LOCUS       sequence-257770-        9080 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses a functional NeoR only in females and dsRed in both 
            genders..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Kandul NP, Liu J, Hsu AD, Hay BA, Akbari OS
  TITLE     A drug-inducible sex-separation technique for insects.
  JOURNAL   Nat Commun. 2020 Apr 30;11(1):2106. doi: 10.1038/s41467-020-16020-2.
  PUBMED    32355156
REFERENCE   2  (bases 1 to 9080)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-04-30"; volume: "11(1):2106. doi"; pages: " 
            10.1038/s41467-020-16020-2"
COMMENT     Sequence Label: Opie2-NeoR[TraF]
FEATURES             Location/Qualifiers
     source          1..9080
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     60..77
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    194..215
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(230..235,236..253,254..260)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    268..284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     273..295
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     292..308
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     292..308
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   489..523
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    1212..1333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(1454..2128,2129..2131,2132..2134))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(1516..1539)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1714..1733)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1931..1951
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     promoter        complement(2145..2736)
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     enhancer        2740..3222
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     CDS             4028..4819
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             4028..4819
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4079..4098)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4689..4708
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4836..4970
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   6006..6068
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6772..6789)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6772..6788)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6781..6803)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6997..7016)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7116..7138
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7176..7194)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7262..7366
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7367..7435,7436..8227)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7585..7604)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8398..8986
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8887..8906
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
       61 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
      121 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      181 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
      241 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
      301 gctatgacat gattacgaat tcgagctcgg tacccgggga tcctctagag tcgacgctcg
      361 cgcgacttgg tttgccattc tttagcgcgc gtcgcgtcac acagcttggc cacaatgtgg
      421 tttttgtcaa acgaagattc tatgacgtgt ttaaagttta ggtcgagtaa agcgcaaatc
      481 ttttttaacc ctagaaagat agtctgcgta aaattgacgc atgcattctt gaaatattgc
      541 tctctctttc taaatagcgc gaatccgtcg ctgtgcattt aggacatctc agtcgccgct
      601 tggagctccc gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt gaactataac
      661 gaccgcgtga gtcaaaatga cgcatgatta tcttttacgt gacttttaag atttaactca
      721 tacgataatt atattgttat ttcatgttct acttacgtga taacttatta tatatatatt
      781 ttcttgttat agatatcgtg actaatatat aataaaatgg gtagttcttt agacgatgag
      841 catatcctct ctgctcttct gcaaagcgat gacgagcttg ttggtgagga ttctgacagt
      901 gaaatatcag atcacgtaag tgaagatgac gtccagagcg atacagaaga agcgtttata
      961 gatgaggtac atgaagtgca gccaacgtca agcggtagtg aaatattaga cgaacaaaat
     1021 gttattgaac aaccaggttc ttcattggct tctaacagaa tcttgacctt gccacagagg
     1081 actattagag gtaagaataa acattgttgg tcaacttcaa agtccacgag gcgtagccga
     1141 gtctctgcac tgaacattgt cagatccgag atcggccggc ctaggcgcgc cgtacgcgta
