LOCUS       sequence-25787-e         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Beck von Bodman S, Schuler MA, Jollie DR, Sligar SG
  TITLE     Synthesis, bacterial expression, and mutagenesis of the gene coding 
            for mammalian cytochrome b5.
  JOURNAL   Proc Natl Acad Sci U S A. 1986 Dec . 83(24):9443-7.
  PUBMED    3540940
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 1986 Dec . 83(24):9443-7."
COMMENT     Sequence Label: pVS45
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(359..376)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(359..375)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(368..390)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(584..603)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     703..725
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 aatngnanga ttcgccagct tgggctgcag ataaaggaga acaacaacaa tggctgaaca
       61 aagcgacaaa gacgtcaaat actacactct ggaagaaatc caaaaacaca aagactcgaa
      121 gtcgacgtgg gtgatcctgc accataaagt atacgaccta actaaattcc tcgaagagca
      181 ccccgggggc gaagaagtcc tgagagaaca ggccggcggt gacgcgactg aaaacttcga
      241 agacgttggc catagtaccg acgctcgaga actgtcgaaa acgtacatca tcggtgagct
      301 gcacccggac gatcgttcta aaatcgcgaa accgtccgaa actctgtaat gagaattcac
      361 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
      421 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
      481 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
      541 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
      601 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
      661 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
      721 agaggttttc acgttcttcn cctgancgcn gnnacnangg gcctcgtgat accctatttt
      781 tataggtaat gtcatgataa tatggtttct nanacgtcag nggcctttcg g
//
