LOCUS       sequence-258120-        8818 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene targeting in marmoset cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Yoshimatsu S, Okahara J, Sone T, Takeda Y, Nakamura M, Sasaki E, 
            Kishi N, Shiozawa S, Okano H
  TITLE     Robust and efficient knock-in in embryonic stem cells and 
            early-stage embryos of the common marmoset using the CRISPR-Cas9 
            system.
  JOURNAL   Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1038/s41598-018-37990-w.
  PUBMED    30728412
REFERENCE   2  (bases 1 to 8818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-6"; volume: "9(1):1528. doi"; pages: " 
            10.1038/s41598-018-37990-w"
COMMENT     Sequence Label: pKI-cjPLP1e2-P15L
FEATURES             Location/Qualifiers
     source          1..8818
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..23
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15..32
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     16..32
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    73..93
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2095..2128
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2128..2152
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2159..2658
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2194..2217)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2591..2610
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2682..3278
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2682..2701)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3178..3198
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3285..4280
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     protein_bind    complement(4646..4670)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    4670..4703
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(6153..6173)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6234..6250)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6234..6250)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6247..6269)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6258..6274
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6282..6288,6289..6306,6307..6312))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6327..6348
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6465..6482)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6636..7224)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6716..6735)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7395..8186,8187..8255))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8018..8037
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8256..8360)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8428..8446
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8484..8506)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8606..8625
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cccagtcacg acgttgtaaa acgacggcca gtgccaagct tgcatgcctg caggtcggga
       61 tatctgaatt atcaactttg tataataaag ttgaaggtgc actccatatg acatcttaga
      121 gcatgggact agtacttgga acacagctgc tttcagagtc tgtgacttct tgtgtgcctt
      181 tcctgtttct cagcaacact ggcatggggc ctgggatacc aggtctgggg atctcaggga
      241 ctcttagcac tttaagacac atgtgttccc aggccctggt gtgttctcct agtgccagaa
      301 agatgtttca tgctttgctg actttgtata aagtctgttt gtagctgttt tgacagaatc
      361 tcagtgtaga actgaaggtg ggcacgttag ccaagctgca ctataggagg acaaaaccac
      421 catgcaaagt gtccaaaaat tattaagccc atgtttttat tatagtgagt aaagcagtct
      481 tcccattttc atttcttgtc ctatgctagc tccctcctgt ttggactgct cctctcatat
      541 gtaaactaag aagaatcaag cattctttgc aacaaataca cacgatgctc aaaaatatcc
      601 aggagcatgc aatttccaaa gtttccttca cctggaatgt tcttcaggct aaaatcctgt
      661 ctgacaatac cagcatctct ggcctgcact catcccttcc tggacctgca agtgcatcta
      721 ccctctgtta ccacttactt ggctgcctaa attgttagtt taaaatatta ggtctatcta
      781 gctaaatctt cctcaggaat ctaaagactc ccataaggca gagtctgtgt cttttctctc
      841 ttcatatccc atataacacc cactataatg ctgggcatat agtgagtatt tcataaatag
      901 ttgatgaatg actaaaataa gcaagcaaac aaacagacca gagcaataag gaaggaggga
      961 ctgatttcat gatctctctg gcttgctatt tgaattgctg aattattatt gtttgttaaa
     1021 tatatttttt agaaattctg ggaattaaag gtaaggattt tttttttctt tccttttttt
     1081 tttttttctt gagacagaat cttgctcttg tcacccaggc tggagtgcaa tggcgcaatc
     1141 ttggctcact gcaacctcca cttcctgggt tcaagagatt ttcctgcctc agccccctga
