LOCUS       sequence-25807-e         732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Czlapinski JL, Schelle MW, Miller LW, Laughlin ST, Kohler JJ, 
            Cornish VW, Bertozzi CR
  TITLE     Conditional glycosylation in eukaryotic cells using a biocompatible 
            chemical inducer of dimerization.
  JOURNAL   J Am Chem Soc. 2008 Oct 8. 130(40):13186-7.
  PUBMED    18788807
REFERENCE   2  (bases 1 to 732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2008 Oct 8. 130(40):13186-7."
COMMENT     Sequence Label: DHFR-myc
FEATURES             Location/Qualifiers
     source          1..732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..47)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(48..521)
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     primer_bind     complement(636..655)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(637..655)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgggccctc tagtctacag atcctcttct gagatgagtt tttgttcccg ccgctccaga
       61 atctcaaagc aatagctgtg agagttctgc gcatcagcat cgtggaattc gctgaatacc
      121 gattcccagt catccggctc gtaatccggg aaatgggtgt cgccttccac ttctgcgtcg
      181 atatgcgtca gatacagttt ttgcgctttt ggcaagaact gttcataaac gcgaccgccg
      241 ccaatcacca tgatttctgg tacgtcacca cacgccgcga tggcttcatc caccgacttc
      301 acccacgtta cgcgatcgtc cgtacccggt tgactgctga ggataatatt tttgcgtcct
      361 ggcaacggac gaccgattga ttcccaggta tggcggccca taatcacggg tttatttaag
      421 gtgttgcgtt taaaccaggc gagatcggca ggcaggttcc acggcatggc gttttccatg
      481 ccgataacgc gatctaccgc taacgccgca atcagactga ttctagactc gagcggccgc
      541 cactgtgctg gatatctgca gaattccacc acactggact agtggatccg agctcggtac
      601 caagcttaag tttaaacgct agccagcttg ggtctcccta tagtgagtcg tattaatttc
      661 gataagccag taagcagtgg gttctctagt tagccagaga gctctgctta tatagacctc
      721 ccaccgtaca cg
//
