LOCUS       sequence-257677-        9362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian Expression of Flag tagged BRAF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9362)
  AUTHORS   Noeparast A, Giron P, Noor A, Bahadur Shahi R, De Brakeleer S, 
            Eggermont C, Vandenplas H, Boeckx B, Lambrechts D, De Greve J, 
            Teugels E
  TITLE     CRAF mutations in lung cancer can be oncogenic and predict 
            sensitivity to combined type II RAF and MEK inhibition.
  JOURNAL   Oncogene. 2019 Aug;38(31):5933-5941. doi: 10.1038/s41388-019-0866-7.
            Epub 2019 Jul 8.
  PUBMED    31285551
REFERENCE   2  (bases 1 to 9362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41388-019-0866-7"; journalName: "Oncogene"; date: 
            "2019-08"; volume: "38"; issue: "31"; pages: "5933-5941"
COMMENT     Sequence Label: pLenti-puro /BRAF-V600E
FEATURES             Location/Qualifiers
     source          1..9362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..181
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    228..353
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    846..1079
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1264..1308
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1890..2093
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2044..2064
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2095..2113
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2116..2134
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             4489..4554
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4570..4587
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4610..4627)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     primer_bind     complement(4639..4659)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4644..4973
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4824..4959
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4983..5582
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4983..5002)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5479..5499
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5654..5887
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5959..6080
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5996..6015)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6050..6069
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6120..6255
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6275..6294)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6276..6294)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6304..6321)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6304..6320)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6313..6335)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6462..6917
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6549..6568)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6759..6780
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6943..7047
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7048..7116,7117..7908)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7266..7285)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8079..8667
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8568..8587
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8821..8838
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8955..8976
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8991..8996,8997..9014,9015..9021)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9029..9045
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9034..9056
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9053..9069
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9053..9069
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9088..9108
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9090..9108
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9136..9362
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
ORIGIN
        1 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
       61 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
      121 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
      181 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
      241 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
      301 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
      361 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
      421 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
      481 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
      541 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
      601 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
      661 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
      721 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
      781 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
      841 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
      901 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
      961 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     1021 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     1081 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     1141 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     1201 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     1261 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     1321 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     1381 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     1441 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     1501 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     1561 cggtatcgat aagcttggga gttccgctta cataacttac ggtaaatggc ccgcctggct
     1621 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     1681 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     1741 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     1801 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     1861 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     1921 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     1981 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2041 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctctcccta
     2101 tcagtgatag agatctccct atcagtgata gagatcgtcg actagtggat ccagtgtggt
     2161 ggaattctgc agatggcggc gctgagcggt ggcggtggtg gcggcgcgga gccgggccag
     2221 gctctgttca acggggacat ggagcccgag gccggcgccg gcgccggcgc cgcggcctct
     2281 tcggctgcgg accctgccat tccggaggag gtgtggaata tcaaacaaat gattaagttg
     2341 acacaggaac atatagaggc cctattggac aaatttggtg gggagcataa tccaccatca
     2401 atatatctgg aggcctatga agaatacacc agcaagctag atgcactcca acaaagagaa
     2461 caacagttat tggaatctct ggggaacgga actgattttt ctgtttctag ctctgcatca
     2521 atggataccg ttacatcttc ttcctcttct agcctttcag tgctaccttc atctctttca
     2581 gtttttcaaa atcccacaga tgtggcacgg agcaacccca agtcaccaca aaaacctatc
     2641 gttagagtct tcctgcccaa caaacagagg acagtggtac ctgcaaggtg tggagttaca
     2701 gtccgagaca gtctaaagaa agcactgatg atgagaggtc taatcccaga gtgctgtgct
     2761 gtttacagaa ttcaggatgg agagaagaaa ccaattggtt gggacactga tatttcctgg
     2821 cttactggag aagaattgca tgtggaagtg ttggagaatg ttccacttac aacacacaac
     2881 tttgtacgaa aaacgttttt caccttagca ttttgtgact tttgtcgaaa gctgcttttc
