LOCUS       sequence-257717-       10905 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heterologous, copper-inducible expression of (E)-alpha-bisabolene 
            synthase from Abies grandis (AgBIS) in Synechocystis sp. PCC 6803. 
            The AgBIS CDS is fused to a C-termial Flag-tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10905)
  AUTHORS   Dienst D, Wichmann J, Mantovani O, Rodrigues JS, Lindberg P
  TITLE     High density cultivation for efficient sesquiterpenoid biosynthesis 
            in Synechocystis sp. PCC 6803.
  JOURNAL   Sci Rep. 2020 Apr 3;10(1):5932. doi: 10.1038/s41598-020-62681-w.
  PUBMED    32246065
REFERENCE   2  (bases 1 to 10905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2020-04-3"; volume: "10(1):5932. doi"; pages: " 
            10.1038/s41598-020-62681-w"
COMMENT     Sequence Label: pSHDY*in_PpetE:BCD2:AgBIS:Flag
FEATURES             Location/Qualifiers
     source          1..10905
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(161..976)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(161..976)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     884..903
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     promoter        complement(977..1068)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1094..1111)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(1155..1946)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     misc_feature    2152..2173
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2176..2247
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      2263..2290
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             2804..2812
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             5288..5311
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     terminator      5332..5403
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5419..5446
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(5659..6510)
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TAECHVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDTLCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     CDS             complement(6497..7336)
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             complement(7847..8818)
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     oriT            10057..10144
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     rep_origin      10486..10880
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
ORIGIN
        1 ggtaccgagg tcgatgtcga ctaacgaccc tgccctgaac cgacgacaag ctgacgaccg
       61 ggtctccgca agtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
      121 ctaaatacat tcaaatatgt atccgctcat gaattaattc ttagaaaaac tcatcgagca
      181 tcaaatgaaa ctgcaattta ttcatatcag gattatcaat accatatttt tgaaaaagcc
      241 gtttctgtaa tgaaggagaa aactcaccga ggcagttcca taggatggca agatcctggt
      301 atcggtctgc gattccgact cgtccaacat caatacaacc tattaatttc ccctcgtcaa
      361 aaataaggtt atcaagtgag aaatcaccat gagtgacgac tgaatccggt gagaatggca
      421 aaagtttatg catttctttc cagacttgtt caacaggcca gccattacgc tcgtcatcaa
      481 aatcactcgc atcaaccaaa ccgttattca ttcgtgattg cgcctgagcg agacgaaata
      541 cgcggtcgct gttaaaagga caattacaaa caggaatcga atgcaaccgg cgcaggaaca
