LOCUS       sequence-257758-       11827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ubiquitously expresses 3xHA-GFP-TOM5 in the outer mitochondrial 
            membrane of planta.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11827)
  AUTHORS   Kuhnert F, Stefanski A, Overbeck N, Drews L, Reichert AS, Stuhler K,
            Weber APM
  TITLE     Rapid Single-Step Affinity Purification of HA-Tagged Plant 
            Mitochondria.
  JOURNAL   Plant Physiol. 2020 Feb;182(2):692-706. doi: 10.1104/pp.19.00732. 
            Epub 2019 Dec 9.
  PUBMED    31818904
REFERENCE   2  (bases 1 to 11827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1104/pp.19.00732"; journalName: "Plant Physiol"; date: 
            "2020-02"; volume: "182"; issue: "2"; pages: "692-706"
COMMENT     Sequence Label: pUTKan-3xHA-GFP-TOM5
FEATURES             Location/Qualifiers
     source          1..11827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1279..1303
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    1468..1489
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1504..1509,1510..1527,1528..1534)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1542..1558
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1547..1569
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1566..1582
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1566..1582
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2273..2362
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             join(2381..2383,2384..3094)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2423..2444)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2684..2703)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3031..3052
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      3293..3934
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(3942..3959)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3942..3958)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3951..3973)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      complement(4540..4792)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     CDS             complement(4988..5782)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5099..5118)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5709..5728
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5812..6192)
                     /label=MAS promoter
                     /note="mannopine synthase promoter (Velten et al., 1984)"
     misc_feature    6564..6588
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7109..7900
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8146..8734
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8635..8654
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8888..8905
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8920..9060
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8986..9005)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9146..9168
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9404..9598
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9664..10737)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11166..11795)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata cacatcacct
       61 ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg ccgccagctt
      121 ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt gcgcgggcgg
      181 ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa tgaaaaacgg
      241 ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc attctcggcg
      301 gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg ccgcctggcc
      361 tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg
      421 ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg
      481 tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg
      541 gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg
      601 ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct
      661 gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg
      721 cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg
      781 tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa
      841 aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg
      901 tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa
      961 tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa
     1021 cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg
     1081 ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt
     1141 tggatcaaag tactttaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca
     1201 tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat tattctaata
     1261 aacgctcttt tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa
     1321 ctgaaggcgg gaaacgacaa tctgatccaa gctcaagctg ctctagccaa tacgcaaacc
     1381 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     1441 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     1501 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     1561 tcacacagga aacagctatg acatgattac gaattcgagc tcggtacccg acgagtcagt
     1621 aataaacggc gtcaaagtgg ttgcagccgg cacacacgag tcgtgtttat caactcaaag
     1681 cacaaatact tttcctcaac ctaaaaataa ggcaattagc caaaaacaac tttgcgtgta
     1741 aacaacgctc aatacacgtg tcattttatt attagctatt gcttcaccgc cttagctttc
     1801 tcgtgaccta gtcgtcctcg tcttttcttc ttcttcttct ataaaacaat acccaaagag
     1861 ctcttcttct tcacaattca gatttcaatt tctcaaaatc ttaaaaactt tctctcaatt
     1921 ctctctaccg tgatcaaggt aaatttctgt gttccttatt ctctcaaaat cttcgatttt
     1981 gttttcgttc gatcccaatt tcgtatatgt tctttggttt agattctgtt aatcttagat
     2041 cgaagacgat tttctgggtt tgatcgttag atatcatctt aattctcgat tagggtttca
     2101 tagatatcat ccgatttgtt caaataattt gagttttgtc gaataattac tcttcgattt
     2161 gtgatttcta tctagatctg gtgttagttt ctagtttgtg cgatcgaatt tgtagattaa
     2221 tctgagtttt tctgattaac actcgagtgc gggatcctct agagggccca tgtacccata
