LOCUS       sequence-257773-        5247 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector constitutively expressing Zea mays FNR and Z. mays SIR with 
            an insertion following BCD13 and preceeding the zmFNR gene deleted 
            'GGAGAGCATTAGGTCTCG'.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Wu B, Atkinson JT, Kahanda D, Bennett GN, Silberg JJ
  TITLE     Combinatorial design of chemical-dependent protein switches for 
            controlling intracellular electron transfer
  JOURNAL   bioRxiv May 23, 2019
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv May
            23, 2019"
COMMENT     Sequence Label: pBW004
FEATURES             Location/Qualifiers
     source          1..5247
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2..21
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     218..235
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        join(439..444,445..461,462..468)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     misc_feature    2309..2329
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     terminator      2384..2411
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        join(2431..2436,2437..2453,2454..2460)
                     /label=trc promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     terminator      3517..3588
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3604..3631
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             3792..4583
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     terminator      4595..4689
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      join(4803..5247,1..101)
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
ORIGIN
        1 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccaccactga tttgagcgtc
       61 agatttcgtg atgcttgtca ggggggcgga gcctatggaa aaacggcttt gccgcggccc
      121 tctcacttcc ctgttaagta tcttcctggc atcttccagg aaatctccgc cccgttcgta
      181 agccatttcc gctcgccgca gtcgaacgac cgagcgtagc gagtcagtga gcgaggaagc
      241 ggaatatatc ccctaggtgg agtctggcct caaatacaaa gtctgcaaag tctcgaaata
      301 ttaaaccaga gaataaataa ttaaacgcca aaatatttct agcaaaaacc ccagttatta
      361 aaccgcctaa gtcccccagg aaagggggat ataacagtat agattttgtc agccttcagc
      421 ttggctttac cgtcagagtt gacaattaat catccggctc gtataatgtg tggagggccc
      481 aagttcactt aaaaaggaga tcaacaatga aagcaatttt cgtactgaaa catcttaatc
      541 atgcaatgga ggctttctaa tggcgaagaa ggatgcttct gaagttaagc gaagcaaggt
      601 tgagataatc aaggaaaaga gcaacttcct ccggtaccct ttgaacgagg agttggtctc
      661 agaggcccca aatatcaacg agagtgctgt tcagctgatc aagtttcatg gaagctacca
      721 gcaaactgac cgagatgtcc gtgggcagaa gaattactcg tttatgctcc ggacaaagaa
      781 cccttgtggg aaagttccaa accaacttta tttagctatg gatacactag ccgatgagtt
      841 tggcatcgga acactccgcc tgacgaccag gcagacattc cagttgcacg gtgttcttaa
      901 gaagaacttg aagactgttc taagcactgt cataaagaat atgggctcaa cattgggtgc
      961 ttgtggcgat ctcaacagga atgtacttgc tcctgcagcg ccttatgtca aaaaagatat
     1021 cctttttgct caacaaacag cagagaacat tgcagcactt cttacaccac agtccggggc
     1081 ttattatgac ctgtgggtgg atggcgagaa gataatgtca gctgaagagc ctcctgaggt
     1141 gacaaaagcc cgcaatgaca actcgcatgg aacgaacttc cctgactctc cggaaccaat
     1201 ctatggcacc cagtatctac caaggaagtt caaggttgcg gttaccgcgg ctggtgataa
     1261 ctctgttgat attctgacca atgacatcgg tgttgttgtt gtttcagatg atgcaggaga
     1321 acctattggc tttaacatct atgttggtgg tggcatggga aggacacacc gagtggaaac
     1381 tacattccct cggctggctg atccattggg ttatgttcca aaagaagata tattatatgc
     1441 tataaaggcc attgtcgtca cacagaggga aaatggaaga agggatgacc gcaagtatag
     1501 taggatgaag tatatgattg accgttgggg aatagatagg tttcgggctg aagttgaaaa
     1561 atattacggg aagaagtttg aaagtttccg accattgcca gagtggcagt ttaacagcta
     1621 ccttggctgg caagaacagg gtgatgggaa attattctat ggagtgcatg ttgataatgg
     1681 tcgtgttggt gggcaagcaa agaaaactct acgagagata attgagaagt ataatttgga
     1741 tgttagtatt accccaaacc aaaatcttat tttatgtggg attgatcaag catggagaga
     1801 acccataact acagctcttg cacaagctgg cctgctggaa ccgaaggatg tcgacccctt