      601 ctgccagcgc atcaacaata ttttcacctg aatcaggata ttcttctaat acctggaatg
      661 ctgttttccc ggggatcgca gtggtgagta accatgcatc atcaggagta cggataaaat
      721 gcttgatggt cggaagaggc ataaattccg tcagccagtt tagtctgacc atctcatctg
      781 taacatcatt ggcaacgcta cctttgccat gtttcagaaa caactctggc gcatcgggct
      841 tcccatacaa tcgatagatt gtcgcacctg attgcccgac attatcgcga gcccatttat
      901 acccatataa atcagcatcc atgttggaat ttaatcgcgg cctagagcaa gacgtttccc
      961 gttgaatatg gctcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     1021 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggcatgcagc
     1081 gctcttccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactc tcgagagcgg
     1141 ccgaagctta gacattattt gccgactacc ttggtgatct cgcctttcac gtagtggaca
     1201 aattcttcca actgatctgc gcgcgaggcc aagcgatctt cttcttgtcc aagataagcc
     1261 tgtctagctt caagtatgac gggctgatac tgggccggca ggcgctccat tgcccagtcg
     1321 gcagcgacat ccttcggcgc gattttgccg gttactgcgc tgtaccaaat gcgggacaac
     1381 gtaagcacta catttcgctc atcgccagcc cagtcgggcg gcgagttcca tagcgttaag
     1441 gtttcattta gcgcctcaaa tagatcctgt tcaggaaccg gatcaaagag ttcctccgcc
     1501 gctggaccta ccaaggcaac gctatgttct cttgcttttg tcagcaagat agccagatca
     1561 atgtcgatcg tggctggctc gaagatacct gcaagaatgt cattgcgctg ccattctcca
     1621 aattgcagtt cgcgcttagc tggataacgc cacggaatga tgtcgtcgtg cacaacaatg
     1681 gtgacttcta cagcgcggag aatctcgctc tctccagggg aagccgaagt ttccaaaagg
     1741 tcgttgatca aagctcgccg cgttgtttca tcaagcctta cggtcaccgt aaccagcaaa
     1801 tcaatatcac tgtgtggctt caggccgcca tccactgcgg agccgtacaa atgtacggcc
     1861 agcaacgtcg gttcgagatg gcgctcgatg acgccaacta cctctgatag ttgagtcgat
     1921 acttcggcga tcaccgcttc cctcatgatg tttaactttg ttttagggcg actgccctgc
     1981 tgcgtaacat cgttgctgct ccataacatc aaacatcgac ccacggcgta acgcgcttgc
     2041 tgcttggatg cccgaggcat agactgtacc ccaaaaaaac agtcataaca agccatgaaa
     2101 accgccactg cgccgttacc accgctgcgt tcggtcaagg ttctggacca ggaattcgcg
     2161 gccgcttcta gagatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt
     2221 atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg
     2281 cctttctgcg tttttaccct acacttaaac cccatctcaa tacttcccat caaatccaac
     2341 ctaactcata cattccaaac taatctaact tcttccatca ttaaccacac ctctctatca
     2401 tcacctattc ccctctatct ccctcaaatt ctttcacata ttaaaaactt actcttcctc
     2461 ctttcctcct taaaatacta aataactctt aaaatagaag ggatagcaag ctaattttta
     2521 tgacggcgat cgccaaaaac aaagaaaatt cagcaattac cgtgggtagc aaaaaatccc
     2581 catctaaagt tcagtaaata tagctagaac aaccaagcat tttcggcaaa gtactattca
     2641 gatagaacga gaaatgagct tgttctatcc gcccggggct gaggctgtat aatctacgac
     2701 gggctgtcaa acattgtgat accatgggca gaagggccca agttcactta aaaaggagat
     2761 caacaatgaa agcaattttc gtactgaaac atcttaatca tgctaaggag gttttctaat
     2821 ggctagtgtg agtgccgtta gcaaagtgag ttctctggtg tgcgatctgt ctagcacttc
     2881 cgggctcatt cggcgtacag ccaaccctca tccaaatgtg tgggggtacg atctggtgca
     2941 ctctttgaaa tctccctata tcgattcttc ctatcgtgaa cgggctgaag tgctcgtttc
     3001 tgagatcaaa gccatgctga atcccgctat taccggcgac ggggaatcca tgattacccc
     3061 atccgcctat gatactgcct gggttgcccg cgttcccgca attgatggta gcgcccgtcc
     3121 ccaatttccc caaacggttg actggattct gaagaaccag ttaaaagacg gctcttgggg
