LOCUS       sequence-257725-       15482 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a functional NeoR only in females, a functional PuroR only
            in males, and dsRed in both genders..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15482)
  AUTHORS   Kandul NP, Liu J, Hsu AD, Hay BA, Akbari OS
  TITLE     A drug-inducible sex-separation technique for insects.
  JOURNAL   Nat Commun. 2020 Apr 30;11(1):2106. doi: 10.1038/s41467-020-16020-2.
  PUBMED    32355156
REFERENCE   2  (bases 1 to 15482)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-04-30"; volume: "11(1):2106. doi"; pages: " 
            10.1038/s41467-020-16020-2"
COMMENT     Sequence Label: Opie2-NeoR[traF]+Opie2-PuroR[dsxM]
FEATURES             Location/Qualifiers
     source          1..15482
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(95..683)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(175..194)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(854..1645,1646..1714))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1477..1496
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1715..1819)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1887..1905
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(1943..1965)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2065..2084
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2278..2300
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2292..2309
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2293..2309
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   3013..3075
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    4111..4245
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(4263..4859)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4346..4366)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     enhancer        10501..10983
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        10987..11578
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     CDS             join(11589..11591,11592..11594,11595..12269)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(11772..11792)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     11990..12009
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     12184..12207
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     polyA_signal    12390..12511
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             13337..14128
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    14145..14279
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   14960..14994
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(15175..15191)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(15175..15191)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(15188..15210)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    15199..15215
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(15223..15229,15230..15247,15248..15253))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    15268..15289
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(15406..15423)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
       61 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      121 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      181 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      241 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      301 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      361 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
      421 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
      481 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
      541 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
