LOCUS       sequence-25783-e         646 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Elde NC, Child SJ, Geballe AP, Malik HS
  TITLE     Protein kinase R reveals an evolutionary model for defeating viral 
            mimicry.
  JOURNAL   Nature. 2009 Jan 22;457(7228):485-9. doi: 10.1038/nature07529. Epub 
            2008 Nov 30.
  PUBMED    19043403
REFERENCE   2  (bases 1 to 646)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature07529"; journalName: "Nature"; date: "2009-01-22- 
            22"; volume: "457"; issue: "7228"; pages: "485-9"
COMMENT     Sequence Label: pSB305-K3L-var
FEATURES             Location/Qualifiers
     source          1..646
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(292..311)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(293..311)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(318..335)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(318..334)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(327..349)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(562..581)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ttggttttct gttactcttt gccaaacgtt ggtgacgttt tgaagggtaa ggttacgnna
       61 acggttacgt tttgtacgtc gacttgttcg actacccaca ctctgaagct atcttggttg
      121 aatctaccca aatgcatatg aacagatact tcaagtacag agacaagttg gttggtaaga
      181 ccgttaaggt taaggttatc agaatcgact acaccaaggg ttacatcgac gttaactaca
      241 agagaatgtg tagacactga gcggccgcca ccgcggtgga gctccaattc gccctatagt
      301 gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
      361 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
      421 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggacgcgc
      481 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
      541 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
      601 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggtt
//
