LOCUS       sequence-258113-        8840 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses NeoR and dsRed..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8840)
  AUTHORS   Kandul NP, Liu J, Hsu AD, Hay BA, Akbari OS
  TITLE     A drug-inducible sex-separation technique for insects.
  JOURNAL   Nat Commun. 2020 Apr 30;11(1):2106. doi: 10.1038/s41467-020-16020-2.
  PUBMED    32355156
REFERENCE   2  (bases 1 to 8840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-04-30"; volume: "11(1):2106. doi"; pages: " 
            10.1038/s41467-020-16020-2"
COMMENT     Sequence Label: Opie2-NeoR
FEATURES             Location/Qualifiers
     source          1..8840
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     60..77
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    194..215
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(230..235,236..253,254..260)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    268..284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     273..295
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     292..308
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     292..308
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     repeat_region   489..523
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     polyA_signal    1212..1333
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(1454..2128,2129..2131,2132..2134))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(1516..1539)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(1714..1733)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1931..1951
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     promoter        complement(2145..2736)
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     enhancer        2740..3222
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     CDS             3785..4579
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             3785..4579
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3839..3858)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4449..4468
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    4596..4730
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   5766..5828
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(6532..6549)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6532..6548)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6541..6563)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6757..6776)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6876..6898
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6936..6954)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7022..7126
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7127..7195,7196..7987)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7345..7364)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8158..8746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8647..8666
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
       61 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
      121 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      181 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
      241 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
      301 gctatgacat gattacgaat tcgagctcgg tacccgggga tcctctagag tcgacgctcg
      361 cgcgacttgg tttgccattc tttagcgcgc gtcgcgtcac acagcttggc cacaatgtgg
      421 tttttgtcaa acgaagattc tatgacgtgt ttaaagttta ggtcgagtaa agcgcaaatc
      481 ttttttaacc ctagaaagat agtctgcgta aaattgacgc atgcattctt gaaatattgc
      541 tctctctttc taaatagcgc gaatccgtcg ctgtgcattt aggacatctc agtcgccgct
      601 tggagctccc gtgaggcgtg cttgtcaatg cggtaagtgt cactgatttt gaactataac
      661 gaccgcgtga gtcaaaatga cgcatgatta tcttttacgt gacttttaag atttaactca
      721 tacgataatt atattgttat ttcatgttct acttacgtga taacttatta tatatatatt
      781 ttcttgttat agatatcgtg actaatatat aataaaatgg gtagttcttt agacgatgag
      841 catatcctct ctgctcttct gcaaagcgat gacgagcttg ttggtgagga ttctgacagt
      901 gaaatatcag atcacgtaag tgaagatgac gtccagagcg atacagaaga agcgtttata
      961 gatgaggtac atgaagtgca gccaacgtca agcggtagtg aaatattaga cgaacaaaat
     1021 gttattgaac aaccaggttc ttcattggct tctaacagaa tcttgacctt gccacagagg
     1081 actattagag gtaagaataa acattgttgg tcaacttcaa agtccacgag gcgtagccga
     1141 gtctctgcac tgaacattgt cagatccgag atcggccggc ctaggcgcgc cgtacgcgta
