LOCUS       sequence-257743-       13722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a functional PuroR only in males and dsRed in both 
            genders..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13722)
  AUTHORS   Kandul NP, Liu J, Hsu AD, Hay BA, Akbari OS
  TITLE     A drug-inducible sex-separation technique for insects.
  JOURNAL   Nat Commun. 2020 Apr 30;11(1):2106. doi: 10.1038/s41467-020-16020-2.
  PUBMED    32355156
REFERENCE   2  (bases 1 to 13722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-04-30"; volume: "11(1):2106. doi"; pages: " 
            10.1038/s41467-020-16020-2"
COMMENT     Sequence Label: Opie2-PuroR[dsxM]
FEATURES             Location/Qualifiers
     source          1..13722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     60..77
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    194..215
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(230..235,236..253,254..260)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    268..284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     273..295
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     292..308
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     292..308
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   489..523
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    1212..1333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(1454..2128,2129..2131,2132..2134))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(1516..1539)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1714..1733)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1931..1951
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     promoter        complement(2145..2736)
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     enhancer        2740..3222
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     CDS             8864..9460
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     9357..9377
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9478..9612
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   10648..10710
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(11414..11431)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11414..11430)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11423..11445)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(11639..11658)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     11758..11780
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(11818..11836)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11904..12008
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12009..12077,12078..12869)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12227..12246)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      13040..13628
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13529..13548
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
       61 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
      121 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      181 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
      241 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
      301 gctatgacat gattacgaat tcgagctcgg tacccgggga tcctctagag tcgacgctcg
      361 cgcgacttgg tttgccattc tttagcgcgc gtcgcgtcac acagcttggc cacaatgtgg
      421 tttttgtcaa acgaagattc tatgacgtgt ttaaagttta ggtcgagtaa agcgcaaatc
      481 ttttttaacc ctagaaagat agtctgcgta aaattgacgc atgcattctt gaaatattgc
      541 tctctctttc taaatagcgc gaatccgtcg ctgtgcattt aggacatctc agtcgccgct
      601 tggagctccc gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt gaactataac
      661 gaccgcgtga gtcaaaatga cgcatgatta tcttttacgt gacttttaag atttaactca
      721 tacgataatt atattgttat ttcatgttct acttacgtga taacttatta tatatatatt
      781 ttcttgttat agatatcgtg actaatatat aataaaatgg gtagttcttt agacgatgag
      841 catatcctct ctgctcttct gcaaagcgat gacgagcttg ttggtgagga ttctgacagt
