LOCUS       sequence-258060-        8571 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression of CENP-U CENP-Q CENP-OP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8571)
  AUTHORS   McKinley KL, Sekulic N, Guo LY, Tsinman T, Black BE, Cheeseman IM
  TITLE     The CENP-L-N Complex Forms a Critical Node in an Integrated Meshwork
            of Interactions at the Centromere-Kinetochore Interface.
  JOURNAL   Mol Cell. 2015 Dec 17;60(6):886-98. doi: 
            10.1016/j.molcel.2015.10.027. Epub 2015 Nov 19.
  PUBMED    26698661
REFERENCE   2  (bases 1 to 8571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2015.10.027"; journalName: "Mol Cell"; date: 
            "2015-12-17- 17"; volume: "60"; issue: "6"; pages: "886-98"
COMMENT     Sequence Label: pKM499
FEATURES             Location/Qualifiers
     source          1..8571
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      190..778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     679..698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     mobile_element  913..1136
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1203..1736)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1925..1930,1931..1947,1948..1953))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2024..2057)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        2134..2225
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    3722..3856
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4021..4112
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    5146..5280
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5466..5557
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    6687..6821
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        6993..7084
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    8178..8312
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 accggttgac ttgggtcaac tgtcagacca agtttactca tatatacttt agattgattt
       61 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
      121 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
      181 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
      241 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
      301 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
      361 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
      421 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
      481 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
      541 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
      601 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
      661 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
      721 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
      781 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
      841 ctttcctgcg ttatcccctg attgacttgg gtcgctcttc ctgtggatgc gcagatgccc
      901 tgcgtaagcg ggtgtgggcg gacaataaag tcttaaactg aacaaaatag atctaaacta
      961 tgacaataaa gtcttaaact agacagaata gttgtaaact gaaatcagtc cagttatgct
     1021 gtgaaaaagc atactggact tttgttatgg ctaaagcaaa ctcttcattt tctgaagtgc
     1081 aaattgcccg tcgtattaaa gaggggcgtg gccaagggca tgtaaagact atattcgcgg
     1141 cgttgtgaca atttaccgaa caactccgcg gccgggaagc cgatctcggc ttgaacgaat
     1201 tgttaggtgg cggtacttgg gtcgatatca aagtgcatca cttcttcccg tatgcccaac
     1261 tttgtataga gagccactgc gggatcgtca ccgtaatctg cttgcacgta gatcacataa
     1321 gcaccaagcg cgttggcctc atgcttgagg agattgatga gcgcggtggc aatgccctgc
     1381 ctccggtgct cgccggagac tgcgagatca tagatataga tctcactacg cggctgctca
     1441 aacttgggca gaacgtaagc cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca
     1501 agcgcgatga atgtcttact acggagcaag ttcccgaggt aatcggagtc cggctgatgt
     1561 tgggagtagg tggctacgtc tccgaactca cgaccgaaaa gatcaagagc agcccgcatg
     1621 gatttgactt ggtcagggcc gagcctacat gtgcgaatga tgcccatact tgagccacct
     1681 aactttgttt tagggcgact gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt
     1741 gctgctccat aacatcaaac atcgacccac ggcgtaacgc gcttgctgct tggatgcccg
     1801 aggcatagac tgtacaaaaa aacagtcata acaagccatg aaaaccgcca ctgcgccgtt
