LOCUS       sequence-258116-        8891 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes Alpha Sarcoglycan 3xFLAG tagged protein (Therapeutic gene) 
            to cure LGMD2D and luciferase enzyme for in vivo live imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8891)
  AUTHORS   Guha TK, Pichavant C, Calos MP
  TITLE     Plasmid-Mediated Gene Therapy in Mouse Models of Limb Girdle 
            Muscular Dystrophy.
  JOURNAL   Mol Ther Methods Clin Dev. 2019 Oct 14;15:294-304. doi: 
            10.1016/j.omtm.2019.10.002. eCollection 2019 Dec 13.
  PUBMED    31890729
REFERENCE   2  (bases 1 to 8891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther 
            Methods Clin Dev."; date: "2019-10-14"; volume: "15:294-304. doi"; 
            pages: " 10.1016/j.omtm.2019.10.002. eCollection 2019 Dec 13"
COMMENT     Sequence Label: pgWIZ-SGCAgl
FEATURES             Location/Qualifiers
     source          1..8891
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..34
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     enhancer        52..431
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        432..635
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     586..606
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     632..656
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          771..1596
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1620..1629
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2202..2213
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             2901..2957
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2988..3704
                     /codon_start=1
                     /product="green fluorescent protein"
                     /label=GFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(3021..3049)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     3644..3664
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     CDS             3726..3782
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3783..5435
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(3849..3867)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    5468..5692
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6115..6132)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6286..6874)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6366..6385)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7416..8231)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7416..8231)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8139..8158
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8223..8242
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     8509..8527
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8565..8587)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8687..8706
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gtgccagggc gtgcccttgg gctccccggg cgcgtactcc accgccatgt tgacattgat
       61 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
      121 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
      181 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
      241 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
      301 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
      361 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      421 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      481 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      541 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      601 ggcgtgtacg gtgggaggtc tatataagca gagctcgttt agtgaaccgt cagatcgcct
      661 ggagacgcca tccacgctgt tttgacctcc atagaagaca ccgggaccga tccagcctcc
      721 gcggccggga acggtgcatt ggaacgcgga ttccccgtgc caagagtgac gtaagtaccg
      781 cctatagact ctataggcac acccctttgg ctcttatgca tgctatactg tttttggctt
      841 ggggcctata cacccccgct tccttatgct ataggtgatg gtatagctta gcctataggt
      901 gtgggttatt gaccattatt gaccactccc ctattggtga cgatactttc cattactaat
      961 ccataacatg gctctttgcc acaactatct ctattggcta tatgccaata ctctgtcctt
     1021 cagagactga cacggactct gtatttttac aggatggggt cccatttatt atttacaaat
     1081 tcacatatac aacaacgccg tcccccgtgc ccgcagtttt tattaaacat agcgtgggat
     1141 ctccacgcga atctcgggta cgtgttccgg acatgggctc ttctccggta gcggcggagc
     1201 ttccacatcc gagccctggt cccatgcctc cagcggctca tggtcgctcg gcagctcctt
     1261 gctcctaaca gtggaggcca gacttaggca cagcacaatg cccaccacca ccagtgtgcc
     1321 gcacaaggcc gtggcggtag ggtatgtgtc tgaaaatgag cgtggagatt gggctcgcac