      901 gaaatatcag atcacgtaag tgaagatgac gtccagagcg atacagaaga agcgtttata
      961 gatgaggtac atgaagtgca gccaacgtca agcggtagtg aaatattaga cgaacaaaat
     1021 gttattgaac aaccaggttc ttcattggct tctaacagaa tcttgacctt gccacagagg
     1081 actattagag gtaagaataa acattgttgg tcaacttcaa agtccacgag gcgtagccga
     1141 gtctctgcac tgaacattgt cagatccgag atcggccggc ctaggcgcgc cgtacgcgta
     1201 tcgataagct ttaagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     1261 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     1321 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     1381 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct
     1441 agagtcgcgg ccgctacagg aacaggtggt ggcggccctc ggtgcgctcg tactgctcca
     1501 cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggagtccacg tagtagtagc
     1561 cgggcagctg cacgggcttc ttggccatgt agatggactt gaactccacc aggtagtggc
     1621 cgccgtcctt cagcttcagg gccttgtgga tctcgccctt cagcacgccg tcgcgggggt
     1681 acaggcgctc ggtggaggcc tcccagccca tggtcttctt ctgcattacg gggccgtcgg
     1741 aggggaagtt cacgccgatg aacttcacct tgtagatgaa gcagccgtcc tgcagggagg
     1801 agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc tcccacttga
     1861 agccctcggg gaaggacagc ttcttgtagt cggggatgtc ggcggggtgc ttcacgtaca
     1921 ccttggagcc gtactggaac tggggggaca ggatgtccca ggcgaagggc agggggccgc
     1981 ccttggtcac cttcagcttc acggtgttgt ggccctcgta ggggcggccc tcgccctcgc
     2041 cctcgatctc gaactcgtgg ccgttcacgg tgccctccat gcgcaccttg aagcgcatga
     2101 actccttgat gacgttcttg gaggagcgca ccatctcgag gtcacttggt tgttcacgat
     2161 cttgtcgccg ccagtgtcaa cttgcaactg aaacaatatc caacatgaac gtcaatttat
     2221 actgccctaa tggcgaacac gataacaata tttcttttat tatgccctct aaaaccaacg
     2281 cggttatcgt ttatttattc aaattagata tagaacatcc gccgacatac aatgttaatg
     2341 caaaaacgcg tttggtgagc ggatacgaaa acagtcggcc gataaacatt aatctgaggt
     2401 cgataacacc gtccttgaac ggaacacgag gagcgtacgt gatcagctgc attcgcgcgc
     2461 cgcgccttta tcgagattta tttgcataca acaagtacac tgcgccgttg ggatttgtgg
     2521 taacgcgcac acatgcagag ctgcaagtgt ggcacatttt gtctgtgcgc aaaacctttg
     2581 aagccaaaag tacgaggtcc gttacgggca tgctagcgca cacggacaat ggacccgaca
     2641 aattctacgc caaggattta atgataatgt cgggcaacgt atccgttcat tttatcaata
     2701 acctacaaaa atgtcgcgcg catcacaaag acatcgacgc gcgtagaatt ctacccgtaa
     2761 agcgagttta gttatgagcc atgtgcaaaa catgacatca gcttttattt ttataacaaa
     2821 tgacatcatt tcttgattgt gttttacacg tagaattcta ctcgtaaagc gagttcagtt
     2881 ttgaaaaaca aatgacatca tctttttgat tgtgctttac aagtagaatt ctacccgtaa
     2941 atcaagttcg gttttgaaaa acaaatgagt catattgtat gatatcatat tgcaaaacaa
     3001 atgactcatc aatcgatcgt gcgttacacg tagaattcta ctcgtaaagc gagtttatga
     3061 gccgtgtgca aaacatgaca tcatctcgat ttgaaaaaca aatgacatca tccactgatc
     3121 gtgcgttaca agtagaattc tactcgtaaa gccagttcgg ttatgagccg tgtgcaaaac
     3181 atgacatcag cttatgactc atacttgatt gtgttttacg cggtttaaac gcgcccatac
     3241 tcggtggcct ccccaccacc aacttttttg cactgcaaaa aaacacgctt ttgcacgcgg
     3301 gcccatacat agtacaaact ctacgtttcg tagactattt tacataaata gtctacaccg
     3361 ttgtatacgc tccaaataca ctaccacaca ttgaaccttt ttgcagtgca aaaaagtacg
     3421 tgtcggcagt cacgtaggcc ggccttatcg ggtcgcgtcc tgtcacgtac gaatcacatt
     3481 atcggaccgg acgagtgttg tcttatcgtg acaggacgcc agcttcctgt gttgctaacc
     3541 gcagccggac gcaactcctt atcggaacag gacgcgcctc catatcagcc gcgcgttatc
     3601 tcatgcgcgt gaccggacac gaggcgcccg tcccgcttat cgcgcctata aatacagccc
     3661 gcaacgatct ggtaaacaca gttgaacagc atctgttaca gcgacacaac atgagccggt
     3721 ccaacaacgc caacgcgccc acgccatcca accgccgccg caacctgtct ctggtgatgg
     3781 taagtctgtt gatcagtgat tcagtgatca ctccactgcc aagccaatca actggctgtg
     3841 aagtgaaatt gtataataaa atgccgaatg tctctcgatc tgatctaaac caggccaata
     3901 cgttgtgaat gagtactccc gtcaacataa tttgaatatc tatgacgggg gtgagctgcg
     3961 caacacaacg cggcaatgtg gatgataatt tttcattaac tagagtaacg aatactactt