     1201 tcgataagct ttaagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     1261 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     1321 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     1381 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatct
     1441 agagtcgcgg ccgctacagg aacaggtggt ggcggccctc ggtgcgctcg tactgctcca
     1501 cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggagtccacg tagtagtagc
     1561 cgggcagctg cacgggcttc ttggccatgt agatggactt gaactccacc aggtagtggc
     1621 cgccgtcctt cagcttcagg gccttgtgga tctcgccctt cagcacgccg tcgcgggggt
     1681 acaggcgctc ggtggaggcc tcccagccca tggtcttctt ctgcattacg gggccgtcgg
     1741 aggggaagtt cacgccgatg aacttcacct tgtagatgaa gcagccgtcc tgcagggagg
     1801 agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc tcccacttga
     1861 agccctcggg gaaggacagc ttcttgtagt cggggatgtc ggcggggtgc ttcacgtaca
     1921 ccttggagcc gtactggaac tggggggaca ggatgtccca ggcgaagggc agggggccgc
     1981 ccttggtcac cttcagcttc acggtgttgt ggccctcgta ggggcggccc tcgccctcgc
     2041 cctcgatctc gaactcgtgg ccgttcacgg tgccctccat gcgcaccttg aagcgcatga
     2101 actccttgat gacgttcttg gaggagcgca ccatctcgag gtcacttggt tgttcacgat
     2161 cttgtcgccg ccagtgtcaa cttgcaactg aaacaatatc caacatgaac gtcaatttat
     2221 actgccctaa tggcgaacac gataacaata tttcttttat tatgccctct aaaaccaacg
     2281 cggttatcgt ttatttattc aaattagata tagaacatcc gccgacatac aatgttaatg
     2341 caaaaacgcg tttggtgagc ggatacgaaa acagtcggcc gataaacatt aatctgaggt
     2401 cgataacacc gtccttgaac ggaacacgag gagcgtacgt gatcagctgc attcgcgcgc
     2461 cgcgccttta tcgagattta tttgcataca acaagtacac tgcgccgttg ggatttgtgg
     2521 taacgcgcac acatgcagag ctgcaagtgt ggcacatttt gtctgtgcgc aaaacctttg
     2581 aagccaaaag tacgaggtcc gttacgggca tgctagcgca cacggacaat ggacccgaca
     2641 aattctacgc caaggattta atgataatgt cgggcaacgt atccgttcat tttatcaata
     2701 acctacaaaa atgtcgcgcg catcacaaag acatcgacgc gcgtagaatt ctacccgtaa
     2761 agcgagttta gttatgagcc atgtgcaaaa catgacatca gcttttattt ttataacaaa
     2821 tgacatcatt tcttgattgt gttttacacg tagaattcta ctcgtaaagc gagttcagtt
     2881 ttgaaaaaca aatgacatca tctttttgat tgtgctttac aagtagaatt ctacccgtaa
     2941 atcaagttcg gttttgaaaa acaaatgagt catattgtat gatatcatat tgcaaaacaa
     3001 atgactcatc aatcgatcgt gcgttacacg tagaattcta ctcgtaaagc gagtttatga
     3061 gccgtgtgca aaacatgaca tcatctcgat ttgaaaaaca aatgacatca tccactgatc
     3121 gtgcgttaca agtagaattc tactcgtaaa gccagttcgg ttatgagccg tgtgcaaaac
     3181 atgacatcag cttatgactc atacttgatt gtgttttacg cggtttaaac gcgcccatac
     3241 tcggtggcct ccccaccacc aacttttttg cactgcaaaa aaacacgctt ttgcacgcgg
     3301 gcccatacat agtacaaact ctacgtttcg tagactattt tacataaata gtctacaccg
     3361 ttgtatacgc tccaaataca ctaccacaca ttgaaccttt ttgcagtgca aaaaagtacg
     3421 tgtcggcagt cacgtaggcc ggccttatcg ggtcgcgtcc tgtcacgtac gaatcacatt
     3481 atcggaccgg acgagtgttg tcttatcgtg acaggacgcc agcttcctgt gttgctaacc
     3541 gcagccggac gcaactcctt atcggaacag gacgcgcctc catatcagcc gcgcgttatc
     3601 tcatgcgcgt gaccggacac gaggcgcccg tcccgcttat cgcgcctata aatacagccc
     3661 gcaacgatct ggtaaacaca gttgaacagc atctgttaca gcgacacaac atgagccggt
     3721 ccaacaacgc caacgcgccc acgccatcca accgccgccg caacctgtct ctggtgttaa
     3781 ttaaatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
     3841 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
     3901 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
     3961 actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
     4021 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
     4081 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
     4141 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
     4201 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
     4261 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgagcatgcc
     4321 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
     4381 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
     4441 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
     4501 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
     4561 tcttgacgag ttcttctgaa gatcttcgac ctcgaaactt gtttattgca gcttataatg
     4621 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     4681 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc atgtcgaggg
     4741 ggggcccgat ttaaataaac cccgggcgag ctcgaattaa ccattgtggg aaccgtgcga
     4801 tcaaacaaac gcgagatacc ggaagtactg aaaaacagtc gctccaggcc agtgggaaca
     4861 tcgatgtttt gttttgacgg accccttact ctcgtctcat ataaaccgaa gccagctaag
