LOCUS       sequence-257666-        9283 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Endogenous tagging of GluA2: C-terminal (amino acid position: STOP 
            codon).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9283)
  AUTHORS   Willems J, de Jong APH, Scheefhals N, Mertens E, Catsburg LAE, 
            Poorthuis RB, de Winter F, Verhaagen J, Meye FJ, MacGillavry HD
  TITLE     ORANGE: A CRISPR/Cas9-based genome editing toolbox for epitope 
            tagging of endogenous proteins in neurons.
  JOURNAL   PLoS Biol. 2020 Apr 10;18(4):e3000665. doi: 
            10.1371/journal.pbio.3000665.
  PUBMED    32275651
REFERENCE   2  (bases 1 to 9283)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Biol."; date: "2020-04-10"; volume: "18(4):e3000665. doi"; pages: " 
            10.1371/journal.pbio.3000665"
COMMENT     Sequence Label: pORANGE Gria2-GFP KI
FEATURES             Location/Qualifiers
     source          1..9283
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          227..454
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
     CDS             475..501
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             505..525
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             535..4635
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             535..4635
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             4639..4659
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(4699..4716)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4705..4912
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     repeat_region   4921..5061
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4921..5050
                     /label=AAV2 ITR
     rep_origin      5136..5591
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5223..5242)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5433..5454
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5608..5627)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5727..5749
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5787..5805)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5873..5977
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5978..6046,6047..6838)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6196..6215)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7009..7597
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7498..7517
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        7659..7907
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     7659..7679
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     7830..7849
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        7929..8004
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             join(8157..8159,8160..8873)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(8157..8159,8160..8870)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(8460..8479)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8807..8828
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     enhancer        8945..9230
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     promoter        join(9232..9283,1..226)
                     /label=chicken beta-actin promoter
ORIGIN
        1 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
       61 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg
      121 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
      181 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
      241 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
      301 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta
      361 gctgagcaag aggtaagggt ttaagggatg gttggttggt ggggtattaa tgtttaatta
      421 cctggagcac ctgcctgaaa tcactttttt tcaggttgga ccggtgccac catgtaccca
      481 tacgatgttc cagattacgc ttcgccgaag aaaaagcgca aggtcgaagc gtccgacaag
      541 aagtacagca tcggcctgga catcggcacc aactctgtgg gctgggccgt gatcaccgac
      601 gagtacaagg tgcccagcaa gaaattcaag gtgctgggca acaccgaccg gcacagcatc
      661 aagaagaacc tgatcggagc cctgctgttc gacagcggcg aaacagccga ggccacccgg
      721 ctgaagagaa ccgccagaag aagatacacc agacggaaga accggatctg ctatctgcaa
      781 gagatcttca gcaacgagat ggccaaggtg gacgacagct tcttccacag actggaagag
      841 tccttcctgg tggaagagga taagaagcac gagcggcacc ccatcttcgg caacatcgtg
      901 gacgaggtgg cctaccacga gaagtacccc accatctacc acctgagaaa gaaactggtg
      961 gacagcaccg acaaggccga cctgcggctg atctatctgg ccctggccca catgatcaag
     1021 ttccggggcc acttcctgat cgagggcgac ctgaaccccg acaacagcga cgtggacaag
     1081 ctgttcatcc agctggtgca gacctacaac cagctgttcg aggaaaaccc catcaacgcc
     1141 agcggcgtgg acgccaaggc catcctgtct gccagactga gcaagagcag acggctggaa