      601 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
      661 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
      721 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
      781 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
      841 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
      901 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
      961 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     1021 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     1081 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     1141 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     1201 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     1261 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     1321 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     1381 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     1441 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     1501 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     1561 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     1621 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     1681 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     1741 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     1801 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     1861 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     1921 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     1981 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     2041 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     2101 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     2161 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     2221 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     2281 agtcacgacg ttgtaaaacg acggccagtg ccaagctttg tttaaaaata taacaaaatt
     2341 gtgatcccac aaaatgaagt ggggcaaaat caaataatta atagtgtccg taaacttgtt
     2401 ggtcttcaac tttttgagga acacgttgga cggcaaatcc gtgactataa cacaagttga
     2461 tttaataatt ttagccaaca cgtcgggctg cgtgtttttt gccgacgcgt ctgtgtacac
     2521 gttgattaac tggtcgatta aactgttgaa ataatttaat ttttggttct tctttaaatc
     2581 tgtgatgaaa ttttttaaaa taactttaaa ttcttcattg gtaaaaaatg ccacgttttg
     2641 caacttgtga gggtctaata tgaggtcaaa ctcagtagga gttttatcca aaaaagaaaa
     2701 catgattacg tctgtacacg aacgcgtatt aacgcagagt gcaaagtata agagggttaa
     2761 aaaatatatt ttacgcacca tatacgcatc gggttgatat cgttaatatg gatcaatttg
     2821 aacagttgat taacgtgtct ctgctcaagt ctttgatcaa aacgcaaatc gacgaaaatg
     2881 tgtcggacaa tatcaagtcg atgagcgaaa aactaaaaag gctagaatac gacaatctca
     2941 cagacagcgt tgagatatac ggtattcacg acagcaggct gaataataaa aaaattagaa
     3001 actattattt aaccctagaa agataatcat attgtgacgt acgttaaaga taatcatgcg
     3061 taaaattgac gcatgtgttt tatcggtctg tatatcgagg tttatttatt aatttgaata
     3121 gatattaagt tttattatat ttacacttac atactaataa taaattcaac aaacaattta
     3181 tttatgttta tttatttatt aaaaaaaaac aaaaactcaa aatttcttct ataaagtaac
     3241 aaaactttta aacattctct cttttacaaa aataaactta ttttgtactt taaaaacagt
     3301 catgttgtat tataaaataa gtaattagct taacttatac ataatagaaa caaattatac
     3361 ttattagtca gtcagaaaca actttggcac atatcaatat tatgctctcg acaaataact
     3421 tttttgcatt ttttgcacga tgcatttgcc tttcgcctta ttttagaggg gcagtaagta
     3481 cagtaagtac gttttttcat tactggctct tcagtactgt catctgatgt accaggcact
     3541 tcatttggca aaatattaga gatattatcg cgcaaatatc tcttcaaagt aggagcttct
     3601 aaacgcttac gcataaacga tgacgtcagg ctcatgtaaa ggtttctcat aaattttttg
     3661 cgactttgaa ccttttctcc cttgctactg acattatggc tgtatataat aaaagaattt
     3721 atgcaggcaa tgtttatcat tccgtacaat aatgccatag gccacctatt cgtcttccta