     1861 gaatttaact gccatggcat gccctgcctt gccactgtgc cctttggcac aaacagaagc
     1921 tgaacggggg atcttaccca ttcttaaacg aattagagca gtcttcaata aggttggtat
     1981 caaggattcg gagtctgtgg ttgtgaggat aactggatgc cctaatggat gcgctagacc
     2041 atatatggca gagcttggtt tcgttggtga tggcccaaaa agttaccaga tctggctggg
     2101 tggaacacca aaccagagta cgctagcaga atcatttatg gacaaggtga agcttgatga
     2161 catcgagaag gttttggagc ctctctttac ctattggaat ggcacacgcc aggaaggcga
     2221 atcttttgga agcttcacaa accgaacagg attcgacaaa ttgaaagagg tagtgaataa
     2281 gtgggcagag tcaccgtcag ccgcatgata ctagtagcgg ccgctgcagg catgcctcga
     2341 gatgcatggc gcctaaccta aactgacagg catcaaatta agcagaaggc catcctgacg
     2401 gatggccttt ttgcgtttcg aacaattgaa ttgacaatta atcatccggc tcgtataatg
     2461 tgtggagggc ccaagttcac ttaaaaagga gatcaacaat gaaagcaatt ttcgtactga
     2521 aacatcttaa tcatgcaatg gaggctttct aatgggcagt gttcaacagg cttctaggtc
     2581 aaaagtatcc gtcgcaccat tgcatttaga aagtgccaag gagcctccac taaatactta
     2641 taaacctaag gaaccattta cagctaccat tgtgagcgtt gagtctcttg taggtcctaa
     2701 agcaccagga gaaacttgtc acatagtcat cgatcatggc ggtaacgtgc cttactggga
     2761 aggacaatca tatggcgtta ttccacctgg tgagaatcca aagaaacctg gtgccccaca
     2821 gaacgtaagg ttgtactcca tagctagtac aagatatgga gacaatttcg atggcaggac
     2881 cggtagctta tgcgtcagaa gagcagttta ctatgaccct gaaactggaa aggaagatcc
     2941 atctaaaaac ggtgtgtgtt caaattttct atgcaactcc aagccaggtg acaaaatcca
     3001 acttacaggt cctagtggaa agattatgtt gttaccagag gaagatccta atgcaaccca
     3061 cataatgatc gctactggta caggcgttgc accattcagg ggttacttga gaagaatgtt
     3121 tatggaagat gtacctaact ataggttcgg aggtttagct tggctatttc ttggcgtcgc
     3181 caattctgac agcttgttat acgatgagga gttcacctca tatctaaaac aatacccaga
     3241 caattttaga tatgataagg cattgtccag ggagcagaaa aacagaagtg gaggtaaaat
     3301 gtacgtgcaa gataagattg aagaatattc tgacgagatt ttcaaattac ttgatggcgg
     3361 tgctcatata tacttttgtg gattgaaggg tatgatgcct ggcatccaag acactttaaa
     3421 aaaggttgcc gaaagaaggg gtgaatcatg ggatcagaaa ctagcacaac ttaagaaaaa
     3481 taagcagtgg cacgtagagg tctattaacc aggcatcaaa taaaacgaaa ggctcagtcg
     3541 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     3601 actggctcac cttcgggtgg gcctttctgc gtttataggt accagcaagc gaaccggaat
     3661 tgccagctgg ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt
     3721 tcttgccgcc aaggatctga tggcgcaggg gatcaagatc tgatcaagag acaggatgag
     3781 gatcgtttcg tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag
     3841 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg
     3901 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa
     3961 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc
     4021 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca
     4081 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca
     4141 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa
     4201 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg
     4261 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc
     4321 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg
     4381 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc
     4441 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag
     4501 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga
     4561 tcaccaaggt agtcggcaaa taagagctcg cttggactcc tgttgataga tccagtaatg
     4621 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
     4681 ggtgagaatc caagcactag taacaactta tatcgtatgg ggctgacttc aggtgctaca
     4741 tttgaagaga taaattgcac tgaaatctag taatatttta tctgattaat aagatgatct
     4801 tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg
     4861 cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt
     4921 ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc
     4981 aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct
     5041 ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg
     5101 gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg
     5161 tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa
     5221 caggagagcg cacgagggag ccgccag
//