     1201 aatctgatcg cccagctgcc cggcgagaag aagaatggcc tgttcggaaa cctgattgcc
     1261 ctgagcctgg gcctgacccc caacttcaag agcaacttcg acctggccga ggatgccaaa
     1321 ctgcagctga gcaaggacac ctacgacgac gacctggaca acctgctggc ccagatcggc
     1381 gaccagtacg ccgacctgtt tctggccgcc aagaacctgt ccgacgccat cctgctgagc
     1441 gacatcctga gagtgaacac cgagatcacc aaggcccccc tgagcgcctc tatgatcaag
     1501 agatacgacg agcaccacca ggacctgacc ctgctgaaag ctctcgtgcg gcagcagctg
     1561 cctgagaagt acaaagagat tttcttcgac cagagcaaga acggctacgc cggctacatt
     1621 gacggcggag ccagccagga agagttctac aagttcatca agcccatcct ggaaaagatg
     1681 gacggcaccg aggaactgct cgtgaagctg aacagagagg acctgctgcg gaagcagcgg
     1741 accttcgaca acggcagcat cccccaccag atccacctgg gagagctgca cgccattctg
     1801 cggcggcagg aagattttta cccattcctg aaggacaacc gggaaaagat cgagaagatc
     1861 ctgaccttcc gcatccccta ctacgtgggc cctctggcca ggggaaacag cagattcgcc
     1921 tggatgacca gaaagagcga ggaaaccatc accccctgga acttcgagga agtggtggac
     1981 aagggcgctt ccgcccagag cttcatcgag cggatgacca acttcgataa gaacctgccc
     2041 aacgagaagg tgctgcccaa gcacagcctg ctgtacgagt acttcaccgt gtataacgag
     2101 ctgaccaaag tgaaatacgt gaccgaggga atgagaaagc ccgccttcct gagcggcgag
     2161 cagaaaaagg ccatcgtgga cctgctgttc aagaccaacc ggaaagtgac cgtgaagcag
     2221 ctgaaagagg actacttcaa gaaaatcgag tgcttcgact ccgtggaaat ctccggcgtg
     2281 gaagatcggt tcaacgcctc cctgggcaca taccacgatc tgctgaaaat tatcaaggac
     2341 aaggacttcc tggacaatga ggaaaacgag gacattctgg aagatatcgt gctgaccctg
     2401 acactgtttg aggacagaga gatgatcgag gaacggctga aaacctatgc ccacctgttc
     2461 gacgacaaag tgatgaagca gctgaagcgg cggagataca ccggctgggg caggctgagc
     2521 cggaagctga tcaacggcat ccgggacaag cagtccggca agacaatcct ggatttcctg
     2581 aagtccgacg gcttcgccaa cagaaacttc atgcagctga tccacgacga cagcctgacc
     2641 tttaaagagg acatccagaa agcccaggtg tccggccagg gcgatagcct gcacgagcac
     2701 attgccaatc tggccggcag ccccgccatt aagaagggca tcctgcagac agtgaaggtg
     2761 gtggacgagc tcgtgaaagt gatgggccgg cacaagcccg agaacatcgt gatcgaaatg
     2821 gccagagaga accagaccac ccagaaggga cagaagaaca gccgcgagag aatgaagcgg
     2881 atcgaagagg gcatcaaaga gctgggcagc cagatcctga aagaacaccc cgtggaaaac
     2941 acccagctgc agaacgagaa gctgtacctg tactacctgc agaatgggcg ggatatgtac
     3001 gtggaccagg aactggacat caaccggctg tccgactacg atgtggacca tatcgtgcct
     3061 cagagctttc tgaaggacga ctccatcgac aacaaggtgc tgaccagaag cgacaagaac
     3121 cggggcaaga gcgacaacgt gccctccgaa gaggtcgtga agaagatgaa gaactactgg
     3181 cggcagctgc tgaacgccaa gctgattacc cagagaaagt tcgacaatct gaccaaggcc
     3241 gagagaggcg gcctgagcga actggataag gccggcttca tcaagagaca gctggtggaa
     3301 acccggcaga tcacaaagca cgtggcacag atcctggact cccggatgaa cactaagtac
     3361 gacgagaatg acaagctgat ccgggaagtg aaagtgatca ccctgaagtc caagctggtg
     3421 tccgatttcc ggaaggattt ccagttttac aaagtgcgcg agatcaacaa ctaccaccac
     3481 gcccacgacg cctacctgaa cgccgtcgtg ggaaccgccc tgatcaaaaa gtaccctaag
     3541 ctggaaagcg agttcgtgta cggcgactac aaggtgtacg acgtgcggaa gatgatcgcc
     3601 aagagcgagc aggaaatcgg caaggctacc gccaagtact tcttctacag caacatcatg
     3661 aactttttca agaccgagat taccctggcc aacggcgaga tccggaagcg gcctctgatc
     3721 gagacaaacg gcgaaaccgg ggagatcgtg tgggataagg gccgggattt tgccaccgtg
     3781 cggaaagtgc tgagcatgcc ccaagtgaat atcgtgaaaa agaccgaggt gcagacaggc
     3841 ggcttcagca aagagtctat cctgcccaag aggaacagcg ataagctgat cgccagaaag
     3901 aaggactggg accctaagaa gtacggcggc ttcgacagcc ccaccgtggc ctattctgtg
     3961 ctggtggtgg ccaaagtgga aaagggcaag tccaagaaac tgaagagtgt gaaagagctg
     4021 ctggggatca ccatcatgga aagaagcagc ttcgagaaga atcccatcga ctttctggaa
     4081 gccaagggct acaaagaagt gaaaaaggac ctgatcatca agctgcctaa gtactccctg
     4141 ttcgagctgg aaaacggccg gaagagaatg ctggcctctg ccggcgaact gcagaaggga
     4201 aacgaactgg ccctgccctc caaatatgtg aacttcctgt acctggccag ccactatgag
     4261 aagctgaagg gctcccccga ggataatgag cagaaacagc tgtttgtgga acagcacaag
     4321 cactacctgg acgagatcat cgagcagatc agcgagttct ccaagagagt gatcctggcc
     4381 gacgctaatc tggacaaagt gctgtccgcc tacaacaagc accgggataa gcccatcaga
     4441 gagcaggccg agaatatcat ccacctgttt accctgacca atctgggagc ccctgccgcc
     4501 ttcaagtact ttgacaccac catcgaccgg aagaggtaca ccagcaccaa agaggtgctg
     4561 gacgccaccc tgatccacca gagcatcacc ggcctgtacg agacacggat cgacctgtct
     4621 cagctgggag gcgacagccc caagaagaag agaaaggtgg aggccagcta agaattctaa
     4681 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     4741 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     4801 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     4861 ggcaggacag caagggggag gattgggaag agaatagcag gcatgctggg gagcggccgc
