LOCUS       sequence-25800-e         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Aasen T, Raya A, Barrero MJ, Garreta E, Consiglio A, Gonzalez F, 
            Vassena R, Bilic J, Pekarik V, Tiscornia G, Edel M, Boue S, Belmonte
            JC
  TITLE     Efficient and rapid generation of induced pluripotent stem cells 
            from human keratinocytes.
  JOURNAL   Nat Biotechnol. 2008 Nov . 26(11):1276-84.
  PUBMED    18931654
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2008 Nov . 26(11):1276-84."
COMMENT     Sequence Label: pMSCV-Flag-hKlf4
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      11..20
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             20..43
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgatctgcc gccgccatgg actacaagga cgacgatgac aagctcgagg ttaacgaatt
       61 catggctgtc agcgacgcgc tgctcccatc tttctccacg ttcgcgtctg gcccggcggg
      121 aagggagaag acactgcgtc aagcaggtgc cccgaataac cgctggcggg aggagctctc
      181 ccacatgaag cgacttcccc cagtgcttcc cggccgcccc tatgacctgg cggcggcgac
      241 cgtggccaca gacctggaga gcggcggagc cggtgcggct tgcggcggta gcaacctggc
      301 gcccctacct cggagagaga ccgaggagtt caacgatctc ctggacctgg actttattct
      361 ctccaattcg ctgacccatc ctccggagtc agtggccgcc accgtgtcct cgtcagcgtc
      421 agcctcctct tcgtcgtcgc cgtcgagcag cggccctgcc agcgcgccct ccacctgcag
      481 cttcacctat ccgatccggg ccgggaacga cccgggcgtg gcgccgggcg gcacgggcgg
      541 aggcctcctc tatggcaggg agtccgctcc ccctccgacg gctcccttca acctggcgga
      601 catcaacgac gtgagcccct cgggcggctt cgtggccgag ctcctgcggc cagaattgga
      661 cccggtgtac attccgccgc agcagccgca gccg
//
