LOCUS       sequence-258122-       12389 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses PSD95 fused to Teal fluorescent protein upon coexpression 
            with the recombinase flippase (Flp).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12389)
  AUTHORS   Subramanian J, Michel K, Benoit M, Nedivi E
  TITLE     CPG15/Neuritin Mimics Experience in Selecting Excitatory Synapses 
            for Stabilization by Facilitating PSD95 Recruitment.
  JOURNAL   Cell Rep. 2019 Aug 6;28(6):1584-1595.e5. doi: 
            10.1016/j.celrep.2019.07.012.
  PUBMED    31390571
REFERENCE   2  (bases 1 to 12389)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.07"; journalName: "Cell Rep"; date: 
            "2019-08-6- 6"; volume: "28"; issue: "6"; pages: "1584-1595.e5"
COMMENT     Sequence Label: pFudioFRTPSD-95TealW
FEATURES             Location/Qualifiers
     source          1..12389
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2611..3821
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3658..3677
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    3862..3895
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             complement(3988..4698)
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVKHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
     CDS             complement(6859..6888)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      6921..6930
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(6938..6971)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    7081..7669
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7134..7154)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7552..7563)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7672..7688)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7673..7689)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8194..8374
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8400..8417)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8406..8630
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8676..9104
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8763..8782)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8973..8994
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9113..9133)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9118..9447
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9298..9433
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9360..9379
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9495..9542
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9561..9935
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10065..10186
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10102..10121)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10156..10175
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10235..10251)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10235..10251)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10248..10270)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10259..10275
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10283..10289,10290..10307,10308..10313))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10328..10349
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10466..10483)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10637..11225)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10717..10736)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11396..12187,12188..12256))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12019..12038
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12257..12361)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agggtgcagc ggcctccgcg ccgggttttg gcgcctcccg
     2641 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc aggagcgttc
     2701 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag
     2761 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg
     2821 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg
     2881 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac
     2941 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc
     3001 gtcacttggt gagttgcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct
     3061 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag
     3121 gttgccctga actgggggtt ggggggagcg cacaaaatgg cggctgttcc cgagtcttga
     3181 atggaagacg cttgtaaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg
     3241 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga
     3301 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc
     3361 gggtttgtcg tctggttgcg ggggcggcag ttatgcggtg ccgttgggca gtgcacccgt
     3421 acctttggga gcgcgcgcct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg
     3481 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg
     3541 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg
     3601 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga
     3661 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg
     3721 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaat
     3781 tgtccgctaa attctggccg tttttggctt ttttgttaga cgaagcttgg gctgcaggtc
     3841 gactctagag gatcctccgg agaagttcct attctctaga aagtatagga acttctgcag
     3901 aatggtagct ggattgtagc tgctattagc aatatgaaac ctcttagaag ttcctattct
     3961 tcaaaaggta taggaacttc ggcgcgttta cttgtacagc tcgtccatgc cgtcggtgga
     4021 gttgcgggcc acggcgctct cgtaaacggt caccttgttg tagtccttgt cgtggttcag
     4081 gatctcgatg cggtggtcca caaagtgata gtcgggcagc ttcaccgcct tcttggccct
     4141 gtagatggtc ttgaagtcaa cgcggtggtg gccgccgccc tccagcagca gcttgtgctt
     4201 gacgtcgccc ttcagcacgc cgtcgcgcac gtacatcctc tcggtggagg cgtcccagcc
     4261 ggtggtcttc ttctgcatca cggggccgtt gggggggaag ttctcgccct tgaggtgtat
     4321 ctcgtagatg aaggagtcct cctccatgga gatgtcggac ttcaccttca cgatgccctt
     4381 gtcctcgaag gtcatggtgc gctcccaaga gtagccctcg gggaaggact gcttgaagta
     4441 gttggggatg tcgtcggggt acttggtgaa ggccctgttg ccgtaggcga acgcggtggt
     4501 cagaatgtcg taggagaagg gcaggggggc tccctccttc acctccaggt tgatggtgtt
     4561 ggtgccgtcg tagggcttgc cctcgccctc gccctcgatc acgaaggcgt ggccattcac
     4621 gttgccctcc atcttcagct tgatcttcat gtcgggcttg attacgccca ttgtggtctc
     4681 ctcgcccttg ctcaccatcc gcgggagtct ctctcgggct gggacccaga tgtaggggcc
     4741 tgagaggtct tcaatgacac gtttcacttt gtgatagatc tcttcaaagc tgtcgccctc
     4801 tacgatggct gagaagcact ctgtgaactc ctgctccagc ttcgtggctc tgtcgaaggc
     4861 tttccgggct tgctcctctg tgatccgctt attgatctct agcacattct ccagggagcg
     4921 gggacggatg aagatggcga tggggtgcag gtgggccgcc tgcagccgcc gcacggcatt
     4981 ggccgagaca tcgaggatgc agtgcttccc ctgctctgct acctctcgca cagactggac
     5041 gctggtccca tagaggtggc tgttgtactg gccagcctca atgaacttgt gtgcctggat
     5101 gtccttctcc attttctccc gggaggagac aaagtggtaa tcctggccgt ctatctcata
     5161 ttcccgctta ggacgtgtcg tatgagggac acaggatcca aacttgtcgg ggaactcgga
     5221 gagaagatca tcgttggcac ggtctttggt gggtccaagg atgatgatgg gacgagcata
     5281 gtgcacttcc atctgggtca ccgtctcata gctcagaacc gagtcttctc gaccctgtga
     5341 tccagagctg gagccccagt ccttggcctt taaccttgac cactctcgtc gctcgacccg
     5401 ccgtttgctg ggaatgaagc caatgtcgtc ggtctcactg tcggagtgga cccgccgtgc
     5461 ttgccaccac tcttcgtcac caacgtcaat gacatgaagc acatccccga agcggaagct
     5521 cagggcctgg ctcaagaaac cgcagtcctt ggtcttgtcg taatcaaaca gggccctaat
     5581 gtagaagccc ctcttggggt tgcttcgcaa ggatgcagtc cctgagccta ggctactatt
     5641 catgagctgt tcccgaagat catggatctt ggcctcgaat cgactatact cttctggttt