     2941 cagggtttcc gctgtcaaac atgtggttat aaatttcacc agcgttgtag tacagaagtt
     3001 ccactgatgt gtgttaatta tgaccaactt gatttgctgt ttgtctccaa gttctttgaa
     3061 caccacccaa taccacagga agaggcgtcc ttagcagaga ctgccctaac atctggatca
     3121 tccccttccg cacccgcctc ggactctatt gggccccaaa ttctcaccag tccgtctcct
     3181 tcaaaatcca ttccaattcc acagcccttc cgaccagcag atgaagatca tcgaaatcaa
     3241 tttgggcaac gagaccgatc ctcatcagct cccaatgtgc atataaacac aatagaacct
     3301 gtcaatattg atgacttgat tagagaccaa ggatttcgtg gtgatggagg atcaaccaca
     3361 ggtttgtctg ctaccccccc tgcctcatta cctggctcac taactaacgt gaaagcctta
     3421 cagaaatctc caggacctca gcgagaaagg aagtcatctt catcctcaga agacaggaat
     3481 cgaatgaaaa cacttggtag acgggactcg agtgatgatt gggagattcc tgatgggcag
     3541 attacagtgg gacaaagaat tggatctgga tcatttggaa cagtctacaa gggaaagtgg
     3601 catggtgatg tggcagtgaa aatgttgaat gtgacagcac ctacacctca gcagttacaa
     3661 gccttcaaaa atgaagtagg agtactcagg aaaacacgac atgtgaatat cctactcttc
     3721 atgggctatt ccacaaagcc acaactggct attgttaccc agtggtgtga gggctccagc
     3781 ttgtatcacc atctccatat cattgagacc aaatttgaga tgatcaaact tatagatatt
     3841 gcacgacaga ctgcacaggg catggattac ttacacgcca agtcaatcat ccacagagac
     3901 ctcaagagta ataatatatt tcttcatgaa gacctcacag taaaaatagg tgattttggt
     3961 ctagctacag agaaatctcg atggagtggg tcccatcagt ttgaacagtt gtctggatcc
     4021 attttgtgga tggcaccaga agtcatcaga atgcaagata aaaatccata cagctttcag
     4081 tcagatgtat atgcatttgg aattgttctg tatgaattga tgactggaca gttaccttat
     4141 tcaaacatca acaacaggga ccagataatt tttatggtgg gacgaggata cctgtctcca
     4201 gatctcagta aggtacggag taactgtcca aaagccatga agagattaat ggcagagtgc
     4261 ctcaaaaaga aaagagatga gagaccactc tttccccaaa ttctcgcctc tattgagctg
     4321 ctggcccgct cattgccaaa aattcaccgc agtgcatcag aaccctcctt gaatcgggct
     4381 ggtttccaaa cagaggattt tagtctatat gcttgtgctt ctccaaaaac acccatccag
     4441 gcagggggat atggtgcgtt tcctgtccac tctagaggat cccgggctga ctacaaagac
     4501 catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga caagtagacg
     4561 cgtaccggtc atcatcacca tcaccattga gtttaaacct gctgatcagc ctcgactgtg
     4621 ccttctagtc gaccctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     4681 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     4741 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     4801 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     4861 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     4921 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta
     4981 ccatgaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg
     5041 tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg
     5101 accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg
     5161 acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg
     5221 agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg
     5281 gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg
     5341 agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg
     5401 gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc
     5461 tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg
     5521 ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct
     5581 gaggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     5641 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct
     5701 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     5761 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     5821 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     5881 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     5941 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     6001 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     6061 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     6121 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     6181 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     6241 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     6301 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     6361 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     6421 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     6481 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     6541 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     6601 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     6661 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     6721 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     6781 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     6841 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     6901 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     6961 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     7021 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     7081 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     7141 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     7201 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     7261 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     7321 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     7381 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     7441 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     7501 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     7561 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     7621 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     7681 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     7741 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     7801 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     7861 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     7921 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     7981 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     8041 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     8101 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     8161 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     8221 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     8281 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     8341 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     8401 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     8461 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     8521 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     8581 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     8641 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     8701 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     8761 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     8821 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     8881 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     8941 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     9001 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     9061 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     9121 gagctgcaag cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga
     9181 tgagttagca acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag
     9241 taaggtggta cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac
     9301 gaaccactga attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa
     9361 ac
//