     3781 ctgcaggtca tcacagaaca catttggtct agcgtgtcca ctccgccttt agtttgatta
     3841 taatacataa ccatttgcgg tttaccggta ctttcgttga tagaagcatc ctcatcacaa
     3901 gatgataata agtataccat cttagctggc ttcggtttat atgagacgag agtaaggggt
     3961 ccgtcaaaac aaaacatcga tgttcccact ggcctggagc gactgttttt cagtacttcc
     4021 ggtatctcgc gtttgtttga tcgcacggtt cccacaatgg ttaattcgag ctcgcccggg
     4081 gtttatttaa atcgggcccc ccctcgacat gatccagaca tgataagata cattgatgag
     4141 tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat
     4201 gctattgctt tatttgtaac cattataagc tgcaataaac aagtttcgag gtcgaagatc
     4261 tctcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg
     4321 tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc
     4381 tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg
     4441 ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag gaaccacgcg
     4501 ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg
     4561 gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg
     4621 ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg
     4681 atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg
     4741 cggtccggat cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg
     4801 cgtacggccc tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc
     4861 tggggattaa cagaagttat gcgattaaaa cttggcagat gtattttttg actccaaaat
     4921 aatggtaagc atctgaaaac ttgtgaaatt ttcgggatta cttatactta tcgttataca
     4981 cagtacacaa agcacaaagg aacactaata ctaatactaa ttatacgagt ttttattatt
     5041 tctatattta gacgaaaaaa ggtaataata aattcgttct tttcttaatg tcatctactt
     5101 ttatagtata caagattcgt ttaatttaaa agattggaaa aagtttataa tttttctcct
     5161 aactgatttc gcatctggta gttgaggacc tcaattacag cgttctctct ttttcgtgct
     5221 taattaaata tagctttaca aatgagaaca aaaaataaaa gggttcactt tccggaaaac
     5281 accttaataa cttaaacata atccagtgat tgcttgacgg tataccacat cgttaattta
     5341 acacactttt tatggaaaat agagcagagc agtttaaagt tgtggctggc ggccatggca
     5401 ttaactatgg ccgcacttaa tgtctatgcc acccgaccaa gatgtgcgcc attttggaag
     5461 gcacatgagg aacctggggt cccagttccc caggcccgaa ctccaagcac gggcccacct
     5521 atggccccgg aatgcacttt atgtgcaacc ttcgcacggt gctcacataa taaaccctcc
     5581 gctgcttcct ccaaagtgcc acgcccccag atcgacgacc acatcccgct tccagtccca
     5641 gttggagctt cggctggcta gagcctttgt cttacgctga ctccccgcct tatctgcgtg
     5701 gcaaaaactt ttcaaaattt tactgcttgc tgcaacatca aaatgccaag tttttacgat
     5761 tacgctgctg ctgctgctgc aattttactt aattttaatg ggattttttc ttccgccaac
     5821 tgcagaatgc attaaacagg gccagagcat acgagagaga attacgcatg agcgtgcctc
     5881 ccctttgtgg cccccttcct tgggttgaag agttccctcc tcggaggacg cacaagtatc
     5941 gaagtggaag gagtgaaatc ggtggagttg gagtcgaagg tacagtccat gccagaccca
     6001 gacccagacc cagacccaac ctagtcgcaa ctaatgccgc ccgcccattg tgcgatttga
     6061 cgaaataata aaaagtcgta aaaagttgtg acccaggttg ctgatggatg gagcgggtcg
     6121 gggtaaaagg agcaagttaa gctgccctcc agaccgggca ttctggtact tgttaatata
     6181 taggcaactc tcattgggtt tgtccataaa ccgagtcttg atgttggggg taattagagg
     6241 aatgcatttt cgaacaggtt tggcttggcc aggaagcgaa gtgacgacct ctcaatccga
     6301 ttcctgactt ggtgtaatga aataagtcaa gtgaaaatgg acatggtatt ttcactgttg
     6361 ttgcagcagg aaggaaatca ttcttaagcc ataagaagtt cttcaagttg gtcggaatat
     6421 tggttaaata tgtgcccgtt gtccaccaat tagttactgt taactgtatt gttcaaagtc
     6481 tacgctatga tttgattgca ttgggtaaaa ttttactggt ttttcaatgt tattaaaata
     6541 gtagtaaact atttgatgac ctcgtttaat ccgtaatctt tctaagcctt cgtttaaaga
     6601 aactagtttt gcttgctcca tccaaaggtc tataatctat gcttttgcag gccacaaaac
     6661 ctcgacaata agtttccagt cgaactgtca agacagtcca gtccagtaca gcccgattgc