     1201 tcgataagct ttaagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     1261 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     1321 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     1381 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct
     1441 agagtcgcgg ccgctacagg aacaggtggt ggcggccctc ggtgcgctcg tactgctcca
     1501 cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggagtccacg tagtagtagc
     1561 cgggcagctg cacgggcttc ttggccatgt agatggactt gaactccacc aggtagtggc
     1621 cgccgtcctt cagcttcagg gccttgtgga tctcgccctt cagcacgccg tcgcgggggt
     1681 acaggcgctc ggtggaggcc tcccagccca tggtcttctt ctgcattacg gggccgtcgg
     1741 aggggaagtt cacgccgatg aacttcacct tgtagatgaa gcagccgtcc tgcagggagg
     1801 agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc tcccacttga
     1861 agccctcggg gaaggacagc ttcttgtagt cggggatgtc ggcggggtgc ttcacgtaca
     1921 ccttggagcc gtactggaac tggggggaca ggatgtccca ggcgaagggc agggggccgc
     1981 ccttggtcac cttcagcttc acggtgttgt ggccctcgta ggggcggccc tcgccctcgc
     2041 cctcgatctc gaactcgtgg ccgttcacgg tgccctccat gcgcaccttg aagcgcatga
     2101 actccttgat gacgttcttg gaggagcgca ccatctcgag gtcacttggt tgttcacgat
     2161 cttgtcgccg ccagtgtcaa cttgcaactg aaacaatatc caacatgaac gtcaatttat
     2221 actgccctaa tggcgaacac gataacaata tttcttttat tatgccctct aaaaccaacg
     2281 cggttatcgt ttatttattc aaattagata tagaacatcc gccgacatac aatgttaatg
     2341 caaaaacgcg tttggtgagc ggatacgaaa acagtcggcc gataaacatt aatctgaggt
     2401 cgataacacc gtccttgaac ggaacacgag gagcgtacgt gatcagctgc attcgcgcgc
     2461 cgcgccttta tcgagattta tttgcataca acaagtacac tgcgccgttg ggatttgtgg
     2521 taacgcgcac acatgcagag ctgcaagtgt ggcacatttt gtctgtgcgc aaaacctttg
     2581 aagccaaaag tacgaggtcc gttacgggca tgctagcgca cacggacaat ggacccgaca
     2641 aattctacgc caaggattta atgataatgt cgggcaacgt atccgttcat tttatcaata
     2701 acctacaaaa atgtcgcgcg catcacaaag acatcgacgc gcgtagaatt ctacccgtaa
     2761 agcgagttta gttatgagcc atgtgcaaaa catgacatca gcttttattt ttataacaaa
     2821 tgacatcatt tcttgattgt gttttacacg tagaattcta ctcgtaaagc gagttcagtt
     2881 ttgaaaaaca aatgacatca tctttttgat tgtgctttac aagtagaatt ctacccgtaa
     2941 atcaagttcg gttttgaaaa acaaatgagt catattgtat gatatcatat tgcaaaacaa
     3001 atgactcatc aatcgatcgt gcgttacacg tagaattcta ctcgtaaagc gagtttatga
     3061 gccgtgtgca aaacatgaca tcatctcgat ttgaaaaaca aatgacatca tccactgatc
     3121 gtgcgttaca agtagaattc tactcgtaaa gccagttcgg ttatgagccg tgtgcaaaac
     3181 atgacatcag cttatgactc atacttgatt gtgttttacg cggtttaaac gcgcccatac
     3241 tcggtggcct ccccaccacc aacttttttg cactgcaaaa aaacacgctt ttgcacgcgg
     3301 gcccatacat agtacaaact ctacgtttcg tagactattt tacataaata gtctacaccg
     3361 ttgtatacgc tccaaataca ctaccacaca ttgaaccttt ttgcagtgca aaaaagtacg
     3421 tgtcggcagt cacgtaggcc ggccttatcg ggtcgcgtcc tgtcacgtac gaatcacatt
     3481 atcggaccgg acgagtgttg tcttatcgtg acaggacgcc agcttcctgt gttgctaacc
     3541 gcagccggac gcaactcctt atcggaacag gacgcgcctc catatcagcc gcgcgttatc
     3601 tcatgcgcgt gaccggacac gaggcgcccg tcccgcttat cgcgcctata aatacagccc
     3661 gcaacgatct ggtaaacaca gttgaacagc atctgttaca gcgacacaac atgagccggt
     3721 ccaacaacgc caacgcgccc acgccatcca accgccgccg caacctgtct ctggtgatgg
     3781 tacccactat atagtataac cgatctgatc agattctgat ccagtgccat ctttttgttg
     3841 ttttttttct agtgtcatat tgtgtgaaat gtgaaatgga tgagaatgaa cgctggacga
     3901 ccagacagag aaagagaagc taggacaata ggactctcaa ctgcgcatta cgtggattcc
     3961 gtctccgacg atgcgccaaa cactatgcgt tagatgcatt gactaaccgc acactctttt
     4021 cacatagatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     4081 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     4141 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     4201 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     4261 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     4321 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     4381 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     4441 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     4501 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     4561 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     4621 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     4681 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     4741 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     4801 tcttgacgag ttcttctgaa gatcttcgac ctcgaaactt gtttattgca gcttataatg
     4861 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4921 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc atgtcgaggg
     4981 ggggcccgat ttaaataaac cccgggcgag ctcgaattaa ccattgtggg aaccgtgcga