     4021 tgccccgata tttattattg ttcagcatca catattagct taatgcttcg gtgaaatcgc
     4081 gcgaaattaa cttttataac ttagagttga gtaacttaga gttttatgga gcaaaacctc
     4141 tgtaaataaa tcgaatttat cggtaaacta aagcgcgact tggactatct tcaatcaaca
     4201 agccaaatat gtcgatgtgt gacagccgtt ctacgcgtca gctttcttca atcaacatta
     4261 ccccgtgctg agatgtctgg cctcaatgtt aataatctca atctacaatc aacattctct
     4321 tctcttcaat caacaatccg caaacggatc taatcaacaa tcaacatagc ccttgccgcc
     4381 aacgaataat ataatcaaag gacaaaggac aaaatgtagt aaacgttttt aaaaaatatt
     4441 cgaaatacgc acgccaaatc aacgatcaac atttcaacac gttcgcatca caaatctgcg
     4501 catcggttgt atagctttag tttaacctta tttaagtttg gcattactca gtttattggg
     4561 ctaatcacag tctaattaaa gccattgaaa gcgcctcact tcagtcattt atgcttttat
     4621 tgtttactta agtacttaac tgaatctaag tacaaaataa tgtttctttc gtaagcagca
     4681 ctaaattgat caacaaaggg gatcgagaag aaaactagga tatacacact cattcgaata
     4741 ggcatacaag ccaatctcaa atttcttctt tgcgtgtgaa tttgtatctt agcaaggcaa
     4801 ttgcatagta acccttgaag aaatgataac tactttccgg gggtctgggt atcaaaactg
     4861 ttgtgagata gtgcacaaat tacaaatata tttaagttag tgtttcatcg atgtcttgcg
     4921 aaactctaat tgtaatattg cattacaacg aaaattctta ttgacattgt aaaatattgt
     4981 tacgacctta agtaagattt agcccaacgg aatattataa aaaggataga cctgaaatgt
     5041 cagaaataaa ctattttcct taaacaaaat cggcctcgct gagatgatga gatgagatga
     5101 tgaattggaa actgaatctg aaactgaaac ataaaccctc ggatatgcga attgaatcac
     5161 tgaactggaa gtatcagctg ttgtttgttt tgttgatgac attaaactgg taacgattgc
     5221 ttttcaagtt gttgccattt ctcgaatcca ttaccattgc acgtcccagc atcatcagct
     5281 gaagttttgc cgagttggag ttgggagccg ggatgaagga tgtgggaagt gggatctggg
     5341 agctgaaagt aacttgtgtc gccactgctg ctcggatcgt aaatgcatca ttaaaccgaa
     5401 aaatgcatgc gaaaaattta cgacactgcc aacaccattg ccaaaggcct gcactgcgag
     5461 aaatgcattc agtgaattca tcttcgattt tgtgttcacc ttcagtttaa atcgcatcca
     5521 tttataagtt gtagtttact agactcccct tagaattgta aatatttttt tttaatatag
     5581 taactagtat atcagtaata tggattaata tggatatgga ttactcaaag tgtacgcctg
     5641 agattcgcag attcggattt agtttctcca gctgggttaa acgactctag tccgccacca
     5701 ggtagctgca atgctccggc cgtaacagct cgacatcgct cctcggcact aaacacgtca
     5761 cgatgaaatt tcaggtatcg ccggcgatct gggagggaga atctctgcgt taccgtgggt
     5821 aggcaatggc gacgtgtaaa tgtggccacc ataaagctta aaagtgttta tggttgaaat
     5881 gtgcgcaagt gaaagagaga gagagagaaa ggaatcgccg agcggttaag gtatttgatg
     5941 ccaacgaatc gaaagtttta ttgcaatatt caaggtccgt taaaaactaa atttggttaa
     6001 cctacctttt ctatacaaga accctctaaa acagtccaag ttaaaagctt cttggacaat
     6061 atttaataat taagcaataa acaaaatgac atgatacata tcatgttctt cccattcata
     6121 aggataacta ggtttcataa cgatttcata tttcataatc atatttttaa ggcaggactt
     6181 taaatttctt gaaacattta tacatacatt catttacgat caatgaattt acctcacttt
     6241 tgtaattaga taataaacca ttaatcttta aaataactct ctatggatat atgtagataa
     6301 ctggatctta atatactgtt ttcaaatagt gtgatgagca aatcatctta tgactcagag
     6361 catatagtat agtaaattca tatggaatcc aaccgctctc ctcgacccaa ttttacgacg
     6421 cactgcctca tccctgcgtt gtcatatttc cagcgacgcc tttctttgtt ttcccataaa
     6481 aaatagactc gctagtatac cattttgtac ggggcgccat aacgagctgc gggccgtatc
     6541 atcatcatca tcgtcaacgt cctcatatgt ggctgcccag cccagcccgg cccaacccag
     6601 cagctcagct cagctcggct cggctcaacg agtctagaac aaattccttg ccactttcat
     6661 cccaaacatt taccaatgct tattaaaaac tataggaaag cgcagaccga gcggcagcag
     6721 gaagcaaaaa tttccaactt tacgacttca tattgtgggc ggatcgtaaa atatttgtgc
     6781 agcgtcaact tgcactccac tccacttcgc gaccgctccc cgtttcccat ctagtttcgc
     6841 tactgtcatt tttactgccc cacgacttac aagctgccac ttgtgccaga catctgatcc
     6901 acatgcatag ccatggcctc cttaggacaa tatagattca tcatggccac ggtcctggct
     6961 cgctaaataa taagcaagca gtttgtcata aaggattcca gcgcaatcgg gctgtactgg
     7021 actggactgt cttgacagtt cgactggaaa cttattgtcg aggttttgtg gcctgcaaaa