     1201 gtagctgaga ttacaggcat gtgccaccat gcctggctaa ttttcatatc tttagtagag
     1261 atggtgtttc accatgttgg ccaagctggt ctcgaactcc tgacctcagg taatcctcct
     1321 tagcctccca aagtgctggg attacaggtg tgagccacgg cgccaggcca aggatttatt
     1381 ttcaatgtaa ggattctggg tcgatctcac atgctgaaag ccaaaaccct tagtttctcc
     1441 tgcttcttga ctctggggtg cccactatct ccaagcctgt aagtgcagag cctggctggc
     1501 ctggaagaga gctttgagtg gcatgagcta cctactggat gtgcctgact gtttcccctt
     1561 cttcttcccc aggcttgtta gaatgttgcg ctcgttgctt agttggagct ctctttgctt
     1621 ccctggtggc cactggattg tgtttctttg gggtggcact gttctgtggc tgtggacatg
     1681 aagccctcac tggcacagaa aagctaattg aaacctattt ctccaaaaac tatcaagact
     1741 atgagtatct catcaatgtg taagtacttg ccctcccaca cagacccatc tttttttttt
     1801 tccctctctc catcctggag atagagtact ctaatatctt agtaactagg ggatgggtgg
     1861 agccagacag gattcctgag tcttccctct gtgcagattt ctcctgttcc acattcaaat
     1921 cctcaaatgt ggggaaattg ccagattcag aaaggagagg tctgcctgcc tgcctgcctg
     1981 ccagcctttc ccagcccctg ttttctcaca cgtgctctga cttctgctag gtgtggttaa
     2041 tattgcccaa gttggagcct ccacagtggt aggtatggag aacccaagag aggcataact
     2101 tcgtataatg tatgctatac gaagttatca agtttgtaca aaaaagcaga attctaccgg
     2161 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     2221 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     2281 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     2341 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     2401 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     2461 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     2521 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     2581 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     2641 tcatctccgg gcctttcgac cgatcatcaa gcttgatcct catgaccgag tacaagccca
     2701 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     2761 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     2821 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     2881 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     2941 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     3001 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     3061 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     3121 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     3181 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     3241 gaccgcgcac ctggtgcatg acccgcaagc ccggtgccgg atccatgccc acgctactgc
     3301 gggtttatat agacggtccc cacgggatgg ggaaaaccac caccacgcaa ctgctggtgg
     3361 ccctgggttc gcgcgacgat atcgtctacg tacccgagcc gatgacttac tggcgggtgc
     3421 tgggggcttc cgagacaatc gcgaacatct acaccacaca acaccgcctc gaccagggtg
     3481 agatatcggc cggggacgcg gcggtggtaa tgacaagcgc ccagataaca atgggcatgc
     3541 cttatgccgt gaccgacgcc gttctggctc ctcatatcgg gggggaggct gggagctcac
     3601 atgccccgcc cccggccctc accctcatct tcgaccgcca tcccatcgcc gccctcctgt
     3661 gctacccggc cgcgcggtac cttatgggca gcatgacccc ccaggccgtg ctggcgttcg
     3721 tggccctcat cccgccgacc ttgcccggca ccaacatcgt gcttggggcc cttccggagg
     3781 acagacacat cgaccgcctg gccaaacgcc agcgccccgg cgagcggctg gacctggcta
     3841 tgctggctgc gattcgccgc gtttacgggc tacttgccaa tacggtgcgg tatctgcagt
     3901 gcggcgggtc gtggcgggag gactggggac agctttcggg gacggccgtg ccgccccagg
     3961 gtgccgagcc ccagagcaac gcgggcccac gaccccatat cggggacacg ttatttaccc
     4021 tgtttcgggc ccccgagttg ctggccccca acggcgacct gtataacgtg tttgcctggg
     4081 ccttggacgt cttggccaaa cgcctccgtt ccatgcacgt ctttatcctg gattacgacc
     4141 aatcgcccgc cggctgccgg gacgccctgc tgcaacttac ctccgggatg gtccagaccc
     4201 acgtcaccac ccccggctcc ataccgacga tatgcgacct ggcgcgcacg tttgcccggg
     4261 agatggggga ggctaactga gctctagctt atcgactcgc cgatagtgga aaccgacgcc
     4321 ccagcactcg tccgagggca aaggaatagt cgagaaattg atgatctatt aaacaataaa
     4381 gatgtccaca tggaagtttt tcctgtcata ctttgttaag aagggtgaga acagagtacc
     4441 tacattttga atggaaggat tggagctacg ggggtggggg tggggtggga ttagataaat
     4501 gcctgctctt tactgaaggc tctttactat tgctttatga taatgtttca tagttggata
     4561 tcataattta aacaagcaaa accaaattaa gggccagctc attcctccca ctcatgatct
     4621 atagatcctc tagagtcgag ggctgcagca gctttcttgt acaaagtgga taacttcgta
     4681 taatgtatgc tatacgaagt tattctaagc ctctcctgtt cctagaacaa cttaggctcc
     4741 tgttccttca cccacctttc ttcttaccta gctccccact cctgtgaggt ggcatgtctg
     4801 aaggatcggg cagggagagt agggccctgg ttctccaggt cacagggtaa gcaagatgtg