     3181 aatccaatcc cactttctat tgtccgatcg gttattagcc accttgagct gcgtcttagt
     3241 attattgaag tggaacgttg gcgatctcca agtggaacag ggcattgaat ttattaaaag
     3301 taatctcgag ctagtcaagg acgaaacgga tcaggatagt ttggtgacag attttgagat
     3361 tattttcccc agtttactgc gtgaagcgca gtccctgcgg ttgggactgc cgtatgactt
     3421 accttatatc catttgttac aaacaaaacg acaagaacga ttagctaagc tgtctcgcga
     3481 ggaaatctac gctgtgccta gtcctctgtt atactcctta gaaggtattc aggatatcgt
     3541 cgaatgggaa cgtattatgg aagttcagag tcaagacgga agttttttga gcagcccagc
     3601 ctccactgct tgtgtgttta tgcataccgg tgatgccaaa tgtttagaat ttctcaattc
     3661 tgtgatgatc aaatttggca attttgtgcc atgtttatat ccagtagact tgttggaacg
     3721 gctgttgatt gttgataata tcgtccggtt ggggatttac cgtcattttg agaaggagat
     3781 taaagaagcg ctggattatg tttaccgcca ttggaatgag cgcgggattg gttggggtcg
     3841 gctaaatccc attgcggact tagaaactac cgcattgggc tttcgcttgc tacgtctgca
     3901 tcgttataat gtgtcccccg cgatttttga taactttaaa gatgccaatg gcaagtttat
     3961 ttgcagcaca gggcagttta ataaagatgt ggcgtccatg ttaaatctct accgcgcctc
     4021 tcaattagcc tttcctggtg aaaatatttt ggacgaagcc aaatcttttg cgacgaaata
     4081 cctccgcgag gcgctcgaaa agagcgaaac ctcctccgct tggaataaca aacagaatct
     4141 gtcccaagaa attaaatatg ccttaaagac ctcctggcac gcaagcgtac ctcgggttga
     4201 agctaagcgc tattgccagg tgtaccgccc tgattacgcc cgcattgcca agtgtgttta
     4261 caaactcccc tacgtcaaca acgagaaatt cttggaactc gggaaattag acttcaatat
     4321 tattcagagt attcatcaag aagaaatgaa gaatgtaacc agttggtttc gtgacagtgg
     4381 tctacccttg tttacctttg cccgagaacg tcccctagag ttttactttc tggtggcagc
     4441 gggtacctac gaaccccaat acgccaagtg ccggtttttg tttaccaaag tggcctgttt
     4501 acaaaccgtc ctcgatgaca tgtatgatac ctatggcaca ttagatgaat taaaattgtt
     4561 taccgaagcc gtccgtcggt gggacctgag ttttaccgaa aatctgcccg attacatgaa
     4621 gttatgctat caaatttatt atgatattgt gcacgaagtt gcctgggaag ccgaaaaaga
     4681 acaaggccgc gaattggtga gcttttttcg taaagggtgg gaggactatc tattgggcta
     4741 ctatgaagaa gctgagtggt tagccgccga atatgtgccc accttagatg aatatattaa
     4801 gaatggcatc accagcattg gacaacgcat tttgttgtta agcggcgttt tgattatgga
     4861 cggccaatta ctatcccaag aggctctcga aaaagtcgac taccccgggc ggcgtgttct
     4921 aactgaactc aattctttaa tttcccggtt agccgacgat accaaaacat acaaagctga
     4981 aaaagcccgt ggcgaattgg ccagcagtat tgaatgttat atgaaagatc accccgaatg
     5041 tacagaagaa gaagctctgg atcacatcta tagcatctta gaaccggccg tcaaagagct
     5101 aacccgcgaa tttttaaagc cagacgatgt gccctttgcg tgcaaaaaaa tgctcttcga
     5161 agaaacccgt gttactatgg tgattttcaa agatggtgat ggttttggag tgagcaagct
     5221 agaagttaaa gatcatatca aggagtgctt gattgaacct ttgcccctcc ggtccggtag
     5281 cggaagtgac tataaggatg atgatgacaa gggatagccg cggccaggca tcaaataaaa
     5341 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     5401 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tactgcagga
     5461 gcagaagagc atacatctgg aagcaaagcc aggaaagcgg cctatggagc tgtgcggcag
     5521 cgctcagtag gcaatttttc aaaatattgt taagcctttt ctgagcatgg tatttttcat
     5581 ggtattacca attagcagga aaataagcca ttgaatataa aagataaaaa tgtcttgttt
     5641 acaatagagt ggggggggtc agcctgccgc cttgggccgg gtgatgtcgt acttgcccgc
     5701 cgcgaactcg gttaccgtcc agcccagcgc gaccagctcc ggcaacgcct cgcgcacccg
