LOCUS       sequence-25804-e         768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Czlapinski JL, Schelle MW, Miller LW, Laughlin ST, Kohler JJ, 
            Cornish VW, Bertozzi CR
  TITLE     Conditional glycosylation in eukaryotic cells using a biocompatible 
            chemical inducer of dimerization.
  JOURNAL   J Am Chem Soc. 2008 Oct 8. 130(40):13186-7.
  PUBMED    18788807
REFERENCE   2  (bases 1 to 768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2008 Oct 8. 130(40):13186-7."
COMMENT     Sequence Label: FKBP-myc
FEATURES             Location/Qualifiers
     source          1..768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(23..52)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(53..373)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     primer_bind     complement(488..507)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(489..507)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(552..756)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(581..601)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gnnnnnnacg ggctctagtc tacagatcct cttctgagat gagtttttgt tcttccagtt
       61 ttagaagctc cacatcgaag acgagagtgg catgtggtgg gatgatgcct gggtgcccag
      121 ttgcaccata ggcataatct ggagatatag tcagtttggc tctctgaccc acactcatct
      181 gggcaacccc ttcttcccag cctcggatca cctcctgctt gcctagcata aacttaaagg
      241 gcttgtttct gtcccgggag gaatcaaatt tctttccatc ttcaagcatc ccggtgtagt
      301 gcaccacgca ggtctggccg cgcttgggga aggtgcgccc gtctcctggg gagatggttt
      361 ccacctgcac tcctctagac tcgagcggcc gccactgtgc tggatatctg cagaattcca
      421 ccacactgga ctagtggatc cgagctcggt accaagctta agtttaaacg ctagccagct
      481 tgggtctccc tatagtgagt cgtattaatt tcgataagcc agtaagcagt gggttctcta
      541 gttagccaga gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc
      601 gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac
      661 aaactcccat tgacgtcaat gggggtggag acttggaaat ccccgtgagt caaaccgcta
      721 tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagc
//
