccggag gaggtggagg aggtcatcgg caacaagccc
     9241 gagtccgaca tcctcgtgca caccgcctac gacgagtcca cggacgagaa cgtcatgctc
     9301 ctgacgagcg acgctccaga gtacaagcca tgggctctcg tgatccagga cagcaacggc
     9361 gagaacaaga tcaagatgct gtccggcggc tccccaaaga agaagcggaa ggtgtaggag
     9421 ctcagagctt tcgttcgtat catcggtttc gacaacgttc gtcaagttca atgcatcagt
     9481 ttcattgcgc acacaccaga atcctactga gtttgagtat tatggcattg ggaaaactgt
     9541 ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt ttttattcgg ttttcgctat
     9601 cgaactgtga aatggaaatg gatggagaag agttaatgaa tgatatggtc cttttgttca
     9661 ttctcaaatt aatattattt gttttttctc ttatttgttg tgtgttgaat ttgaaattat
     9721 aagagatatg caaacatttt gttttgagta aaaatgtgtc aaatcgtggc ctctaatgac
     9781 cgaagttaat atgaggagta aaacacttgt agttgtacca ttatgcttat tcactaggca
     9841 acaaatatat tttcagacct agaaaagctg caaatgttac tgaatacaag tatgtcctct
     9901 tgtgttttag acatttatga actttccttt atgtaatttt ccagaatcct tgtcagattc
     9961 taatcattgc tttataatta tagttatact catggatttg tagttgagta tgaaaatatt
    10021 ttttaatgca ttttatgact tgccaattga ttgacaacga attcgtaatc atgtcatagc
    10081 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
    10141 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
    10201 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
    10261 gcgcggggag aggcggtttg cgtattggct agagcagctt gccaacatgg tggagcacga
    10321 cactctcgtc tactccaaga atatcaaaga tacagtctca gaagaccaaa gggctattga
    10381 gacttttcaa caaagggtaa tatcgggaaa cctcctcgga ttccattgcc cagctatctg
    10441 tcacttcatc aaaaggacag tagaaaagga aggtggcacc tacaaatgcc atcattgcga
    10501 taaaggaaag gctatcgttc aagatgcctc tgccgacagt ggtcccaaag atggaccccc
    10561 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga
    10621 ttgatgtgaa catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag
    10681 tctcagaaga ccaaagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc
    10741 tcggattcca ttgcccagct atctgtcact tcatcaaaag gacagtagaa aaggaaggtg
    10801 gcacctacaa atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctgccg
    10861 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc
    10921 caaccacgtc ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg
    10981 cacaatccca ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg
    11041 agaggacacg ctgaaatcac cagtctctct ctacaaatct atctctctcg agctttcgca
    11101 gatccggggg gcaatgagat atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
    11161 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
    11221 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
    11281 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
    11341 ttccggaagt gcttgacatt ggggagttta gcgagagcct gacctattgc atctcccgcc
    11401 gttcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctacaac
    11461 cggtcgcgga ggctatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
    11521 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
    11581 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
    11641 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
    11701 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
    11761 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
    11821 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
    11881 cggagcttgc aggatcgcca cgactccggg cgtatatgct ccgcattggt cttgaccaac
    11941 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
    12001 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
    12061 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
    12121 gcactcgtcc gagggcaaag aaatagagta gatgccgacc gggatctgtc gatcgacaag
    12181 ctcgagtttc tccataataa tgtgtgagta gttcccagat aagggaatta gggttcctat
    12241 agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg tatttgtaaa
    12301 atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtacta aaatccagat
    12361 cccccgaatt aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag
    12421 ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct
    12481 ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga
    12541 cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt
    12601 cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca
    12661 tgttgattgt aacgatgaca gagcgttgct gcctgtgatc accgcggttt caaaatcggc
    12721 tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc tgttttctgg
    12781 tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct
    12841 tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga
    12901 gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag
    12961 gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa
    13021 tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc
    13081 tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct
    13141 ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg
    13201 aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca
    13261 tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt
    13321 acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat
    13381 ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg
    13441 tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg
    13501 gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg
    13561 tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac
    13621 tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt
    13681 agtacctaga atgcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    13741 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    13801 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    13861 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
