LOCUS       sequence-257695-        2894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and HaloTag-linker sequence for N-terminus tagging of 
            human UBTF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2894)
  TITLE     Allen Institute for Cell Sciences Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: AICSDP-80:UBTF-HaloTag
FEATURES             Location/Qualifiers
     source          1..2894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             998..1888
                     /codon_start=1
                     /product="modified bacterial dehalogenase that forms 
                     covalent bonds with chloroalkane derivatives"
                     /label=HaloTag(R)
                     /translation="MAEIGTGFPFDPHYVEVLGERMHYVDVGPRDGTPVLFLHGNPTSS
                     YVWRNIIPHVAPTHRCIAPDLIGMGKSDKPDLGYFFDDHVRFMDAFIEALGLEEVVLVI
                     HDWGSALGFHWAKRNPERVKGIAFMEFIRPIPTWDEWPEFARETFQAFRTTDVGRKLII
                     DQNVFIEGTLPMGVVRPLTEVEMDHYREPFLNPVDREPLWRFPNELPIAGEPANIVALV
                     EEYMDWLHQSPVPKLLFWGTPGVLIPPAEAARLAKSLPNCKAVDIGPGLNLLQEDNPDL
                     IGSEIARWLSTLEISG"
     CDS             2491..2502
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gcttctcgga gccggcaggc gcagttctcc ccggccgccg gagggggcgg cggggccggt
       61 gagcgctgcg cccccgcctc ccggccgccg tcctcccgcc ctctccttgg ctcggctccc
      121 cggaggccgg ggcgggggcg ccggcggggc tgggccgcag gctcggggga ggaggcggcg
      181 gttcccgccg cggtcgcgcc tctgtgccgg gctcctccgt gcgcgtcggg gtggctgggc
      241 ggtggcggcg cctctggccg gggtgggtgg ctcgggggcg gggggcaggc taggccgctt
      301 ctctccggct gcttgcccgc ctgtgcgggc ggggacggcg aagggagccc gggagggggg
      361 agcggcggga atccggatcc ggtccccggg ggcggtgcgg ccgctccgct ccggggctgg
      421 cggcggctgc gctgcgtccg ggtccccgcc gggttgcgga ggcgggggga accggccggg
      481 gggaggcggg aggaggagac tggggcgcgt gcccgcttcc gtggccgcgc gcgcccttta
      541 cccgcccttc ctcttccccc gccctccagc ctggggcctc cccggggcgg gcgctccgag
      601 gaggaggaga ttttttcttt tttttttttt ttggctgggg ttgggaagcg gggcggaggg
      661 cgggcgctcg ctgcacgcgg gagctaggag caggccctcg cgtggccagg ggagggcgcg
      721 cgccgcggag ccgggctgca ggggctgggg gagggggtca cgaggaagag gaggggcgca
      781 ctcccattcc catagacccc ttaagtctaa taaggtcatg gctgagtctc tcagagtgtg
      841 gacctgcccc cttctactct gggcggttag aaagtcgtta gtcgttcacc tctcagcctt
      901 cctgagtggg aactggtatg tcttccaaag ggtgaggtgg ctttgacccc gggttgcccg
      961 gccagcacga ccgaggaggt ggctggacag ctagaggatg gcagaaatcg gtactggctt
     1021 tccattcgac ccccattatg tggaagtcct gggcgagcgc atgcactacg tcgatgttgg
     1081 tccgcgcgat ggcacccctg tgctgttcct gcacggtaac ccgacctcct cctacgtgtg
     1141 gcgcaacatc atcccgcatg ttgcaccgac ccatcgctgc attgctccag acctgatcgg
     1201 tatgggcaaa tccgacaaac cagacctggg ttatttcttc gacgaccacg tccgcttcat
     1261 ggatgccttc atcgaagccc tgggtctgga agaggtcgtc ctggtcattc acgactgggg
     1321 ctccgctctg ggtttccact gggccaagcg caatccagag cgcgtcaaag gtattgcatt
     1381 tatggagttc atccgcccta tcccgacctg ggacgaatgg ccagaatttg cccgcgagac
     1441 cttccaggcc ttccgcacca ccgacgtcgg ccgcaagctg atcatcgatc agaacgtttt
     1501 tatcgagggt acgctgccga tgggtgtcgt ccgcccgctg actgaagtcg agatggacca
     1561 ttaccgcgag ccgttcctga atcctgttga ccgcgagcca ctgtggcgct tcccaaacga
     1621 gctgccaatc gccggtgagc cagcgaacat cgtcgcgctg gtcgaagaat acatggactg
     1681 gctgcaccag tcccctgtcc cgaagctgct gttctggggc accccaggcg ttctgatccc
     1741 accggccgaa gccgctcgcc tggccaaaag cctgcctaac tgcaaggctg tggacatcgg
     1801 cccgggtctg aatctgctgc aagaagacaa cccggacctg atcggcagcg agatcgcgcg
     1861 ctggctgtcg acgctcgaga tttccggctc cggcaacgga gaagccgact gccccaccga
     1921 tctggaaatg gccgccccca aaggccaagg taggggcatc cacccccggc cctctgaaac
     1981 ttaaaccctc tttccctcgt ggctgcgggg ctcactccct tcttcaaggg ctttttcagt
     2041 ctgcatacac tgaactgggg gagaaagtgc cttgagtatt tatgaatcaa gcatctccca
     2101 cacttggggt ttggctgaat ccaaattatt tggcctggag tggctcacct cccctctctc
     2161 cctgtccctc accctctgtg ttagaggagg cctgccttct ggggttccca ttcagcctga
     2221 agtgtgtccc agcccagtgt cctggaatgg gaggaaggat gagtgtagag gaagggtcag
     2281 gaaggggttg gggaaggctt ctcaggcttt ttaggaagac catgaggctt ttgtagaggc
     2341 aggtggagaa aagggctgtt ttggtggttt taggccccaa gccatgggat atatgtactc
     2401 aactagatct ctgcctcatt cagcagtgat ccagtctaga tggggataga gagtaggatg
     2461 aagctggaca gctggccatg cttgtgggac attgagggga gaggggatta tgggtccacg
     2521 tggcccattc tgcagaatct ctggcccagg ttaccagatg caaatgaaaa tggagagccc
     2581 ttttgacttc tggccttggg tggctattct cccagcccat ccacagtggc ctagagctct
     2641 ctggggctgc atttcagtgg ctgaaaaata actgttccag acctgttctt gcttccctgg
     2701 gtagttgggc tgacaagaca gcttgtaatt ctgggggttc tccctggagc agggccctga
     2761 gagtttctgt gcacctgcct accttgcctc agagctgccc tgtatcctgc ttctgcttct
     2821 gtttcctttt tcttatttgc cagaaattct cccttgatct gctttaaggg gctagggggt
     2881 ggtgggtgtc tcta
//