     4861 gcagaaagat catatgtttc tggtcacata cactgtgtct tcacttattt caacaaagat
     4921 agggtaaaag tatgcaggag gaacctggtg attcctctag agaatcccta gctttgttag
     4981 ggtgcctgct ctggcgtata ccacatttat gtgagtaaag gagctgggtc ttcaattaat
     5041 acgattcctt cgtctctttc atctccctgt tactgcagga tccatgcctt ccagtatgtc
     5101 atctatggaa ctgcctcttt cttcttcctt tatggggccc tcctgctggc tgagggcttc
     5161 tacaccaccg gcgcagtcag gcagatcttt ggcgactaca agaccaccat ctgcggcaag
     5221 ggcctgagcg caacggtaac agggggccag aaggggaggg gttccagagg ccaacatcaa
     5281 gctcattctt tggagcgggt gtgtcattgt ttgggaaaat ggctaggaca tcccgacaag
     5341 gtgatcatcc tcaggatttt gtggcaataa caaggggtgg gggaaaattg ggcgtgagtc
     5401 tgtggcctcg tccccaccca aggctgggtc ctctctaggg gcccggcatt tgagtgagga
     5461 agcgatggct gcagccgaac gagaagatca ggaagaacgt ggtgcccagc tggcttagcc
     5521 tcacctttca aaggttccct aagcaaattt cttttcaaaa tggaaagcat gagttatgta
     5581 ggatgctttg tacaatcaga ccatttctaa gccacctgtt ggtatccctt tactcccttc
     5641 ctagcaggga ccacaagagt ggatataact ggacaagagt ctaaaatact gctcgtgaga
     5701 ttgagacttg gacacctgat ctgagaagga ggattgataa taaaaattaa ataattgact
     5761 ccaaggtgaa atttaccata ttccgggatc ctactctgtt ggaagtccca gagggaattg
     5821 gagtagatct gcatgttgaa tgttttatca ttgtctgagg gttatccatc tgtgctttga
     5881 gtggggtgca cattggtttt cagtatctgg agttaaagat cctttgaggg ctgaacatgt
     5941 gcaggttcta gccatgtctt tttgtggcca gcagtcagct tagaaaggtt ttgtatatga
     6001 aattttaatc tcctattggc tttgttcaat gactagggaa caaaaatgtt cccgtggcaa
     6061 ggaacatttt ctaagctcag cctaaggcac aaaacggcac gactttcttt caagatctat
     6121 tttgatttct ttacacctta tctgcccaga aacaactttt ctatacaaag ttgatagctt
     6181 gatatcaata ctctagagga tccccgggta ccgagctcga attcgtaatc atggtcatag
     6241 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     6301 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     6361 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     6421 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     6481 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6541 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6601 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6661 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6721 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6781 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     6841 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     6901 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     6961 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7021 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     7081 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7141 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7201 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7261 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     7321 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     7381 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     7441 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     7501 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     7561 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     7621 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     7681 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     7741 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     7801 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     7861 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     7921 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     7981 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     8041 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     8101 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     8161 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     8221 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     8281 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     8341 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc
     8401 attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg
     8461 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     8521 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     8581 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     8641 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg
     8701 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     8761 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggtttt
//