     7081 gcatagatta tagacctttg gatggagcaa gcaaaactag tttctttaaa cgaaggctta
     7141 gaaagattac ggattaaacg aggtcatcaa atagtttact actattttaa taacattgaa
     7201 aaaccagtaa aattttaccc aatgcaatca aatcatagcg tagactttga acaatacagt
     7261 taacagtaac taattggtgg acaacgggca catatttaac caatattccg accaacttga
     7321 agaacttctt atggcttaag aatgatttcc ttcctgctgc aacaacagtg aaaataccat
     7381 gtccattttc acttgactta tttcattaca ccaagtcagg aatcggattg agaggtcgtc
     7441 acttcgcttc ctggccaagc caaacctgtt cgaaaatgca ttcctctaat tacccccaac
     7501 atcaagactc ggtttatgga caaacccaat gagagttgcc tatatattaa caagtaccag
     7561 aatgcccggt ctggagggca gcttaacttg ctccttttac cccgacccgc tccatccatc
     7621 agcaacctgg gtcacaactt tttacgactt tttattattt cgtcaaatcg cacaatgggc
     7681 gggcggcatt agttgcgact aggttgggtc tgggtctggg tctgggtctg gcatggactg
     7741 taccttcgac tccaactcca ccgatttcac tccttccact tcgatacttg tgcgtcctcc
     7801 gaggagggaa ctcttcaacc caaggaaggg ggccacaaag gggaggcacg ctcatgcgta
     7861 attctctctc gtatgctctg gccctgttta atgcattctg cagttggcgg aagaaaaaat
     7921 cccattaaaa ttaagtaaaa ttgcagcagc agcagcagcg taatcgtaaa aacttggcat
     7981 tttgatgttg cagcaagcag taaaattttg aaaagttttt gccacgcaga taaggcgggg
     8041 agtcagcgta agacaaaggc tctagccagc cgaagctcca actgggactg gaagcgggat
     8101 gtggtcgtcg atctgggggc gtggcacttt ggaggaagca gcggagggtt tattatgtga
     8161 gcaccgtgcg aaggttgcac ataaagtgca ttccggggcc ataggtgggc ccgtgcttgg
     8221 agttcgggcc tggggaactg ggaccccagg ttcctcatgt gccttccaaa atggcgcaca
     8281 tcttggtcgg gtggcataga cattaagtgc ggccatagtt aatgccatgg ccgccagcca
     8341 caactttaaa ctgctctgct ctattttcca taaaaagtgt gttaaattaa cgatgtggta
     8401 taccgtcaag caatcactgg attatgttta agttattaag gtgttttccg gaaagtgaac
     8461 ccttttattt tttgttctca tttgtaaagc tatatttaat taagcacgaa aaagagagaa
     8521 cgctgtaatt gaggtcctca actaccagat gcgaaatcag ttaggagaaa aattataaac
     8581 tttttccaat cttttaaatt aaacgaatct tgtatactat aaaagtagat gacattaaga
     8641 aaagaacgaa tttattatta ccttttttcg tctaaatata gaaataataa aaactcgtat
     8701 aattagtatt agtattagtg ttcctttgtg ctttgtgtac tgtgtataac gataagtata
     8761 agtaatcccg aaaatttcac aagttttcag atgcttacca ttattttgga gtcaaaaaat
     8821 acatctgcca agttttaatc gcataacttc tgttaatccc cagaccgagt acaagcccac
     8881 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     8941 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     9001 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     9061 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     9121 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     9181 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     9241 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     9301 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     9361 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     9421 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gagatcttcg acctcgaaac
     9481 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     9541 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     9601 catgtctgga tcatgtcgag ggggggcccg atttaaataa accccgggcg agctcgaatt
     9661 aaccattgtg ggaaccgtgc gatcaaacaa acgcgagata ccggaagtac tgaaaaacag
     9721 tcgctccagg ccagtgggaa catcgatgtt ttgttttgac ggacccctta ctctcgtctc
     9781 atataaaccg aagccagcta agatggtata cttattatca tcttgtgatg aggatgcttc
     9841 tatcaacgaa agtaccggta aaccgcaaat ggttatgtat tataatcaaa ctaaaggcgg
     9901 agtggacacg ctagaccaaa tgtgttctgt gatgacctgc agtaggaaga cgaataggtg
     9961 gcctatggca ttattgtacg gaatgataaa cattgcctgc ataaattctt ttattatata
    10021 cagccataat gtcagtagca agggagaaaa ggttcaaagt cgcaaaaaat ttatgagaaa
    10081 cctttacatg agcctgacgt catcgtttat gcgtaagcgt ttagaagctc ctactttgaa
    10141 gagatatttg cgcgataata tctctaatat tttgccaaat gaagtgcctg gtacatcaga
    10201 tgacagtact gaagagccag taatgaaaaa acgtacttac tgtacttact gcccctctaa
