LOCUS       sequence-25781-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Elde NC, Child SJ, Geballe AP, Malik HS
  TITLE     Protein kinase R reveals an evolutionary model for defeating viral 
            mimicry.
  JOURNAL   Nature. 2009 Jan 22;457(7228):485-9. doi: 10.1038/nature07529. Epub 
            2008 Nov 30.
  PUBMED    19043403
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07529"; journalName: "Nature"; date: "2009-01-22- 
            22"; volume: "457"; issue: "7228"; pages: "485-9"
COMMENT     Sequence Label: pSB305-K3L-vac-HA
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        25..689
                     /gene="GAL1 and GAL10 genes of S. cerevisiae"
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     protein_bind    241..358
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     653..676
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             703..729
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 aaaagctggt accttatatt gaattttcaa aaattcttnc nnnnnttttg gatggacgca
       61 aagaagttta ataatcatat tacatggcat taccaccata tacatatcca tatacatatc
      121 catatctaat cttacttata tgttgtggaa atgtaaagag ccccattatc ttagcctaaa
      181 aaaaccttct ctttggaact ttcagtaata cgcttaactg ctcattgcta tattgaagta
      241 cggattagaa gccgccgagc gggtgacagc cctccgaagg aagactctcc tccgtgcgtc
      301 ctcgtcttca ccggtcgcgt tcctgaaacg cagatgtgcc tcgcgccgca ctgctccgaa
      361 caataaagat tctacaatac tagcttttat ggttatgaag aggaaaaatt ggcagtaacc
      421 tggccccaca aaccttcaaa tgaacgaatc aaattaacaa ccataggatg ataatgcgat
      481 tagtttttta gccttatttc tggggtaatt aatcagcgaa gcgatgattt ttgatctatt
      541 aacagatata taaatgcaaa aactgcataa ccactttaac taatactttc aacattttcg
      601 gtttgtatta cttcttattc aaatgtaata aaagtatcaa caaaaaattg ttaatatacc
      661 tctatacttt aacgtcaagg agaaaaaact atactcgaga tgtacccata cgacgttcca
      721 gactacgcta tgcttgcatt ttgttattcg ttgcccnatg cgggcnatgt aataaa
//