     6721 gctggaatcc tttatgacaa actgcttgct tattatttag cgagccagga ccgtggccat
     6781 gatgaatcta tattgtccta aggaggccat ggctatgcat gtggatcaga tgtctggcac
     6841 aagtggcagc ttgtaagtcg tggggcagta aaaatgacag tagcgaaact agatgggaaa
     6901 cggggagcgg tcgcgaagtg gagtggagtg caagttgacg ctgcacaaat attttacgat
     6961 ccgcccacaa tatgaagtcg taaagttgga aatttttgct tcctgctgcc gctcggtctg
     7021 cgctttccta tagtttttaa taagcattgg taaatgtttg ggatgaaagt ggcaaggaat
     7081 ttgttctaga ctcgttgagc cgagccgagc tgagctgagc tgctgggttg ggccgggctg
     7141 ggctgggcag ccacatatga ggacgttgac gatgatgatg atgatacggc ccgcagctcg
     7201 ttatggcgcc ccgtacaaaa tggtatacta gcgagtctat tttttatggg aaaacaaaga
     7261 aaggcgtcgc tggaaatatg acaacgcagg gatgaggcag tgcgtcgtaa aattgggtcg
     7321 aggagagcgg ttggattcca tatgaattta ctatactata tgctctgagt cataagatga
     7381 tttgctcatc acactatttg aaaacagtat attaagatcc agttatctac atatatccat
     7441 agagagttat tttaaagatt aatggtttat tatctaatta caaaagtgag gtaaattcat
     7501 tgatcgtaaa tgaatgtatg tataaatgtt tcaagaaatt taaagtcctg ccttaaaaat
     7561 atgattatga aatatgaaat cgttatgaaa cctagttatc cttatgaatg ggaagaacat
     7621 gatatgtatc atgtcatttt gtttattgct taattattaa atattgtcca agaagctttt
     7681 aacttggact gttttagagg gttcttgtat agaaaaggta ggttaaccaa atttagtttt
     7741 taacggacct tgaatattgc aataaaactt tcgattcgtt ggcatcaaat accttaaccg
     7801 ctcggcgatt cctttctctc tctctctctt tcacttgcgc acatttcaac cataaacact
     7861 tttaagcttt atggtggcca catttacacg tcgccattgc ctacccacgg taacgcagag
     7921 attctccctc ccagatcgcc ggcgatacct gaaatttcat cgtgacgtgt ttagtgccga
     7981 ggagcgatgt cgagctgtta cggccggagc attgcagcta cctggtggcg gactagagtc
     8041 gtttaaccca gctggagaaa ctaaatccga atctgcgaat ctcaggcgta cactttgagt
     8101 aatccatatc catattaatc catattactg atatactagt tactatatta aaaaaaaata
     8161 tttacaattc taaggggagt ctagtaaact acaacttata aatggatgcg atttaaactg
     8221 aaggtgaaca caaaatcgaa gatgaattca ctgaatgcat ttctcgcagt gcaggccttt
     8281 ggcaatggtg ttggcagtgt cgtaaatttt tcgcatgcat ttttcggttt aatgatgcat
     8341 ttacgatccg agcagcagtg gcgacacaag ttactttcag ctcccagatc ccacttccca
     8401 catccttcat cccggctccc aactccaact cggcaaaact tcagctgatg atgctgggac
     8461 gtgcaatggt aatggattcg agaaatggca acaacttgaa aagcaatcgt taccagttta
     8521 atgtcatcaa caaaacaaac aacagctgat acttccagtt cagtgattca attcgcatat
     8581 ccgagggttt atgtttcagt ttcagattca gtttccaatt catcatctca tctcatcatc
     8641 tcagcgaggc cgattttgtt taaggaaaat agtttatttc tgacatttca ggtctatcct
     8701 ttttataata ttccgttggg ctaaatctta cttaaggtcg taacaatatt ttacaatgtc
     8761 aataagaatt ttcgttgtaa tgcaatatta caattagagt ttcgcaagac atcgatgaaa
     8821 cactaactta aatatatttg taatttgtgc actatctcac aacagttttg atacccagac
     8881 ccccggaaag tagttatcat ttcttcaagg gttactatgc aattgccttg ctaagataca
     8941 aattcacacg caaagaagaa atttgagatt ggcttgtatg cctattcgaa tgagtgtgta
     9001 tatcctagtt ttcttctcga tcccctttgt tgatcaattt agtgctgctt acgaaagaaa
     9061 cattattttg tacttagatt cagttaagta cttaagtaaa caataaaagc ataaatgact
     9121 gaagtgaggc gctttcaatg gctttaatta gactgtgatt agcccaataa actgagtaat
     9181 gccaaactta aataaggtta aactaaagct atacaaccga tgcgcagatt tgtgatgcga
     9241 acgtgttgaa atgttgatcg ttgatttggc gtgcgtattt cgaatatttt ttaaaaacgt
     9301 ttactacatt ttgtcctttg tcctttgatt atattattcg ttggcggcaa gggctatgtt
     9361 gattgttgat tagatccgtt tgcggattgt tgattgaaga gaagagaatg ttgattgtag
     9421 attgagatta ttaacattga ggccagacat ctcagcacgg ggtaatgttg attgaagaaa
     9481 gctgacgcgt agaacggctg tcacacatcg acatatttgg cttgttgatt gaagatagtc
     9541 caagtcgcgc tttagtttac cgataaattc gatttattta cagaggtttt gctccataaa
