LOCUS       sequence-25805-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Czlapinski JL, Schelle MW, Miller LW, Laughlin ST, Kohler JJ, 
            Cornish VW, Bertozzi CR
  TITLE     Conditional glycosylation in eukaryotic cells using a biocompatible 
            chemical inducer of dimerization.
  JOURNAL   J Am Chem Soc. 2008 Oct 8. 130(40):13186-7.
  PUBMED    18788807
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2008 Oct 8. 130(40):13186-7."
COMMENT     Sequence Label: 2xFKBP-myc
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(16..45)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(46..366)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             complement(373..693)
                     /codon_start=1
                     /product="human FK506-binding protein FKBP12"
                     /label=FKBP
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKFDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     gap             781..790
                     /estimated_length=10
ORIGIN
        1 cggnncncta gtctacagat cctcttctga gatgagtttt tgttcttcca gttttagaag
       61 ctccacatcg aagacgagag tggcatgtgg tgggatgatg cctgggtgcc cagttgcacc
      121 ataggcataa tctggagata tagtcagttt ggctctctga cccacactca tctgggcaac
      181 cccttcttcc cagcctcgga tcacctcctg cttgcctagc ataaacttaa agggcttgtt
      241 tctgtcccgg gaggaatcaa atttctttcc atcttcaagc atcccggtgt agtgcaccac
      301 gcaggtctgg ccgcgcttgg ggaaggtgcg cccgtctcct ggggagatgg tttccacctg
      361 cactcctcta gtttccagtt ttagaagctc cacatcgaag acgagagtgg catgtggtgg
      421 gatgatgcct gggtgcccag tggcaccata ggcataatct ggagatatag tcagtttggc
      481 tctctgaccc acactcatct gggcaacccc ttcttcccag cctcggatca cctcctgctt
      541 gcctagcata aacttaaagg gcttgtttct gtcccgggag gaatcaaatt tctttccatc
      601 ttcaagcatc ccggtgtagt gcaccacgca ggtctggccg cgctttggga aggtgcgccc
      661 gtctcctggg gagatggttt ccacctgcac tcctctagac tcgagcggcc gccactgtgc
      721 tggatatcnn nncaantccc cccncncnnn gnnnnnnnng gnntcccnan cctcgngnnc
      781 nnnnnnnnnn atgtttaaac cnntancccn gcct
//