     1381 ggctgacgca gatggaagac ttaaggcagc ggcagaagaa gatgcaggca gctgagttgt
     1441 tgtattctga taagagtcag aggtaactcc cgttgcggtg ctgttaacgg tggagggcag
     1501 tgtagtctga gcagtactcg ttgctgccgc gcgcgccacc agacataata gctgacagac
     1561 taacagactg ttcctttcca tgggtctttt ctgcagtcac cgtcgtcgac acgggcgcgg
     1621 ccaccatggg cactagtatg gctgagacac tcttctggac tcctctcctc gtggttctcc
     1681 tggcagggct gggggacacc gaggcccagc agaccacgct acacccactt gtgggccgtg
     1741 tctttgtgca caccttggac catgagacgt ttctgagcct tcctgagcat gtcgctgtcc
     1801 cacccgctgt ccacatcacc taccacgccc acctccaggg acacccagac ctgccccggt
     1861 ggctccgcta cacccagcgc agcccccacc accctggctt cctctacggc tctgccaccc
     1921 cagaagatcg tgggctccag gtcattgagg tcacagccta caatcgggac agctttgata
     1981 ccactcggca gaggctggtg ctggagattg gggacccaga aggccccctg ctgccatacc
     2041 aagccgagtt cctggtgcgc agccacgatg cggaggaggt gctgccctca acacctgcca
     2101 gccgcttcct ctcagccttg gggggactct gggagcccgg agagcttcag ctgctcaacg
     2161 tcacctctgc cttggaccgt gggggccgtg tcccccttcc cattgagggc cgaaaagaag
     2221 gggtatacat taaggtgggt tctgcctcac ctttttctac ttgcctgaag atggtggcat
     2281 cccccgatag ccacgcccgc tgtgcccagg gccagcctcc acttctgtct tgctacgaca
     2341 ccttggcacc ccacttccgc gttgactggt gcaatgtgac cctggtggat aagtcagtgc
     2401 cggagcctgc agatgaggtg cccaccccag gtgatgggat cctggagcat gacccgttct
     2461 tctgcccacc cactgaggcc ccagaccgtg acttcttggt ggatgctctg gtcaccctcc
     2521 tggtgcccct gctggtggcc ctgcttctca ccttgctgct ggcctatgtc atgtgctgcc
     2581 ggcgggaggg aaggctgaag agagacctgg ctacctccga catccagatg gtccaccact
     2641 gcaccatcca cgggaacaca gaggagctgc ggcagatggc ggccagccgc gaggtgcccc
     2701 ggccactctc caccctgccc atgttcaatg tgcacacagg tgagcggctg cctccccgcg
     2761 tggacagcgc ccaggtgccc ctcattctgg accagcacgc tagcaccgga tccgactaca
     2821 aggaccatga cggcgattac aaggaccatg atatagatta caaagacgac gataagggtt
     2881 ctgcggccgc cctaggctct gccacgaact tctctctgtt aaagcaagca ggagacgtgg
     2941 aagaaaaccc cggtcctatg gtgggcctcg acggtcctgg cgcccatatg gtgagtaaag
     3001 gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg
     3061 ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc
     3121 ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt
     3181 taacttatgg tgttcaatgc ttttcaagat acccagatca catgaaacag catgactttt
     3241 tcaagagtgc catgcccgaa ggttatgtcc aggaaagaac tatatttttc aaagatgacg
     3301 ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg
     3361 agttaaaagg tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca
     3421 actataactc acacaatgta tacatcatgg cagacaaaca aaagaatgga atcaaagtta
     3481 acttcaaaat tagacacaac attgaagatg gaagcgttca actagcagac cattatcaac
     3541 aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtccacac
     3601 aatctgccct ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg
     3661 taacagctgc tgggattaca catggcatgg atgaactata caaagggtcc gctaggggct
     3721 ccggagccac gaacttctct ctgttaaagc aagcaggaga cgtggaagaa aaccccggtc
     3781 ctatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg
     3841 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa
     3901 ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt
     3961 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
     4021 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
     4081 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca
     4141 tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc
     4201 aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg
     4261 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg
     4321 attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg
     4381 gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct
     4441 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg
     4501 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat
     4561 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt
     4621 acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc
     4681 tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct
     4741 ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat
     4801 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg
     4861 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga
     4921 aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg
     4981 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt
     5041 ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt
     5101 ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc
     5161 aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac
     5221 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg
     5281 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg
     5341 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca
     5401 taaaggccaa gaagggcgga aagatcgccg tgtaatgaga tctgtgtgtt ggttttttgt
     5461 ggatctgctg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     5521 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     5581 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     5641 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat gggtacctct
     5701 ctctctctct ctctctctct ctctctctct ctctctctgg tacctctctc tctctctctc
     5761 tctctctctc tctctctctc tggtacccag gtgctgaaga attgacccgg ttcctcctgg
     5821 gccagaaaga agcaggcaca tccccttctc tgtgacacac cctgtccacg cccctggttc
     5881 ttagttccag ccccactcat aggacactca tagctcagga gggctccgcc ttcaatccca
     5941 cccgctaaag tacttggagc ggtctctccc tccctcatca gcccaccaaa ccaaacctag
     6001 cctccaagag tgggaagaaa ttaaagcaag ataggctatt aagtgcagag ggagagaaaa
     6061 tgcctccaac atgtgaggaa gtaatgagag aaatcataga atttcttccg cttcctcgct
     6121 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     6181 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg
     6241 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg
     6301 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg
     6361 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac
     6421 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca
     6481 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt
     6541 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc
     6601 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag
     6661 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac
     6721 tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt
     6781 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa
     6841 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg
     6901 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa
     6961 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     7021 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     7081 gatctgtcta tttcgttcat ccatagttgc ctgactcggg gggggggggc gctgaggtct
     7141 gcctcgtgaa gaaggtgttg ctgactcata ccaggcctga atcgccccat catccagcca
     7201 gaaagtgagg gagccacggt tgatgagagc tttgttgtag gtggaccagt tggtgatttt
     7261 gaacttttgc tttgccacgg aacggtctgc gttgtcggga agatgcgtga tctgatcctt
     7321 caactcagca aaagttcgat ttattcaaca aagccgccgt cccgtcaagt cagcgtaatg
     7381 ctctgccagt gttacaacca attaaccaat tctgattaga aaaactcatc gagcatcaaa
     7441 tgaaactgca atttattcat atcaggatta tcaataccat atttttgaaa aagccgtttc
     7501 tgtaatgaag gagaaaactc accgaggcag ttccatagga tggcaagatc ctggtatcgg
     7561 tctgcgattc cgactcgtcc aacatcaata caacctatta atttcccctc gtcaaaaata
     7621 aggttatcaa gtgagaaatc accatgagtg acgactgaat ccggtgagaa tggcaaaagc
     7681 ttatgcattt ctttccagac ttgttcaaca ggccagccat tacgctcgtc atcaaaatca
     7741 ctcgcatcaa ccaaaccgtt attcattcgt gattgcgcct gagcgagacg aaatacgcga
     7801 tcgctgttaa aaggacaatt acaaacagga atcgaatgca accggcgcag gaacactgcc
     7861 agcgcatcaa caatattttc acctgaatca ggatattctt ctaatacctg gaatgctgtt
     7921 ttcccgggga tcgcagtggt gagtaaccat gcatcatcag gagtacggat aaaatgcttg
     7981 atggtcggaa gaggcataaa ttccgtcagc cagtttagtc tgaccatctc atctgtaaca
     8041 tcattggcaa cgctaccttt gccatgtttc agaaacaact ctggcgcatc gggcttccca
     8101 tacaatcgat agattgtcgc acctgattgc ccgacattat cgcgagccca tttataccca
     8161 tataaatcag catccatgtt ggaatttaat cgcggcctcg agcaagacgt ttcccgttga
     8221 atatggctca taacacccct tgtattactg tttatgtaag cagacagttt tattgttcat
     8281 gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac aacgtggctt
     8341 tccccccccc cccattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8401 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8461 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
     8521 acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     8581 ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     8641 ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag
     8701 attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     8761 taccgcatca gattggctat tggccattgc atacgttgta tccatatcat aatatgtaca
     8821 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actaggcgcg
     8881 ccgcggtgcg g
//