     1861 accaccgctg cgttcggtca aggttctgga ccagttgcgt gagcgcatac gctacttgca
     1921 ttacagttta cgaaccgaac aggcttatgt caactgggtt cgtgccttca tccgtttcca
     1981 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gccataactt cgtatagcat
     2041 acattatacg aagttatctg taactataac ggtcctaagg tagcgagttt aaacactagt
     2101 atcgattcgc gacctactcc ggaatattaa tagatcatgg agataattaa aatgataacc
     2161 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
     2221 taaatattcc ggattattca taccgtccca ccatcgggcg cggatccatg gccccgcgcg
     2281 gtcgtcgccg tcctcgtccg caccgtagcg aaggtgcccg tcgtagcaag aacaccctgg
     2341 agcgcaccca cagcatgaag gacaaggccg gtcagaagtg caaacctatc gacgtgttcg
     2401 acttcccgga caacagcgac gtgagtagta tcggccgcct gggtgagaat gagaaggacg
     2461 aggagaccta cgagacattc gatccgcctc tgcatagcac cgccatttac gcagacgaag
     2521 aggagttcag caagcactgc ggcctgagct taagcagcac cccgcctggc aaggaagcaa
     2581 agcgtagcag tgacaccagt ggcaatgagg ccagtgaaat cgagagcgtg aagatcagcg
     2641 caaagaagcc tggccgtaag ctgcgcccga tcagcgacga tagcgagagc attgaggaga
     2701 gtgatacccg ccgcaaggtg aaaagcgcag agaagatcag cacccagcgc cacgaagtta
     2761 ttcgcaccac agcaagcagc gaactgagcg agaaaccggc cgaaagcgtg acaagtaaaa
     2821 agacaggccc gctgagcgca caacctagcg tggaaaagga gaacctggcc atcgaaagcc
     2881 agagtaagac ccaaaaaaaa ggtaagatta gccatgacaa gcgcaagaag agccgtagca
     2941 aggccatcgg cagcgacacc agtgacatcg tgcacatttg gtgcccggag ggtatgaaaa
     3001 ccagcgacat taaagagctg aacatcgtgc tgccggagtt tgagaagacc cacctggaac
     3061 accagcagcg catcgagagt aaggtgtgca aggccgccat cgccaccttt tacgttaatg
     3121 ttaaagagca gtttattaaa atgctgaagg agagccagat gctgaccaac ctgaagcgca
     3181 agaacgccaa gatgatcagc gacatcgaga agaagcgcca gcgcatgatc gaggtgcagg
     3241 acgaattact gcgcctggag ccgcagctga agcaactgca gacaaagtac gacgagctga
     3301 aggagcgcaa gagcagcctg cgcaacgcag cctactttct gagcaacctg aaacagttat
     3361 atcaagatta cagcgatgtg caggcacagg agccgaatgt gaaagagaca tacgacagca
     3421 gtagtctgcc tgccttactg ttcaaggccc gtaccctgtt aggtgcagag agccatctgc
     3481 gcaacatcaa ccaccagctg gaaaagttac tggaccaggg cgaattcaaa ggcctacgtc
     3541 gacgagctca cttgtcgcgg ccgctttcga atctagagcc tgcagtctcg acaagcttgt
     3601 cgagaagtac tagaggatca taatcagcca taccacattt gtagaggttt tacttgcttt
     3661 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     3721 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     3781 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     3841 ttatcatgtc tggatctgat cactgcttga gcctagaaga tccggctgct aacaaagccc
     3901 gaaaggaagc tgagttggct gctgccaccg ctgagcaata actatcataa cccctagggt
     3961 atacccatag cgagtttaaa cactagtatc gattcgcgac ctactccgga atattaatag
     4021 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
     4081 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
     4141 tcgggcgcgg atccatgagc ggcaaggcca acgccagcaa gaagaatgcc cagcagctga
     4201 agcgcaatcc gaagcgtaag aaggacaacg aagaggtggt gctgagcgag aacaaggtgc
     4261 gcaacaccgt gaagaagaat aaaaaccacc tgaaagacct gagcagcgag ggccagacca
     4321 agcatacaaa cctgaagcac ggcaagaccg ccgccagcaa gcgtaaaacc tggcagcctc
     4381 tgagcaagag cacccgtgac cacctgcaga ccatgatgga gagcgtgatc atgaccatcc
     4441 tgagtaacag catcaaggag aaagaggaga tccagtatca tctgaacttc ctgaagaagc
     4501 gtctgctgca acagtgcgag accctgaagg tgccgccgaa gaagatggag gatctgacca
     4561 acgtgagcag cctgctgaac atggagcgcg cacgcgacaa ggcaaacgaa gaggggctgg
     4621 ccctgctgca agaggagatc gacaagatgg tggagaccac agagctgatg accggcaaca
     4681 tccaaagcct gaagaacaag atccagatcc tggccagcga ggttgaagaa gaagaggagc
     4741 gcgtgaaaca gatgcaccag atcaacagca gcggcgtgct gagcttaccg gaactgagcc
     4801 agaaaaccct gaaggcaccg accctgcaga aggaaatcct ggccctgatt ccgaaccaga
     4861 acgccctgct gaaggacctg gacatcctgc acaacagcag ccagatgaag agcatgagca
     4921 cattcatcga ggaagcctac aagaagctgg acgccagtta agtcgacgag ctcacttgtc
     4981 gcggccgctt tcgaatctag agcctgcagt ctcgacaagc ttgtcgagaa gtactagagg
     5041 atcataatca gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac
     5101 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     5161 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     5221 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     5281 tgatcactgc ttgagcctag aagatccggc tgctaacaaa gcccgaaagg aagctgagtt
     5341 ggctgctgcc accgctgagc aataactatc ataaccccta gggtatactc taattggaac
     5401 cagataagtg aatagcgagt ttaaacacta gtatcgattc gcgacctact ccggaatatt
     5461 aatagatcat ggagataatt aaaatgataa ccatctcgca aataaataag tattttactg
     5521 ttttcgtaac agttttgtaa taaaaaaacc tataaatatt ccggattatt cataccgtcc
     5581 caccatcggg cgcggatcca tggaacaggc aaatccgctg cgcccagacg gtgagtcaaa
     5641 aggtggtgtg ctggctcatc tggaacgcct ggaaacacag gttagccgta gtcgtaaaca
     5701 gagcgaagaa ctgcagtcag tacaagctca ggagggtgcc ctgggtacaa aaatccacaa
     5761 actgcgtcgc ctgcgtgatg aactgcgtgc tgttgtccgt catcgtcgtg ccagtgttaa
     5821 agcgtgtatc gccaacgttg aaccgaatca gacagtcgag atcaatgaac aagaagcgct
     5881 ggaggaaaaa ctggagaacg tcaaagccat tctgcaagcc tatcacttta ccggtctgtc
     5941 agggaaactg acctctcgtg gggtgtgtgt gtgtattagt accgccttcg agggaaatct
     6001 gctggactcc tatttcgttg acctggtgat ccaaaaacca ctgcgcatcc accatcattc
     6061 agtgccggtc tttattccgc tggaagagat tgccgccaaa tatctgcaaa ccaacatcca
     6121 acattttctg ttcagcctgt gtgagtatct gaatgcctat agcgggcgta aatatcaggc
     6181 cgatcgcctg caaagcgatt ttgctgctct gctgacgggt cctctgcagc gtaaccctct
     6241 gtgtaatctg ctgtccttca cctataaact ggaccctggt ggtcagagtt ttccgttttg
     6301 tgctcgtctg ctgtataaag acctgaccgc cacactgcct acagatgtta ccgtgacatg
     6361 ccagggagtt gaagtcctgt ctaccagctg ggaagaacaa cgtgcctcac atgaaaccct
     6421 gttctgtacc aaaccgctgc atcaggtgtt tgccagcttt actcgtaaag gcgagaaact
     6481 ggacatgagt ctggtgtcct aagtcgacga gctcacttgt cgcggccgct ttcgaatcta
     6541 gagcctgcag tctcgacaag cttgtcgaga agtactagag gatcataatc agccatacca
     6601 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
     6661 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
     6721 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6781 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg cttgagccta
     6841 gaagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag
     6901 caataactat cataacccct agggtatacc catctaaggt agcgagttta aacactagta
     6961 tcgattcgcg acctactccg gaatattaat agatcatgga gataattaaa atgataacca
     7021 tctcgcaaat aaataagtat tttactgttt tcgtaacagt tttgtaataa aaaaacctat
     7081 aaatattccg gattattcat accgtcccac catcgggcgc ggatccatgg atgccgaact
     7141 ggctgaagtc cgtgctctgc aagctgagat cgctgctctg cgtcgtgcct gtgaagatcc
     7201 tccggctcct tgggaagaaa aatcccgtgt ccagaaatca tttcaggcca ttcatcagtt
     7261 taacctggag gggtggaaaa gctctaaaga cctgaaaaac cagctgggtc atctggaatc
     7321 agagctgtcg tttctgtcta ccctgaccgg cattaacatt cgtaaccact caaaacaaac
     7381 cgaggacctg acctctacag agatgacgga gaaatccatc cgtaaagtgc tgcagcgcca
     7441 tcgtctgtct ggaaattgcc acatggtgac ctttcaactg gagttccaaa tcctggagat
     7501 tcagaacaaa gaacgcctgt ctagtgctgt tactgatctg aacatcatca tggagccaac
     7561 cgaatgctcc gaactgagcg aatttgttag ccgtgccgaa gagcgtaaag acctgttcat
     7621 gttttttcgt tcactgcatt tttttgtgga atggttcgag tatcgtaaac gcaccttcaa
     7681 acatctgaaa gagaaatatc ctgacgccgt gtatctgtcc gaaggccctt caagttgtag
     7741 catgggtatc cgctcggcct ctcgtccggg gttcgaactg gttattgtgt ggcgtattca
     7801 aatcgatgag gacggcaaag tgttcccgaa actggatctg ctgaccaaag ttcctcaacg
     7861 tgccctggag ctggataaaa atcgtgccat cgaaaccgct cctctgtctt tccgtaccct
     7921 ggttggtctg ctgggtattg aagcagccct ggaaagcctg attaaaagtc tgtgtgccga
     7981 ggagaacaac tgagtcgacg agctcacttg tcgcggccgc tttcgaatct agagcctgca
     8041 gtctcgacaa gcttgtcgag aagtactaga ggatcataat cagccatacc acatttgtag
     8101 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     8161 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     8221 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8281 aactcatcaa tgtatcttat catgtctgga tctgatcact gcttgagcct agaagatccg
     8341 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
     8401 tcataacccc tagggtatac atctagttcc aaactatttt gtcattttta attttcgtat
     8461 tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt
     8521 aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac a
//
