LOCUS       sequence-25808-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Czlapinski JL, Schelle MW, Miller LW, Laughlin ST, Kohler JJ, 
            Cornish VW, Bertozzi CR
  TITLE     Conditional glycosylation in eukaryotic cells using a biocompatible 
            chemical inducer of dimerization.
  JOURNAL   J Am Chem Soc. 2008 Oct 8. 130(40):13186-7.
  PUBMED    18788807
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2008 Oct 8. 130(40):13186-7."
COMMENT     Sequence Label: 2xDHFR-myc
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..42)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(43..516)
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
ORIGIN
        1 gnctctagtc tacagatcct cttctgagat gagtttttgt tcccgccgct ccagaatctc
       61 aaagcaatag ctgtgagagt tctgcgcatc agcatcgtgg aattcgctga ataccgattc
      121 ccagtcatcc ggctcgtaat ccgggaaatg ggtgtcgcct tccacttctg cgtcgatatg
      181 cgtcagatac agtttttgcg cttttggcaa gaactgttca taaacgcgac cgccgccaat
      241 caccatgatt tctggtacgt caccacacgc cgcgatggct tcatccaccg acttcaccca
      301 cgttacgcga tcgtccgtac ccggttgact gctgaggata atatttttgc gtcctggcaa
      361 cggacgaccg attgattccc aggtatggcg gcccataatc acgggtttat ttaaggtgtt
      421 gcgtttaaac caggcgagat cggcaggcag gttccacggc atggcgtttt ccatgccgat
      481 aacgcgatct accgctaacg ccgcaatcag actgattcta gtccgccgct ccagaatctc
      541 aaagcaatag ctgtgagagt tctgcgcatc agcatcgtgg aattcgctga ataccgattc
      601 ccagtcatcc ggctcgtaat ccgggaaatg ggtgtcgcct tccacttctg cgtcgatatg
      661 cgtcagatac agtttttgcg cttttggcaa gaactgttca tnaaacgcga ncgccgncaa
      721 ncacncatga ttttctgggt angtcnccca ccaccccnnc gatgggcttn nnnnnccgaa
      781 ntctccaccc ancgtntacg cgatcgtccc gtaacccggg ttga
//
