LOCUS       sequence-25803-e         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  TITLE     Gitler lab plasmids
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PCRII-zSynA
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(488..507)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(489..507)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(523..545)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 atagctggta cgagctcgga tccactagta acggccgcca gtgtgctgga attcgccctt
       61 tattaatcca taaacctgaa gctgaaacgg tccccccctc tccacctcta cgtttctgct
      121 ccaaagcctc acccgaccag acttccagat cttactgata tttttctgtg actgtatgga
      181 aaaccagagt tgaaaaagac tttccctgag ccctgtagcc tttagatgtg tgacgcttcc
      241 ctcctttaca tctgtggtgt cgtcatgctg ttctctagtg ttactgtcaa tcctctgcct
      301 gttcacaaca cacaaaacac acacacactc caacacgtac ttcctctcac acctcccccc
      361 cccccaaaaa atgaacagga accgtaaagc ccagaggctg atattagaga ttggaggtaa
      421 gggcgaattc tgcagatatc catcacactg gcggccgctc gagcatgcat ctagagggnc
      481 caattcgccc tatagtgagt cgtattacaa ttcactggnc gtcgttttac aacgtcgtga
      541 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
      601 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
      661 tggcgaatgg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      721 agcgtgaccg ctacacttgn cagcgnccta gcgncngntc tttcgctttc tctttntttn
      781 tcgccngttg tnngnttncc ggaannttna atggg
//