     2281 cgatgttcct gactatgcgg gctatcccta tgacgtcccg gactatgcag gatcctatcc
     2341 atatgacgtt ccagattacg ctccgcgggg agggagcggc gtgagcaagg gcgaggagct
     2401 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     2461 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     2521 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcacctacgg
     2581 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     2641 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     2701 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     2761 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     2821 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     2881 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     2941 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
     3001 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     3061 cgggatcact cacggcatgg acgagctgta caagggaggg agcggcgtcg acgtgaacaa
     3121 cgttgtctct atcgaaaaga tgaaagcact ctggcactcc gaggttcatg atgaacaaaa
     3181 atgggcggtg aacatgaaac ttctgcgagc acttggtatg tttgcaggag gagtcgtcct
     3241 catgcgtagc tatggggatc tcatgggagt ttgatgaact agttggctgc agagctttcg
     3301 ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca
     3361 caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca
     3421 tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat
     3481 ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat
     3541 attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa
     3601 acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg
     3661 aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt
     3721 cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca
     3781 tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt
     3841 ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt
     3901 tatgacttgc caattgattg acaacatgca tcaagcttgg cactggccgt cgttttacaa
     3961 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4021 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4081 agcctgaatg gcgaatgcta gagcagcttg ccaatcgagg ccgaccaacc gcaagcgttg
     4141 tcagtgttgc aaagcgctct gtgtgggcct actttaattg cttccagtgt taaattggcg
     4201 aaaggcaata atatcgcaaa atattgtgtt gtaaaatgta attatgtttt aatttcatgg
     4261 aaatgtttga gcataatttt tattaatgta ctaaattact gttttgttaa atgcaatttt
     4321 gctttctcgg gattttaata tcaaaatcta tttagaaata cacaatattt tgttgcaggc
     4381 ttgctggaga atcgatctgc tatcataaaa attacaaaaa aattttattt gcctcaatta
     4441 ttttaggatt ggtattaagg acgcttaaat tatttgtcgg gtcactacgc atcattgtga
     4501 ttgagaagat cagcgatacg aaatattcgt agtactatcg ataatttatt tgaaaattca
     4561 taagaaaagc aaacgttaca tgaattgatg aaacaataca aagacagata aagccacgca
     4621 catttaggat attggccgag attactgaat attgagtaag atcacggaat ttctgacagg
     4681 agcatgtctt caattcagcc caaatggcag ttgaaatact caaaccgccc catatgcagg
     4741 agcggatcat tcattgtttg tttggttgcc tttgccaaca tgggagtcca aggtttcagg
     4801 gaagctggaa ttccggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc
     4861 atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg
     4921 gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatttc gaaccccaga
     4981 gtcccgctca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc gaatcgggag
     5041 cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc tcttcagcaa
     5101 tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc cggccacagt
     5161 cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag gcatcgccat
     5221 gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg aacagttcgg
     5281 ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga ccggcttcca
     5341 tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg caggtagccg
     5401 gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc tcggcaggag
     5461 caaggtgaga tgacaggaga tcctgccccg gcacttcgcc caatagcagc cagtcccttc
     5521 ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg gccagccacg
     5581 atagccgcgc tgcctcgtcc tggagttcat tcagggcacc ggacaggtcg gtcttgacaa
     5641 aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag cagccgattg
     5701 tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga gaacctgcgt
     5761 gcaatccatc ttgttcaatc atgcgaaacg atccggggaa ttcgctagag tcgatttggt
     5821 gtatcgagat tggttatgaa attcagatgc tagtgtaatg tattggtaat ttgggaagat
     5881 ataataggaa gcaaggctat ttatccattt ctgaaaaggc gaaatggcgt caccgcgagc
     5941 gtcacgcgca ttccgttctt gctgtaaagc gttgtttggt acacttttga ctagcgaggc
     6001 ttggcgtgtc agcgtatcta ttcaaaagtc gttaatggct gcggatcaag aaaaagttgg
     6061 aatagaaaca gaatacccgc gaaattcagg cccggttgcc atgtcctaca cgccgaaata
     6121 aacgaccaaa ttagtagaaa aataaaaact gactcggata cttacgtcac gtcttgcgca
     6181 ctgatttgaa aaatctcaat ataaacaaag acggccacaa gaaaaaacca aaacaccgat
     6241 attcattaat cttatctagt ttctcaaaaa aattcatatc ttccacacgt gaaaatgcca
     6301 atttctcaga cctacctcgg ctctgcgaag gcccccgctg gtatcaaaag tttttatttc
     6361 atccgacatg gcgcgaccga cctcaacgag aaggaaattg tcgtgaacgg tgagaagctc
     6421 tggggcgtgc aaggttccgg aacgaacatc ggtctcaatg caaaagggga acgccaggct
     6481 ctgttggccc ctcgaaattc ggcgttaatt cagtacatta aaaacgtccg caatgtgtta
     6541 ttaagttgtc taagcgtcaa tttgtttaca ccacaatata tcctgccacc agccagccaa
     6601 cagctccccg accggcagct cggcacaaaa tcaccactcg atacaggcag cccatcagtc
     6661 cgggacggcg tcagcgggag agccgttgta aggcggcaga ctttgctcat gttaccgatg
     6721 ctattcggaa gaacggcaac taagctgccg ggtttgaaac acggatgatc tcgcggaggg
     6781 tagcatgttg attgtaacga tgacagagcg ttgctgcctg tgatcaattc gggcacgaac
     6841 ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg
     6901 caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat
     6961 ggcttgttat gactgttttt ttggggtaca gtctatgcct cgggcatcca agcagcaagc
     7021 gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc aacgatgtta cgcagcaggg
