LOCUS       sequence-25809-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Czlapinski JL, Schelle MW, Miller LW, Laughlin ST, Kohler JJ, 
            Cornish VW, Bertozzi CR
  TITLE     Conditional glycosylation in eukaryotic cells using a biocompatible 
            chemical inducer of dimerization.
  JOURNAL   J Am Chem Soc. 2008 Oct 8. 130(40):13186-7.
  PUBMED    18788807
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Am Chem 
            Soc. 2008 Oct 8. 130(40):13186-7."
COMMENT     Sequence Label: 3xDHFR-myc
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(14..43)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(44..517)
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="ISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHT
                     WESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLP
                     KAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
ORIGIN
        1 ggcctctagt ctacagatcc tcttctgaga tgagtttttg ttcccgccgc tccagaatct
       61 caaagcaata gctgtgagag ttctgcgcat cagcatcgtg gaattcgctg aataccgatt
      121 cccagtcatc cggctcgtaa tccgggaaat gggtgtcgcc ttccacttct gcgtcgatat
      181 gcgtcagata cagtttttgc gcttttggca agaactgttc ataaacgcga ccgccgccaa
      241 tcaccatgat ttctggtacg tcaccacacg ccgcgatggc ttcatccacc gacttcaccc
      301 acgttacgcg atcgtccgta cccggttgac tgctgaggat aatatttttg cgtcctggca
      361 acggacgacc gattgattcc caggtatggc ggcccataat cacgggttta tttaaggtgt
      421 tgcgtttaaa ccaggcgaga tcggcaggca ggttccacgg catggcgttt tccatgccga
      481 taacgcgatc taccgctaac gccgcaatca gactgattct agtccgccgc tccagaatct
      541 caaagcaata gctgtgagag ttctgcgcat cagcatcgtg gaattcgctg aataccgatt
      601 cccagtcatc cggctcgtaa tccgggaaat gggtgtcgcc ttccacttct gcgtcgatat
      661 gcgtcagata cagtttttgc gcttttggca agaactgttc ataaacgcga ccgccgccaa
      721 tcaccatgat ttctggtacg tcaccaccac gccggggnnn nntntccccc cnntttcccc
      781 cnngttacnc natcgcccgt acccgggttg aant
//