     9601 actctaagtt actcaactct aagttataaa agttaatttc gcgcgatttc accgaagcat
     9661 taagctaata tgtgatgctg aacaataata aatatcgggg caaagtagta ttcgttactc
     9721 tagttaatga aaaattatca tccacattgc cgcgttgtgt tgcgcagctc acccccgtca
     9781 tagatattca aattatgttg acgggagtac tcattcacaa cgtattggcc tggtttagat
     9841 cagatcgaga gacattcggc attttattat acaatttcac ttcacagcca gttgattggc
     9901 ttggcagtgg agtgatcact gaatcactga tcaacagact taccatcacc agagacaggt
     9961 tgcggcggcg gttggatggc gtgggcgcgt tggcgttgtt ggaccggctc atgttgtgtc
    10021 gctgtaacag atgctgttca actgtgttta ccagatcgtt gcgggctgta tttataggcg
    10081 cgataagcgg gacgggcgcc tcgtgtccgg tcacgcgcat gagataacgc gcggctgata
    10141 tggaggcgcg tcctgttccg ataaggagtt gcgtccggct gcggttagca acacaggaag
    10201 ctggcgtcct gtcacgataa gacaacactc gtccggtccg ataatgtgat tcgtacgtga
    10261 caggacgcga cccgataagg ccggcctacg tgactgccga cacgtacttt tttgcactgc
    10321 aaaaaggttc aatgtgtggt agtgtatttg gagcgtatac aacggtgtag actatttatg
    10381 taaaatagtc tacgaaacgt agagtttgta ctatgtatgg gcccgcgtgc aaaagcgtgt
    10441 ttttttgcag tgcaaaaaag ttggtggtgg ggaggccacc gagtatgggc gcgtttaaac
    10501 cgcgtaaaac acaatcaagt atgagtcata agctgatgtc atgttttgca cacggctcat
    10561 aaccgaactg gctttacgag tagaattcta cttgtaacgc acgatcagtg gatgatgtca
    10621 tttgtttttc aaatcgagat gatgtcatgt tttgcacacg gctcataaac tcgctttacg
    10681 agtagaattc tacgtgtaac gcacgatcga ttgatgagtc atttgttttg caatatgata
    10741 tcatacaata tgactcattt gtttttcaaa accgaacttg atttacgggt agaattctac
    10801 ttgtaaagca caatcaaaaa gatgatgtca tttgtttttc aaaactgaac tcgctttacg
    10861 agtagaattc tacgtgtaaa acacaatcaa gaaatgatgt catttgttat aaaaataaaa
    10921 gctgatgtca tgttttgcac atggctcata actaaactcg ctttacgggt agaattctac
    10981 gcgcgtcgat gtctttgtga tgcgcgcgac atttttgtag gttattgata aaatgaacgg
    11041 atacgttgcc cgacattatc attaaatcct tggcgtagaa tttgtcgggt ccattgtccg
    11101 tgtgcgctag catgcccgta acggacctcg tacttttggc ttcaaaggtt ttgcgcacag
    11161 acaaaatgtg ccacacttgc agctctgcat gtgtgcgcgt taccacaaat cccaacggcg
    11221 cagtgtactt gttgtatgca aataaatctc gataaaggcg cggcgcgcga atgcagctga
    11281 tcacgtacgc tcctcgtgtt ccgttcaagg acggtgttat cgacctcaga ttaatgttta
    11341 tcggccgact gttttcgtat ccgctcacca aacgcgtttt tgcattaaca ttgtatgtcg
    11401 gcggatgttc tatatctaat ttgaataaat aaacgataac cgcgttggtt ttagagggca
    11461 taataaaaga aatattgtta tcgtgttcgc cattagggca gtataaattg acgttcatgt
    11521 tggatattgt ttcagttgca agttgacact ggcggcgaca agatcgtgaa caaccaagtg
    11581 acctcgagat ggtgcgctcc tccaagaacg tcatcaagga gttcatgcgc ttcaaggtgc
    11641 gcatggaggg caccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
    11701 cctacgaggg ccacaacacc gtgaagctga aggtgaccaa gggcggcccc ctgcccttcg
    11761 cctgggacat cctgtccccc cagttccagt acggctccaa ggtgtacgtg aagcaccccg
    11821 ccgacatccc cgactacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
    11881 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggct
    11941 gcttcatcta caaggtgaag ttcatcggcg tgaacttccc ctccgacggc cccgtaatgc
    12001 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
    12061 tgaagggcga gatccacaag gccctgaagc tgaaggacgg cggccactac ctggtggagt
    12121 tcaagtccat ctacatggcc aagaagcccg tgcagctgcc cggctactac tacgtggact
    12181 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggagcag tacgagcgca
    12241 ccgagggccg ccaccacctg ttcctgtagc ggccgcgact ctagatcata atcagccata
    12301 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
    12361 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
    12421 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
    12481 gtggtttgtc caaactcatc aatgtatctt aaagcttatc gatacgcgta cggcgcgccc
