LOCUS       sequence-25798-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Aasen T, Raya A, Barrero MJ, Garreta E, Consiglio A, Gonzalez F, 
            Vassena R, Bilic J, Pekarik V, Tiscornia G, Edel M, Boue S, Belmonte
            JC
  TITLE     Efficient and rapid generation of induced pluripotent stem cells 
            from human keratinocytes.
  JOURNAL   Nat Biotechnol. 2008 Nov . 26(11):1276-84.
  PUBMED    18931654
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2008 Nov . 26(11):1276-84."
COMMENT     Sequence Label: pMSCV-Flag-hOct4
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             28..51
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctcctgaata gatctgccgc cgccatggac tacaaggacg acgatgacaa gctcgaggtt
       61 aacgaattca tggcgggaca cctggcttcg gatttcgcct tctcgccccc tccaggtggt
      121 ggaggtgatg ggccaggggg gccggagccg ggctgggttg atcctcggac ctggctaagc
      181 ttccaaggcc ctcctggagg gccaggaatc gggccggggg ttgggccagg ctctgaggtg
      241 tgggggattc ccccatgccc cccgccgtat gagttctgtg gggggatggc gtactgtggg
      301 ccccaggttg gagtggggct agtgccccaa ggcggcttgg agacctctca gcctgagggc
      361 gaagcaggag tcggggtgga gagcaactcc gatggggcct ccccggagcc ctgcaccgtc
      421 acccctggtg ccgtgaagct ggagaaggag aagctggagc aaaacccgga ggagtcccag
      481 gacatcaaag ctctgcagaa agaactcgag caatttgcca agctcctgaa gcagaagagg
      541 atcaccctgg gatatacaca ggccgatgtg gggctcaccc tgggggttct atttgggaag
      601 gtattcagcc aaacgaccat ctgccgcttt gaggctctgc agcttanctt caagaacatg
      661 tgtaagctgc ggcccttgct ncagaagtgg gnggaggaag ctgacaacaa tgaaaacctt
      721 cangagatat gcaaagcana aaccctcgtg caggcccgaa agagaancga accantatcn
      781 agaacc
//