     5041 tcaaacaaac gcgagatacc ggaagtactg aaaaacagtc gctccaggcc agtgggaaca
     5101 tcgatgtttt gttttgacgg accccttact ctcgtctcat ataaaccgaa gccagctaag
     5161 atggtatact tattatcatc ttgtgatgag gatgcttcta tcaacgaaag taccggtaaa
     5221 ccgcaaatgg ttatgtatta taatcaaact aaaggcggag tggacacgct agaccaaatg
     5281 tgttctgtga tgacctgcag taggaagacg aataggtggc ctatggcatt attgtacgga
     5341 atgataaaca ttgcctgcat aaattctttt attatataca gccataatgt cagtagcaag
     5401 ggagaaaagg ttcaaagtcg caaaaaattt atgagaaacc tttacatgag cctgacgtca
     5461 tcgtttatgc gtaagcgttt agaagctcct actttgaaga gatatttgcg cgataatatc
     5521 tctaatattt tgccaaatga agtgcctggt acatcagatg acagtactga agagccagta
     5581 atgaaaaaac gtacttactg tacttactgc ccctctaaaa taaggcgaaa ggcaaatgca
     5641 tcgtgcaaaa aatgcaaaaa agttatttgt cgagagcata atattgatat gtgccaaagt
     5701 tgtttctgac tgactaataa gtataatttg tttctattat gtataagtta agctaattac
     5761 ttattttata atacaacatg actgttttta aagtacaaaa taagtttatt tttgtaaaag
     5821 agagaatgtt taaaagtttt gttactttat agaagaaatt ttgagttttt gttttttttt
     5881 aataaataaa taaacataaa taaattgttt gttgaattta ttattagtat gtaagtgtaa
     5941 atataataaa acttaatatc tattcaaatt aataaataaa cctcgatata cagaccgata
     6001 aaacacatgc gtcaatttta cgcatgatta tctttaacgt acgtcacaat atgattatct
     6061 ttctagggtt aaataatagt ttctaatttt tttattattc agcctgctgt cgtgaatacc
     6121 gtatatctca acgctgtctg tgagattgtc gtattctagc ctttttagtt tttcgctcat
     6181 cgacttgata ttgtccgaca cattttcgtc gatttgcgtt ttgatcaaag acttgagcag
     6241 agacacgtta atcaactgtt caaattgatc catattaacg atatcaaccc gatgcgtata
     6301 tggtgcgtaa aatatatttt ttaaccctct tatactttgc actctgcgtt aatacgcgtt
     6361 cgtgtacaga cgtaatcatg ttttcttttt tggataaaac tcctactgag tttgacctca
     6421 tattagaccc tcacaagttg caaaacgtgg cattttttac caatgaagaa tttaaagtta
     6481 ttttaaaaaa tttcatcaca gatttaaaga agaaccaaaa attaaattat ttcaacagtt
     6541 taatcgacca gttaatcaac gtgtacacag acgcgtcggc aaaaaacacg cagcccgacg
     6601 tgttggctaa aattattaaa tcaacttgtg ttatagtcac ggatttgccg tccaacgtgt
     6661 tcctcaaaaa gttgaagacc aacaagttta cggacactat taattatttg attttgcccc
     6721 acttcatttt gtgggatcac aattttgtta tatttttaaa caaagcttgg cactggccgt
     6781 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6841 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6901 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
     6961 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     7021 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     7081 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     7141 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     7201 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     7261 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7321 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7381 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7441 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7501 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7561 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7621 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7681 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7741 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7801 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7861 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7921 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7981 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     8041 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     8101 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     8161 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     8221 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8281 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8341 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8401 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8461 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8521 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8581 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8641 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8701 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8761 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8821 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8881 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8941 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     9001 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     9061 atcccctgat tctgtggata
//