     4921 atggtatact tattatcatc ttgtgatgag gatgcttcta tcaacgaaag taccggtaaa
     4981 ccgcaaatgg ttatgtatta taatcaaact aaaggcggag tggacacgct agaccaaatg
     5041 tgttctgtga tgacctgcag taggaagacg aataggtggc ctatggcatt attgtacgga
     5101 atgataaaca ttgcctgcat aaattctttt attatataca gccataatgt cagtagcaag
     5161 ggagaaaagg ttcaaagtcg caaaaaattt atgagaaacc tttacatgag cctgacgtca
     5221 tcgtttatgc gtaagcgttt agaagctcct actttgaaga gatatttgcg cgataatatc
     5281 tctaatattt tgccaaatga agtgcctggt acatcagatg acagtactga agagccagta
     5341 atgaaaaaac gtacttactg tacttactgc ccctctaaaa taaggcgaaa ggcaaatgca
     5401 tcgtgcaaaa aatgcaaaaa agttatttgt cgagagcata atattgatat gtgccaaagt
     5461 tgtttctgac tgactaataa gtataatttg tttctattat gtataagtta agctaattac
     5521 ttattttata atacaacatg actgttttta aagtacaaaa taagtttatt tttgtaaaag
     5581 agagaatgtt taaaagtttt gttactttat agaagaaatt ttgagttttt gttttttttt
     5641 aataaataaa taaacataaa taaattgttt gttgaattta ttattagtat gtaagtgtaa
     5701 atataataaa acttaatatc tattcaaatt aataaataaa cctcgatata cagaccgata
     5761 aaacacatgc gtcaatttta cgcatgatta tctttaacgt acgtcacaat atgattatct
     5821 ttctagggtt aaataatagt ttctaatttt tttattattc agcctgctgt cgtgaatacc
     5881 gtatatctca acgctgtctg tgagattgtc gtattctagc ctttttagtt tttcgctcat
     5941 cgacttgata ttgtccgaca cattttcgtc gatttgcgtt ttgatcaaag acttgagcag
     6001 agacacgtta atcaactgtt caaattgatc catattaacg atatcaaccc gatgcgtata
     6061 tggtgcgtaa aatatatttt ttaaccctct tatactttgc actctgcgtt aatacgcgtt
     6121 cgtgtacaga cgtaatcatg ttttcttttt tggataaaac tcctactgag tttgacctca
     6181 tattagaccc tcacaagttg caaaacgtgg cattttttac caatgaagaa tttaaagtta
     6241 ttttaaaaaa tttcatcaca gatttaaaga agaaccaaaa attaaattat ttcaacagtt
     6301 taatcgacca gttaatcaac gtgtacacag acgcgtcggc aaaaaacacg cagcccgacg
     6361 tgttggctaa aattattaaa tcaacttgtg ttatagtcac ggatttgccg tccaacgtgt
     6421 tcctcaaaaa gttgaagacc aacaagttta cggacactat taattatttg attttgcccc
     6481 acttcatttt gtgggatcac aattttgtta tatttttaaa caaagcttgg cactggccgt
     6541 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     6601 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     6661 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
     6721 gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg atgccgcata
     6781 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     6841 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     6901 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     6961 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     7021 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     7081 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     7141 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     7201 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     7261 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     7321 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     7381 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     7441 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7501 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7561 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7621 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7681 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7741 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7801 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7861 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7921 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7981 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     8041 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     8101 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     8161 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     8221 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     8281 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     8341 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     8401 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     8461 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8521 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8581 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8641 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8701 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8761 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8821 atcccctgat tctgtggata
//