     5761 ctggcggcgc ttgcgcatgg tcgaaccact ggcctctgac ggccagacat agccgcacaa
     5821 ggtatctatg gaagccttgc cggttttgcc ggggtcgatc cagccacaca gccgctggtg
     5881 cagcaggcgg gcggtttcgc tgtccagcgc ccgcacctcg tccatgctga tgcgcacatg
     5941 ctggccgcca cccatgacgg cctgcgcgat caaggggttc agggccacgt acaggcgccc
     6001 gtccgcctcg tcgctggcgt actccgacag cagccgaaac ccctgccgct tgcggccatt
     6061 ctgggcgatg atggatacct tccaaaggcg ctcgatgcag tcctgtatgt gcttgagcgc
     6121 cccaccacta tcgacctctg ccccgatttc ctttgccagc gcccgatagc tacctttgac
     6181 cacatggcat tcagcggtga cggcctccca cttgggttcc aggaacagcc ggagctgccg
     6241 tccgccttcg gtcttgggtt ccgggccaag cactaggcca ttaggcccag ccatggccac
     6301 cagcccttgc aggatgcgca gatcatcagc gcccagcggc tccgggccgc tgaactcgat
     6361 ccgcttgccg tcgccgtagt catacgtcac gtccagcttg ctgcgcttgc gctcgccccg
     6421 cttgagggca cggaacaggc cgggggccag acagtgcgcc gggtcgtgcc ggacgtggct
     6481 gaggctgtgc ttgttcttag gcttcaccac ggggcacccc cttgctcttg cgctgcctct
     6541 ccagcacggc gggcttgagc accccgccgt catgccgcct gaaccaccga tcagcgaacg
     6601 gtgcgccata gttggccttg ctcacaccga agcggacgaa gaaccggcgc tggtcgtcgt
     6661 ccacacccca ttcctcggcc tcggcgctgg tcatgctcga caggtaggac tgccagcgga
     6721 tgttatcgac cagtaccgag ctgccccggc tggcctgctg ctggtcgcct gcgcccatca
     6781 tggccgcgcc cttgctggca tggtgcagga acacgataga gcacccggta tcggcggcga
     6841 tggcctccat gcgaccgatg acctgggcca tggggccgct ggcgttttct tcctcgatgt
     6901 ggaaccggcg cagcgtgtcc agcaccatca ggcggcggcc ctcggcggcg cgcttgaggc
     6961 cgtcgaacca ctccggggcc atgatgttgg gcaggctgcc gatcagcggc tggatcagca
     7021 ggccgtcagc cacggcttgc cgttcctcgg cgctgaggtg cgccccaagg gcgtgcaggc
     7081 ggtgatgaat ggcggtgggc gggtcttcgg cgggcaggta gatcaccggg ccggtgggca
     7141 gttcgcccac ctccagcaga tccggcccgc ctgcaatctg tgcggccagt tgcagggcca
     7201 gcatggattt accggcacca ccgggcgaca ccagcgcccc gaccgtaccg gccaccatgt
     7261 tgggcaaaac gtagtccagc ggtggcggcg ctgctgcgaa cgcctccaga atattgatag
     7321 gcttatgggt agccattgat tgcctccttt gcaggcagtt ggtggttagg cgctggcggg
     7381 gtcactaccc ccgccctgcg ccgctctgag ttcttccagg cactcgcgca gcgcctcgta
     7441 ttcgtcgtcg gtcagccaga acttgcgctg acgcatccct ttggccttca tgcgctcggc
     7501 atatcgcgct tggcgtacag cgtcagggct ggccagcagg tcgccggtct gcttgtcctt
     7561 ttggtctttc atatcagtca ccgagaaact tgccggggcc gaaaggcttg tcttcgcgga
     7621 acaaggacaa ggtgcagccg tcaaggttaa ggctggccat atcagcgact gaaaagcggc
     7681 cagcctcggc cttgtttgac gtataaccaa agccaccggg caaccaatag cccttgtcac
     7741 ttttgatcag gtagaccgac cctgaagcgc ttttttcgta ttccataaaa cccccttctg
     7801 tgcgtgagta ctcatagtat aacaggcgtg agtaccaacg caagcactac atgctgaaat
     7861 ctggcccgcc cctgtccatg cctcgctggc ggggtgccgg tgcccgtgcc agctcggccc
     7921 gcgcaagctg gacgctgggc agacccatga ccttgctgac ggtgcgctcg atgtaatccg
     7981 cttcgtggcc gggcttgcgc tctgccagcg ctgggctggc ctcggccatg gccttgccga
     8041 tttcctcggc actgcggccc cggctggcca gcttctgcgc ggcgataaag tcgcacttgc
     8101 tgaggtcatc accgaagcgc ttgaccagcc cggccatctc gctgcggtac tcgtccagcg
     8161 ccgtgcgccg gtggcggcta agctgccgct cgggcagttc gaggctggcc agcctgcggg
     8221 ccttctcctg ctgccgctgg gcctgctcga tctgctggcc agcctgctgc accagcgccg
     8281 ggccagcggt ggcggtcttg cccttggatt cacgcagcag cacccacggc tgataaccgg