     7081 cagtcgccct aaaacaaagt taaacatcat gggggaagcg gtgatcgccg aagtatcgac
     7141 tcaactatca gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt
     7201 acatttgtac ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct
     7261 ggttacggtg accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt
     7321 ggaaacttcg gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt
     7381 tgtgcacgac gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga
     7441 atggcagcgc aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct
     7501 ggctatcttg ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga
     7561 ggaactcttt gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac
     7621 gctatggaac tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc
     7681 ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg
     7741 ggcaatggag cgcctgccgg cccagtatca gcccgtcata cttgaagcta gacaggctta
     7801 tcttggacaa gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca
     7861 ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa tgtctagcta gaaattcgtt
     7921 caagccgacg ccgcttcgcg gcgcggctta actcaagcgt tagatgcact aagcacataa
     7981 ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc gtgcataata
     8041 agccctacac aaattgggag atatatcatg catgaccaaa atcccttaac gtgagttttc
     8101 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     8161 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     8221 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     8281 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     8341 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8401 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8461 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8521 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8581 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8641 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8701 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     8761 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     8821 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     8881 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     8941 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     9001 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     9061 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     9121 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca
     9181 tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga gtggcgacgg
     9241 cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt ttgagcggcc
     9301 agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg accgaagggt
     9361 aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac aggccaggcg
     9421 ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc cttttttctc
     9481 ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg ggttcccaat
     9541 gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc tatccacagg
     9601 aaagagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca tctgctccgt
     9661 acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca tgactaggat
     9721 cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat ttgacccgat
     9781 cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac tgcgttcgta
     9841 gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg ccaggcggta
     9901 gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg tcagcacgtc
     9961 cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga tctcgatgta
    10021 ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg cttcaccctc
    10081 ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg caacgtgcgt
    10141 ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc catcggctcg
    10201 ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg gcttgtctcc
    10261 cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca tcagtaccag
    10321 gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta cgtgcccgtc
    10381 tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca gacggaaaac
    10441 ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca tcggaacgaa
    10501 aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac cggctgccgg
    10561 cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt caccggggcg
    10621 tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact tctccaccag
    10681 gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac cgctcacgcc
    10741 gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcagacaac ccagccgctt
    10801 acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc ggtgcctggt
    10861 tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact catttattca
    10921 tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg taatggtctt
    10981 gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca actgaaagtt
    11041 gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct tgctgctgcg
    11101 tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct ctttacctca
    11161 ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc gcgggcagcg
    11221 tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc tgggtagctc
    11281 acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag cgcctcggca
    11341 acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc cgcttgtagc
    11401 cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc ggtggcccat
    11461 atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt gatcgcggac
    11521 acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg ccggccgatg
    11581 gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag cggttgatct
    11641 tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac taacagaaca
    11701 tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt cttgcctgac
    11761 ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt atttgtttat
    11821 ttactca
//