    13921 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    13981 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    14041 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    14101 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    14161 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    14221 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    14281 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    14341 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    14401 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    14461 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    14521 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
    14581 tttcacaccg catatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
    14641 agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac
    14701 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
    14761 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
    14821 gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga cggcgcggct tgtccgcgcc
    14881 ctggtagatt gcctggccgt aggccagcca tttttgagcg gccagcggcc gcgataggcc
    14941 gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag ggtaggcgct ttttgcagct
    15001 cttcggctgt gcgctggcca gacagttatg cacaggccag gcgggtttta agagttttaa
    15061 taagttttaa agagttttag gcggaaaaat cgcctttttt ctcttttata tcagtcactt
    15121 acatgtgtga ccggttccca atgtacggct ttgggttccc aatgtacggg ttccggttcc
    15181 caatgtacgg ctttgggttc ccaatgtacg tgctatccac aggaaacaga ccttttcgac
    15241 ctttttcccc tgctagggca atttgcccta gcatctgctc cgtacattag gaaccggcgg
    15301 atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag gatcgggcca gcctgccccg
    15361 cctcctcctt caaatcgtac tccggcaggt catttgaccc gatcagcttg cgcacggtga
    15421 aacagaactt cttgaactct ccggcgctgc cactgcgttc gtagatcgtc ttgaacaacc
    15481 atctggcttc tgccttgcct gcggcgcggc gtgccaggcg gtagagaaaa cggccgatgc
    15541 cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac gtccgggttc ttgccttctg
    15601 tgatctcgcg gtacatccaa tcagctagct cgatctcgat gtactccggc cgcccggttt
    15661 cgctctttac gatcttgtag cggctaatca aggcttcacc ctcggatacc gtcaccaggc
    15721 ggccgttctt ggccttcttc gtacgctgca tggcaacgtg cgtggtgttt aaccgaatgc
    15781 aggtttctac caggtcgtct ttctgctttc cgccatcggc tcgccggcag aacttgagta
    15841 cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc tcccttccct tcccggtatc
    15901 ggttcatgga ttcggttaga tgggaaaccg ccatcagtac caggtcgtaa tcccacacac
    15961 tggccatgcc ggccggccct gcggaaacct ctacgtgccc gtctggaagc tcgtagcgga
    16021 tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa aacggccacg tccatgatgc
    16081 tgcgactatc gcgggtgccc acgtcataga gcatcggaac gaaaaaatct ggttgctcgt
    16141 cgcccttggg cggcttccta atcgacggcg caccggctgc cggcggttgc cgggattctt
    16201 tgcggattcg atcagcggcc gcttgccacg attcaccggg gcgtgcttct gcctcgatgc
    16261 gttgccgctg ggcggcctgc gcggccttca acttctccac caggtcatca cccagcgccg
    16321 cgccgatttg taccgggccg gatggtttgc gaccgctcac gccgattcct cgggcttggg
    16381 ggttccagtg ccattgcagg gccggcaggc aacccagccg cttacgcctg gccaaccgcc
    16441 cgttcctcca cacatggggc attccacggc gtcggtgcct ggttgttctt gattttccat
    16501 gccgcctcct ttagccgcta aaattcatct actcatttat tcatttgctc atttactctg
    16561 gtagctgcgc gatgtattca gatagcagct cggtaatggt cttgccttgg cgtaccgcgt
    16621 acatcttcag cttggtgtga tcctccgccg gcaactgaaa gttgacccgc ttcatggctg
    16681 gcgtgtctgc caggctggcc aacgttgcag ccttgctgct gcgtgcgctc ggacggccgg
    16741 cacttagcgt gtttgtgctt ttgctcattt tctctttacc tcattaactc aaatgagttt
    16801 tgatttaatt tcagcggcca gcgcctggac ctcgcgggca gcgtcgccct cgggttctga
    16861 ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag ctcacgcgct gcgtgatacg
    16921 ggactcaaga atgggcagct cgtacccggc cagcgcctcg gcaacctcac cgccgatgcg
    16981 cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt agccttccat ccgtgacctc
    17041 aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc catatgtcgt aagggcttgg
    17101 ctgcaccgga atcagcacga agtcggctgc cttgatcgcg gacacagcca agtccgccgc
    17161 ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg atggccttca cgtcgcggtc
    17221 aatcgtcggg cggtcgatgc cgacaacggt tagcggttga tcttcccgca cggccgccca
    17281 atcgcgggca ctgccctggg gatcggaatc gactaacaga acatcggccc cggcgagttg
    17341 cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct gacccgcctt tctggttaag
    17401 tacagcgata accttcatgc gttccccttg cgtatttgtt tatttactca tcgcatcata
    17461 tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct ttttagacgg
    17521 cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt ggcatcagac
    17581 aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg ctcgaacacg
    17641 tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg ttcgtcctgg
    17701 ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg gccgccaggg
    17761 cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc tcggtgggcg
    17821 tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg ccaagcagtg
    17881 cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg tgcgcgatct
    17941 gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg gcggcctcgc
    18001 gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg ccggcgaaca
    18061 cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct gccaggctac
    18121 gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg cgggtgctgc
    18181 gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg tggtcaagca
    18241 tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa aacagcttgg
    18301 tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg tcggtgctga
    18361 cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa tgtctccggt
    18421 tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa cgccaggaaa
    18481 agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg ttttcagaag
    18541 acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt tggatcaaag
    18601 tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga cgaacggata
    18661 aaccttttca cgccctttta aatatccgat tattctaa
//
