LOCUS       sequence-258108-        4992 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of plant LINC fluorescent fusion protein for cell 
            biology.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   McKenna JF, Gumber HK, Turpin ZM, Jalovec AM, Kartick AC, Graumann 
            K, Bass HW
  TITLE     Maize (Zea mays L.) Nucleoskeletal Proteins Regulate Nuclear 
            Envelope Remodeling and Function in Stomatal Complex Development and
            Pollen Viability.
  JOURNAL   Front Plant Sci. 2021 Feb 17;12:645218. doi: 
            10.3389/fpls.2021.645218. eCollection 2021.
  PUBMED    33679862
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2021-02-17"; volume: "12:645218. doi"; pages: " 
            10.3389/fpls.2021.645218. eCollection 2021"
COMMENT     Sequence Label: MKAKU41ec
FEATURES             Location/Qualifiers
     source          1..4992
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     98..115
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(274..301)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(393..479)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     528..550
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     542..559
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     543..559
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    575..674
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             1394..1417
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1421..1447
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(3123..3222)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3239..3258)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3240..3258)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3263..3279)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3263..3279)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3381..3400)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             3392..4201
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(3459..3478)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      4348..4936
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4837..4856
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
       61 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
      121 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
      181 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatacgcg
      241 taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag gatggccttc
      301 tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc tccgggccgt
      361 tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag agcgttcacc
      421 gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg ttttatttga
      481 tgcctggcag ttccctactc tcgcgttaac gctagcatgg atgttttccc agtcacgacg
      541 ttgtaaaacg acggccagtc ttaagctcgg gccccaaata atgattttat tttgactgat
      601 agtgacctgt tcgttgcaac aaattgatga gcaatgcttt tttataatgc caactttgta
      661 caaaaaagca ggcttcatgg ttagcaaggg agaagaggac aatatggcga taatcaaaga
      721 gttcatgcgg ttcaaggttc acatggaggg ctcggtcaac gggcacgaat tcgagattga
      781 aggagagggc gaaggaaggc catacgaggg cactcagaca gcaaagttga aggtcaccaa
      841 gggcggacca ctccctttcg cttgggacat tctgtctcca cagttcatgt acggctccaa
      901 ggcctatgtg aagcatccag cagacatccc tgattatctc aagctgagct tccctgaagg
      961 cttcaagtgg gagagagtta tgaattttga agatggtggc gtggttaccg tcactcagga
     1021 ctcttcactg caagatggag agttcatcta caaggtgaag cttcgcggta ctaactttcc
     1081 atctgacggc cctgttatgc agaagaagac aatgggttgg gaagcctcca gcgagaggat
     1141 gtatcctgaa gatggtgcac tgaagggcga gatcaagcag agacttaagt tgaaggacgg
     1201 aggtcactac gatgctgagg tcaagactac atataaggcc aagaagccag tgcaattgcc
     1261 tggcgcttac aatgttaaca ttaagctcga catcacttcc cacaatgagg attacacaat
     1321 cgttgagcag tatgaaaggg ccgagggaag acatagcact ggcggcatgg atgagttgta
     1381 caagggtgca ggcgattata aggatgacga tgacaaggct tacccatatg acgtgcctga
     1441 ttacgcagga gctggtatgg caagcctctt cggagctagg agacgcaggt caccagaata
     1501 tgatggtgaa gatgatagat ccggtggcgg aagggcaaag agaagaaggc tgagccctga
     1561 ggaagctgcc gcatctccag cagagcctgg agctgccaca ggtaccagcc atggctggct
     1621 tagcggattt gtctctggtg ctaagagggc catttcttca gtgctcctgt ccagctctcc
     1681 agaggaaact ggctctggag aagacggaga ggtcgaggaa gaggatgacg atgtgtacga
     1741 agagggcatc gatttgaatg agaacgaaga cattcacgat atccatggtg aaattgtccc
     1801 ttattctgag tcaaagctcg ccatcgaaca aatggttatg aaggagacat tctcaagaga
     1861 cgaatgcgat cgcatggttg agctgattaa gtcaagagtc cgcgattcca ctccagaaac
     1921 acacgagtac ggcaagcagg aagagattcc ttccagaaat gcaggaatcg ctcatgactt
     1981 cactggtaca tgccgctccc ttagcaggga tagaaacttc acagagtctg tgccattttc
     2041 atccatgcgc atgaggcctg gccacagctc tccaggattt cctcttcagg cttcaccaca
     2101 attgtgcacc gcagctgtga gggaagccaa gaagtggctt gaagagaaga gacaaggttt
     2161 gggcgttaag ccagaggaca atggctcttg caccctcaac actgatattt tctcatcccg
     2221 cgacgattca gacaagggtt cccctgttga tcttgccaag agctatatga ggtctttgcc
     2281 accttggcag tccccattcc tcggacatca aaagtttgac acctctcctt caaagtacag
     2341 catcagctct actaaggtta ccactaagga ggattatctc tcatcctttt ggactaagct
     2401 ggaagagtct agaattgctc gcatcggcag ctctggagac tcagccgtcg catccaagct
     2461 gtggaattac ggctccaaca gcagactttt cgaaaatgat acctcaatct tttccctggg
     2521 cactgacgag aaggttggag atcctaccaa gactcacaac ggcagcgaaa aggtcgccgc
     2581 aaccgagcca cttggacgct gctctctttt gattacacct accgaagaca ggactgatgt
     2641 gtcatataag gttgccgagg gcaatgacgt gtcatccaca ggaatcacca aggacacaac
     2701 cggtcattca gccgatggca aggcactcac atccgaacca cacattggcg agactcatgt
     2761 taattctgca tcagagtcca tccctaacga tgctgcccca cctactcagt ctaagatgaa
     2821 tggtagcaca aagaagtctc tcgtcaacgg cgtgctggac caaccaaatg ctaactcagg
     2881 attggagagc tctggtaatg attacccttc ctatacaaac tcatccagcg ctatgccacc
     2941 tgccagcacc gagctcattg gttctgcagc tgccgttatc gacgtcgatt ctgctgaaaa
     3001 cggaccaggt actaagccag agcagcctgc caagggcgca tcaagggcta gcaagtctaa
     3061 ggttgtgcca cggggacaga agagggtgct gcggtcggct acacggggac gggctacgtg
     3121 agacccagct ttcttgtaca aagttggcat tataagaaag cattgcttat caatttgttg
     3181 caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag ctgatatccc
     3241 ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc ccgtgtctca
     3301 aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa taaaactgtc
     3361 tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg aaacgtcgag
     3421 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     3481 tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg cgccagagtt
     3541 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     3601 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     3661 tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg tattagaaga
     3721 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     3781 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc
     3841 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     3901 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     3961 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     4021 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     4081 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     4141 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     4201 atcagaattg gttaattggt tgtaacactg gcagagcatt acgctgactt gacgggacgg
     4261 cgcaagctca tgaccaaaat cccttaacgt gagttacgcg tcgttccact gagcgtcaga
     4321 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     4381 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     4441 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     4501 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     4561 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     4621 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     4681 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     4741 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     4801 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     4861 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     4921 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     4981 gccttttgct ca
//