     8341 cgcgggtggt gtgcttgtcc ttgcggttgg tgaagcccgc caagcggcca tagtggcggc
     8401 tgtcggcgct ggccgggtcg gcgtcgtact cgctggccag cgtccgggca atctgccccc
     8461 gaagttcacc gcctgcggcg tcggccacct tgacccatgc ctgatagttc ttcgggctgg
     8521 tttccactac cagggcaggc tcccggccct cggctttcat gtcatccagg tcaaactcgc
     8581 tgaggtcgtc caccagcacc agaccatgcc gctcctgctc ggcgggcctg atatacacgt
     8641 cattgccctg ggcattcatc cgcttgagcc atggcgtgtt ctggagcact tcggcggctg
     8701 accattcccg gttcatcatc tggccggtgg tggcgtccct gacgccgata tcgaagcgct
     8761 cacagcccat ggccttgagc tgtcggccta tggcctgcaa agtcctgtcg ttcttcatcg
     8821 ggccaccaag cgcagccaga tcgagccgtc ctcggttgtc agtggcgtca ggtcgagcaa
     8881 gagcaacgat gcgatcagca gcaccaccgt aggcatcatg gaagccagca tcacggttag
     8941 ccatagcttc cagtgccacc cccgcgacgc gctccgggcg ctctgcgcgg cgctgctcac
     9001 ctcggcggct acctcccgca actctttggc cagctccacc catgccgccc ctgtctggcg
     9061 ctgggctttc agccactccg ccgcctgcgc ctcgctggcc tgctgggtct ggctcatgac
     9121 ctgccgggct tcgtcggcca gtgtcgccat gctctgggcc agcggttcga tctgctccgc
     9181 taactcgttg atgcctctgg atttcttcac tctgtcgatt gcgttcatgg tctattgcct
     9241 cccggtattc ctgtaagtcg atgatctggg cgttggcggt gtcgatgttc agggccacgt
     9301 ctgcccggtc ggtgcggatg ccccggcctt ccatctccac cacgttcggc cccaggtgaa
     9361 caccgggcag gcgctcgatg ccctgcgcct caagtgttct gtggtcaatg cgggcgtcgt
     9421 ggccagcccg ctctaatgcc cggttggcat ggtcggccca tgcctcgcgg gtctgctcaa
     9481 gccatgcctt gggcttgagc gcttcggtct tctgtgcccc gcccttctcc ggggtcttgc
     9541 cgttgtaccg cttgaaccac tgagcggcgg gccgctcgat gccgtcattg atccgctcgg
     9601 agatcatcag gtggcagtgc gggttctcgc cgccaccggc atggatggcc agcgtatacg
     9661 gcaggcgctc ggcaccggtc aggtgctggg cgaactcgga cgccagcgcc ttctgctggt
     9721 cgagggtcag ctcgaccggc agggcaaatt cgacctcctt gaacagccgc ccattggcgc
     9781 gttcatacag gtcggcagca tcccagtagt cggcgggccg ctcgacgaac tccggcatgt
     9841 gcccggattc ggcgtgcaag acttcatcca tgtcgcgggc atacttgcct tcgcgctgga
     9901 tgtagtcggc cttggccctg gccgattggc cgcccgacct gctgccggtt ttcgccgtaa
     9961 ggtgataaat cgccatgctg cctcgctgtt gcttttgctt ttcggctcca tgcaatggcc
    10021 ctcggagagc gcaccgcccg aagggtggcc gttaggccag tttctcgaag agaaaccggt
    10081 aagtgcgccc tcccctacaa agtagggtcg ggattgccgc cgctgtgcct ccatgatagc
    10141 ctacgagaca gcacattaac aatggggtgt caagatggtt aaggggagca acaaggcggc
    10201 ggatcggctg gccaagctcg aagaacaacg agcgcgaatc aatgccgaaa ttcagcgggt
    10261 gcgggcaagg gaacagcagc aagagcgcaa gaacgaaaca aggcgcaagg tgctggtggg
    10321 ggccatgatt ttggccaagg tgaacagcag cgagtggccg gaggatcggc tcatggcggc
    10381 aatggatgcg taccttgaac gcgaccacga ccgcgccttg ttcggtctgc cgccacgcca
    10441 gaaggatgag ccgggctgaa tgatcgaccg agacaggccc tgcggggctg cacacgcgcc
    10501 cccacccttc gggtaggggg aaaggccgct aaagcggcta aaagcgctcc agcgtatttc
    10561 tgcggggttt ggtgtggggt ttagcgggct ttgcccgcct ttccccctgc cgcgcagcgg
    10621 tggggcggtg tgtagcctag cgcagcgaat agaccagcta tccggcctct ggccgggcat
    10681 attgggcaag ggcagcagcg ccccacaagg gcgctgataa ccgcgcctag tggattattc
    10741 ttagataatc atggatggat ttttccaaca ccccgccagc ccccgcccct gctgggtttg
    10801 caggtttggg ggcgtgacag ttattgcagg ggttcgtgac agttattgca ggggggcgtg
    10861 acagttattg caggggttcg tgacagttag tacgggagtg acggg
//