     4921 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     4981 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
     5041 gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg catctgtgcg
     5101 gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg gcgcattaag
     5161 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccttagcgcc
     5221 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     5281 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     5341 aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     5401 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     5461 actcaactct atctcgggct attcttttga tttataaggg attttgccga tttcggtcta
     5521 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     5581 gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca
     5641 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     5701 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     5761 atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     5821 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     5881 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     5941 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6001 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6061 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6121 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6181 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     6241 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6301 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6361 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6421 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6481 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6541 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6601 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6661 tattgctgat aaatctggag ccggtgagcg tggaagccgc ggtatcattg cagcactggg
     6721 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6781 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6841 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     6901 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     6961 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7021 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7081 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7141 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7201 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7261 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7321 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7381 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7441 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7501 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7561 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7621 acggttcctg gccttttgct ggccttttgc tcacatgtga gggcctattt cccatgattc
     7681 cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg
     7741 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
     7801 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt
     7861 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccgtc gagagtgtta
     7921 aaatttaggt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
     7981 gaaaaagtgg caccgagtcg gtgctttttt gttttagagc tagaaatagc aagttaaaat
     8041 aaggctagtc cgtttttagc gcgtgcgcca attctgcaga caaatggctc tagaagcttc
     8101 ccctaaattt taacactctc gactctgcta gcggtagcgg ctccagtaga tctggggtga
     8161 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     8221 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     8281 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     8341 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     8401 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     8461 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     8521 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     8581 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     8641 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     8701 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     8761 gcacccagtc cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     8821 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag taggcctccc
     8881 ctaaatttta acactctcga ctcgaggatc ctaggacgcg tcctaggacg cgtccagagg
     8941 tacccgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     9001 cattgacgtc aatagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     9061 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     9121 ttgacgtcaa tgacggtaaa tggcccgcct ggcattgtgc ccagtacatg accttatggg
     9181 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
     9241 gccccacgtt ctgcttcact ctccccatct cccccccctc ccc
//
