LOCUS       sequence-25786-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Springer BA, Sligar SG
  TITLE     High-level expression of sperm whale myoglobin in Escherichia coli.
  JOURNAL   Proc Natl Acad Sci U S A. 1987 Dec . 84(24):8961-5.
  PUBMED    3321062
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1987 Dec . 84(24):8961-5."
COMMENT     Sequence Label: pMB413a
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(543..560)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(543..559)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(552..574)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 cacnnacana attcgccagc tctgcagata actaactaaa ggagaacaac aacaatggtt
       61 ctgtctgaag gtgaatggca gctggttctg catgtttggg ctaaagttga agctgacgtc
      121 gctggtcatg gtcaggacat cttgattcga ctgttcaaat ctcatccgga aactctggaa
      181 aaattcgatc gtttcaaaca tctgaaaact gaagctgaaa tgaaagcttc tgaagatctg
      241 aaaaaacatg gtgttaccgt gttaactgcc ctaggtgcta tccttaagaa aaaagggcat
      301 catgaagctg agctcaaacc gcttgcgcaa tcgcatgcta ctaaacataa gatcccgatc
      361 aaatacctgg aattcatctc tgaagcgatc atccatgttc tgcattctag acatccaggt
      421 aacttcggtg ctgacgctca gggtgctatg aacaaagctc tcgagctgtt ccgtaaagat
      481 atcgctgcta agtacaaaga actgggttac cagggttaat gaggtaccgg ggatcctaat
      541 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
      601 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
      661 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
      721 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
      781 gangccncaa nttaancagc ccgaccccgc aacaccgctg ancgccctga cggnttgtct
      841 gncccng
//