    12541 gcgcccatac tcggtggcct ccccaccacc aacttttttg cactgcaaaa aaacacgctt
    12601 ttgcacgcgg gcccatacat agtacaaact ctacgtttcg tagactattt tacataaata
    12661 gtctacaccg ttgtatacgc tccaaataca ctaccacaca ttgaaccttt ttgcagtgca
    12721 aaaaagtacg tgtcggcagt cacgtaggcc ggccttatcg ggtcgcgtcc tgtcacgtac
    12781 gaatcacatt atcggaccgg acgagtgttg tcttatcgtg acaggacgcc agcttcctgt
    12841 gttgctaacc gcagccggac gcaactcctt atcggaacag gacgcgcctc catatcagcc
    12901 gcgcgttatc tcatgcgcgt gaccggacac gaggcgcccg tcccgcttat cgcgcctata
    12961 aatacagccc gcaacgatct ggtaaacaca gttgaacagc atctgttaca gcgacacaac
    13021 atgagccggt ccaacaacgc caacgcgccc acgccatcca accgccgccg caacctgtct
    13081 ctggtgatgg tacccactat atagtataac cgatctgatc agattctgat ccagtgccat
    13141 ctttttgttg tttttttcta gtgtcatatt gtgtgaaatg tgaaatggat gagaatgaac
    13201 gctggacgac cagacagaga aagagaagct aggacaatag gactctcaac tgcgcattac
    13261 gtggattccg tctccgacga tgcgccaaac actatgcgtt agatgcattg actaaccgca
    13321 cactcttttc acatagattg aacaagacgg actgcacgcc ggatctcctg ccgcctgggt
    13381 ggaacgcctc ttcggttacg attgggctca gcaaactatt ggttgctcgg acgccgctgt
    13441 gttccgtctg agtgcccagg ggaggccggt gttgttcgtc aagaccgacc tgtctggcgc
    13501 cctgaacgag ctgcaagatg aagccgctcg tttgtcatgg ctggctacca ctggagtgcc
    13561 ttgcgccgct gtcctggacg tggtcactga ggccggcagg gattggctgc tcttgggtga
    13621 agtgccggga caggacctgc tctccagcca cctggctcct gctgagaagg tctcgatcat
    13681 ggccgatgct atgcgccgtc tccatacgtt ggaccccgcc acatgcccat tcgatcacca
    13741 agctaagcat cgcatcgaga gggcccgcac gcgcatggaa gctggactcg tggaccagga
    13801 cgatttggat gaggaacacc aagggctggc tcccgctgag ctgttcgctc gtctcaaggc
    13861 ttccatgccc gacggagagg acctggtggt cacccacggc gacgcttgcc tccccaacat
    13921 catggtggag aatggccgct tcagcggttt cattgattgc ggacgcctgg gagtcgctga
    13981 ccgttaccaa gatatcgccc tggctacccg cgacattgcc gaggaactgg gcggcgagtg
    14041 ggctgatcgc ttcctcgtgt tgtacggcat cgccgctcca gactctcaaa ggattgcctt
    14101 ctaccgcttg ctggatgagt tcttctaaag atcttcgacc tcgaaacttg tttattgcag
    14161 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    14221 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca
    14281 tgtcgagggg gggcccgcct aggccggccg atctcggatc tgacaatgtt cagtgcagag
    14341 actcggctac gcctcgtgga ctttgaagtt gaccaacaat gtttattctt acctctaata
    14401 gtcctctgtg gcaaggtcaa gattctgtta gaagccaatg aagaacctgg ttgttcaata
    14461 acattttgtt cgtctaatat ttcactaccg cttgacgttg gctgcacttc atgtacctca
    14521 tctataaacg cttcttctgt atcgctctgg acgtcatctt cacttacgtg atctgatatt
    14581 tcactgtcag aatcctcacc aacaagctcg tcatcgcttt gcagaagagc agagaggata
    14641 tgctcatcgt ctaaagaact acccatttta ttatatatta gtcacgatat ctataacaag
    14701 aaaatatata tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg
    14761 tatgagttaa atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg
    14821 tcgttatagt tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc
    14881 caagcggcga ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga
    14941 gagcaatatt tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaaaa
    15001 aagatttgcg ctttactcga cctaaacttt aaacacgtca tagaatcttc gtttgacaaa
    15061 aaccacattg tggccaagct gtgtgacgcg acgcgcgcta aagaatggca aaccaagtcg
    15121 cgcgagcgtc gactctagag gatccccggg taccgagctc gaattcgtaa tcatgtcata
    15181 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
    15241 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
    15301 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
    15361 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
    15421 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
    15481 gt
//