     5701 atactgagcg atgatcgtga ccgtctgacc cgcattcttc agggcaatgg cagcctgttc
     5761 gtgactggca ttgcggaggt caacaccatt gaccgacagg atctggtccc ccttccgtag
     5821 ctccccactg aggtcggctg gacccccagc aaggatgaag gagatgaaga tgccttcacc
     5881 atcctcgccg cccacgatgt tgaagcccag gccggtggag ccccgatgga tcacgatccg
     5941 ccttggttcc cggggaatgt cttcctcccc cagcaggtcc ttggccacag gggagtagcg
     6001 ccgaggggaa gtgggggtca tggctgtggg gtagtcagtg cccaagtagc tgctatgact
     6061 gatctcattg tccaggtgct gagaatacga ggttgtgatg tctgggggag catagctgtc
     6121 actcaggtag gcattgctgg gcttggccac ctttaggtac acaacgtcat atgtgttctt
     6181 cagggctgcc acggcatcct cgtgcataac gtcctccagc cccacactgt tgaccgccag
     6241 gatcttgtct ccaatctgca acctgccatc cttgtgagca gcacctcctt cgatgatctt
     6301 cgttacatag atgctgttat ctccagggat gtgctggttc ccaacgcccc ccgcaatgct
     6361 gaagccaagt cctttaggcc ctttgatgag tttgatctcc atgacctttt cggctggggg
     6421 tttccggcgc atgacatagt ggcgaacgat ggaacctgcc tctttgaggg cctccaccgc
     6481 agctgaatgg gtcacctccc gaacatccac ttcatttaca aacaggatgc tgtcattgac
     6541 cctgaggcgg ccatcctggg ctgcagcccc accaggaatg atcttggtga taaaaatgga
     6601 cgggtcgtca ccgatgtgcg ggttgtcagt gccacctgcg atgctgaagc ccagacctga
     6661 gttacccctt tccaatgtga tctcctcata ctccatctcc ccctctgttc cattcacctg
     6721 caactcatat cctggggctt ctagggtgtc cgtgttgaca atcacagggg gagaattggc
     6781 ctggttgggg aggtgggccg ggctgtgttc cagagggggc gtgtcttcat cttggtagcg
     6841 gtatttcttg gcgcgcccca agtcctcttc agatatcagc ttttgctcgg cgcgccgggt
     6901 tgtcactata cagagacagt ccatggtggc ggctagcgaa gttcctatac tttctagaga
     6961 ataggaactt cwttgcctta acccagaaat tatcactgtt attctttaga atggtgcaaa
     7021 gagaagttcc tatacctttt gaagaatagg aacttcgaat tcgatatcaa gcttatcgat
     7081 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     7141 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     7201 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     7261 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     7321 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     7381 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     7441 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     7501 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     7561 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     7621 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt
     7681 cgacctcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     7741 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     7801 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7861 gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     7921 tggatctacc acacacaagg ctacttccct gattggcaga actacacacc agggccaggg
     7981 atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag
     8041 gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg
     8101 atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat
     8161 cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga
     8221 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8281 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8341 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc
     8401 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     8461 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     8521 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     8581 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     8641 ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     8701 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     8761 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     8821 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     8881 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     8941 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     9001 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     9061 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     9121 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     9181 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     9241 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     9301 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     9361 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     9421 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     9481 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg
     9541 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc
     9601 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg
     9661 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc
     9721 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc
     9781 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca
     9841 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca
     9901 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca
     9961 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga
    10021 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag
    10081 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
    10141 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac
    10201 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
    10261 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
    10321 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
    10381 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
    10441 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
    10501 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
    10561 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
    10621 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
    10681 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
    10741 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
    10801 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
    10861 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
    10921 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
    10981 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
    11041 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
    11101 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
    11161 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    11221 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    11281 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    11341 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    11401 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    11461 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    11521 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    11581 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    11641 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    11701 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    11761 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    11821 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    11881 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    11941 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    12001 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    12061 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    12121 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    12181 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    12241 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    12301 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    12361 gcacatttcc ccgaaaagtg ccacctgac
//
