LOCUS       sequence-258091-        2601 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Env gene from HIV patient plasma in pcDNA3.1 expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Joshi A, Sedano M, Beauchamp B, Punke EB, Mulla ZD, Meza A, Alozie 
            OK, Mukherjee D, Garg H
  TITLE     HIV-1 Env Glycoprotein Phenotype along with Immune Activation 
            Determines CD4 T Cell Loss in HIV Patients.
  JOURNAL   J Immunol. 2016 Feb 15;196(4):1768-79. doi: 
            10.4049/jimmunol.1501588. Epub 2016 Jan 13.
  PUBMED    26764036
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.1501588"; journalName: "J Immunol"; date: 
            "2016-02-15- 15"; volume: "196"; issue: "4"; pages: "1768-79"
COMMENT     Sequence Label: pHP051Env
FEATURES             Location/Qualifiers
     source          1..2601
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1572..1805
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1990..2034
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             2331..2357
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
ORIGIN
        1 atgagagtga aggggatcag gaagcattgg cagcgctggt ggaaatgggg catgatgctc
       61 cttgggatgt tgatgatctg tgatgccaca gaaaaattgt gggtcacagt atattatggg
      121 gtacctgtgt ggagagaagc aaacgctact ctattttgtg catcagatgc taaagcatat
      181 gatacagagg cacacaatgt ttgggctaca tatgcctgtg tacccacaga ccccaaccca
      241 caagaagtag tattgggaaa tgtgacagaa aattttaata tgtggaaaaa tgacatggtg
      301 gaacagatgc ataaggatat aatcagtcta tgggatgaaa gcctaaagcc atgtgtaaaa
      361 ttaaccccac tctgtgttac cttaaattgc accgatgatt taaatattac tagtaccaca
      421 aataatactg ctggcaccac tggtaataac accactaggg gtaacgaagg agagctaaga
      481 ggagaaatga aaaactgctc tttcaatgtc accacaaaca tgcaagataa gaagcagaaa
      541 gaatatgcgc ttttttataa actggatgta gaaccaatag ataatagtaa taggagttat
      601 aggttgataa gttgtaatac ctcagttgtt acacaggcct gtccaaaagt aaactttgag
      661 ccaattccca tacattattg tgccccggct ggttttgcga ttctaaagtg taataataaa
      721 atatttgatg gaaagggacc atgtacaaat gtcagcacag tacaatgtac acatggaatt
      781 aggccagtag tatcaactca gctgctgtta aatggcagtc tagcagaaaa ggagataatg
      841 attagatcta aaaatttctc ggacaatgct gaaaccataa tagtacagtt gaacaagtct
      901 gtagtaatta attgtataag accccacaac aatacaagaa aaagtataac ttttggacca
      961 gggagggcat tttatgcagc agaccaaata acaggagata taagaaaagc acactgtaac
     1021 attagtgaag cagattggta tgacacttta aaacaggtag ctgcaaaatt aggagaaaaa
     1081 tttaataaaa cagcaatagt ctttgacaat tcctcaggag gggacccaga aattgcaatg
     1141 cacagtttta attgtggagg agaatttttc tactgtaata ctacaccact gtttaataaa
     1201 atttggttta ataggactga ctggtctaat aatactgcag ggtcaaatac cactaacggc
     1261 ataattacac tccaatgcag gataaaacaa attataaaca tgtggcaggg aataggaaaa
     1321 gcaatgtatg cccctcccat cagaggacag attaaatgta catcaaacat tacagggctg
     1381 ctattaacaa gagatggtgg taatccttca aacgcaaccg atgagatctt cagacccata
     1441 gggggaaata tgaaggacaa ttggagaagt gaattatata aatataaagt agtaaaactt
     1501 gaaccaatag gactggcacc caccaaggcg cagagaagag tggtgcagag agaaaaaaga
     1561 gcagtgggac taggagctgt gttccttggg ttcttgggag cagcaggaag cactatgggc
     1621 gcagtgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcaacag
     1681 caaagcaatt tgctgagggc tattgaggcg caacaacata tgttgcaact cacagtctgg
     1741 ggcatcaagc agctccaggc gagaatcctg gctgtggaaa gatacctaaa tgatcaacag
     1801 ctcctgggaa tttggggttg ctctgggaga cacatttgca ccactgctgt gccttgggat
     1861 actagttgga gtaataaatc tctgagtgcc atctgggaga acatgacctg gatggagtgg
     1921 gagagagaga tcagaaatca cacaaaaaca atatattcct taattgaaga atcgcagagc
     1981 cagcaggaaa agaatgaaca ggaattattg gaattggata agtgggcaag tttgtggagt
     2041 tggtttgata taacaaactg gctgtggtat ataagaatat tcataatgat agtaggaggc
     2101 ttagtaggtt taagaatatt tttcactgta ctttctatag tgaatagagt taggcaggga
     2161 tactcaccat tatcgtttca gaccctcctc ccagcagctc ccaggggacc cgacaggccc
     2221 gaaggaatag aagaagacgg tggagaaaga aacagagatg gatccggtcc attagtgaat
     2281 ggattcttag cactcatttg ggtcgacctg cggagcctgt gcctcttcag ctaccaccgc
     2341 ttgagagact tactcttgat tgctgcgagg attgtggaac ttctgggacg cagggggtgg
     2401 gaagccctcc aatattggtg gaacctcctg cagtattgga gtcaggaact aaagaatagt
     2461 gctattagtt tgtttaatgc cacagccata gcagtagctg aagggacaga taggggtata
     2521 gaaatagtgc aaagagctgg tagagctatt cgcaacatac ctagaaggat aagacagggc
     2581 ttagaaagat ctttgctata a
//