    10261 aataaggcga aaggcaaatg catcgtgcaa aaaatgcaaa aaagttattt gtcgagagca
    10321 taatattgat atgtgccaaa gttgtttctg actgactaat aagtataatt tgtttctatt
    10381 atgtataagt taagctaatt acttatttta taatacaaca tgactgtttt taaagtacaa
    10441 aataagttta tttttgtaaa agagagaatg tttaaaagtt ttgttacttt atagaagaaa
    10501 ttttgagttt ttgttttttt ttaataaata aataaacata aataaattgt ttgttgaatt
    10561 tattattagt atgtaagtgt aaatataata aaacttaata tctattcaaa ttaataaata
    10621 aacctcgata tacagaccga taaaacacat gcgtcaattt tacgcatgat tatctttaac
    10681 gtacgtcaca atatgattat ctttctaggg ttaaataata gtttctaatt tttttattat
    10741 tcagcctgct gtcgtgaata ccgtatatct caacgctgtc tgtgagattg tcgtattcta
    10801 gcctttttag tttttcgctc atcgacttga tattgtccga cacattttcg tcgatttgcg
    10861 ttttgatcaa agacttgagc agagacacgt taatcaactg ttcaaattga tccatattaa
    10921 cgatatcaac ccgatgcgta tatggtgcgt aaaatatatt ttttaaccct cttatacttt
    10981 gcactctgcg ttaatacgcg ttcgtgtaca gacgtaatca tgttttcttt tttggataaa
    11041 actcctactg agtttgacct catattagac cctcacaagt tgcaaaacgt ggcatttttt
    11101 accaatgaag aatttaaagt tattttaaaa aatttcatca cagatttaaa gaagaaccaa
    11161 aaattaaatt atttcaacag tttaatcgac cagttaatca acgtgtacac agacgcgtcg
    11221 gcaaaaaaca cgcagcccga cgtgttggct aaaattatta aatcaacttg tgttatagtc
    11281 acggatttgc cgtccaacgt gttcctcaaa aagttgaaga ccaacaagtt tacggacact
    11341 attaattatt tgattttgcc ccacttcatt ttgtgggatc acaattttgt tatattttta
    11401 aacaaagctt ggcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
    11461 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
    11521 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg cgcctgatgc
    11581 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta
    11641 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
    11701 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
    11761 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
    11821 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
    11881 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
    11941 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    12001 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    12061 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    12121 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    12181 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    12241 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    12301 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    12361 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    12421 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    12481 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    12541 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    12601 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    12661 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    12721 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    12781 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    12841 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    12901 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    12961 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    13021 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    13081 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    13141 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    13201 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    13261 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    13321 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    13381 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    13441 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    13501 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    13561 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    13621 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    13681 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga ta
//
