LOCUS       sequence-258123-       10942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Synaptophysin-TdTomato and also tamoxifen dependant Cre 
            (OHT-Cre, aka Cre-ER).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10942)
  AUTHORS   Subramanian J, Michel K, Benoit M, Nedivi E
  TITLE     CPG15/Neuritin Mimics Experience in Selecting Excitatory Synapses 
            for Stabilization by Facilitating PSD95 Recruitment.
  JOURNAL   Cell Rep. 2019 Aug 6;28(6):1584-1595.e5. doi: 
            10.1016/j.celrep.2019.07.012.
  PUBMED    31390571
REFERENCE   2  (bases 1 to 10942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.07"; journalName: "Cell Rep"; date: 
            "2019-08-6- 6"; volume: "28"; issue: "6"; pages: "1584-1595.e5"
COMMENT     Sequence Label: pCAG-Synaptophysin-TdTomato-IRES2-OHT-Cre
FEATURES             Location/Qualifiers
     source          1..10942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        367..642
                     /label=chicken beta-actin promoter
     intron          643..1651
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1575..1597
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1659..1678
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1717..1726
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2653..4083
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    join(4095..4669,4670..4672,4673..4681)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4262..4279)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4489..4508
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             4685..5620
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5630..6652
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS
                     VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR
                     PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG
                     IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW
                     ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY
                     LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG
                     D"
     CDS             6665..7600
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             6713..7606
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     polyA_signal    7730..7851
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7767..7786)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7821..7840
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(7858..8313)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7995..8016)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8207..8226
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8340..8444
                     /gene="bla"
                     /label=AmpR promoter
     promoter        8446..8803
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8446..8637
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8469..8489)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8654..8789
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8838..9632
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             8838..9632
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8892..8911)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     9502..9521
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(9820..9839)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    9864..9911
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      10240..10828
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10729..10748
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catgggtcga ggtgagcccc acgttctgct tcactctccc catctccccc ccctccccac
      421 ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg gcgggggggg
      481 ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga
      541 ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg
      601 cggcggcggc ggccctataa aaagcgaagc gcgcggcggg cgggagtcgc tgcgttgcct
      661 tcgccccgtg ccccgctccg cgccgcctcg cgccgcccgc cccggctctg actgaccgcg
      721 ttactcccac aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg
      781 gtttaatgac ggctcgtttc ttttctgtgg ctgcgtgaaa gccttaaagg gctccgggag
      841 ggccctttgt gcggggggga gcggctcggg gggtgcgtgc gtgtgtgtgt gcgtggggag
      901 cgccgcgtgc ggcccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt
      961 tgtgcgctcc gcgtgtgcgc gaggggagcg cggccggggg cggtgccccg cggtgcgggg
     1021 gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg
     1081 tgtgggcgcg gcggtcgggc tgtaaccccc ccctgcaccc ccctccccga gttgctgagc
     1141 acggcccggc ttcgggtgcg gggctccgtg cggggcgtgg cgcggggctc gccgtgccgg
     1201 gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg gccggggagg
     1261 gctcggggga ggggcgcggc ggccccggag cgccggcggc tgtcgaggcg cggcgagccg
     1321 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat
     1381 ctggcggagc cgaaatctgg gaggcgccgc cgcaccccct ctagcgggcg cgggcgaagc
     1441 ggtgcggcgc cggcaggaag gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc
     1501 gtccccttct ccatctccag cctcggggct gccgcagggg gacggctgcc ttcggggggg
     1561 acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag cctctgctaa
     1621 ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg ttattgtgct
     1681 gtctcatcat tttggcaaag aattgccggg tgagccgcca ccatggacgt ggtgaatcag
     1741 ctggtggctg ggggtcagtt ccgggtggtc aaggagcccc ttggcttcgt gaaggtgctg
     1801 cagtgggtct ttgccatctt cgcctttgct acgtgcggca gctacaccgg agagcttcgg
     1861 ctgagcgtgg agtgtgccaa caagacggag agtgccctca acatcgaagt cgaatttgag
     1921 tacccattca ggctgcacca agtgtacttt gatgcaccct cctgcgttaa agggggcact
     1981 accaagatct tcctagttgg tgactactcc tcctcggctg aattctttgt caccgtggct
     2041 gtgtttgcct tcctctactc catgggggcc ctggccacct acatcttcct gcagaacaag
     2101 taccgagaga acaacaaagg gccaatgatg gacttcctgg ccacagcagt gttcgctttc
     2161 atgtggctag ttagctcatc cgcctgggcc aaaggcctgt ccgatgtgaa gatggccact
     2221 gacccagaga acattatcaa ggagatgcct atgtgccgcc agacaggaaa cacatgcaag
     2281 gaactgaggg accctgtgac ttcaggactc aacacctcgg tggtgtttgg cttcctgaac
     2341 ctggtgctct gggttggcaa cctatggttc gtgttcaagg agacaggctg ggccgcccca
     2401 ttcatgcgcg cacctccagg cgccccagaa aagcaaccag ctcctggcga tgcctacggc
     2461 gatgcgggct atgggcaggg ccccggaggc tatgggcccc aggactccta cgggcctcag
     2521 ggtggttatc aacccgatta cgggcagcca gccagcggcg gtggcggtgg ctacgggcct
     2581 cagggcgact atgggcagca aggctacggc caacagggtg cgcccacctc cttctccaat
     2641 cagatgtcgc gaatggtgag caagggcgag gaggtcatca aagagttcat gcgcttcaag
     2701 gtgcgcatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
     2761 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggcgg ccccctgccc
     2821 ttcgcctggg acatcctgtc cccccagttc atgtacggct ccaaggcgta cgtgaagcac
     2881 cccgccgaca tccccgatta caagaagctg tccttccccg agggcttcaa gtgggagcgc
     2941 gtgatgaact tcgaggacgg cggtctggtg accgtgaccc aggactcctc cctgcaggac
     3001 ggcacgctga tctacaaggt gaagatgcgc ggcaccaact tcccccccga cggccccgta
     3061 atgcagaaga agaccatggg ctgggaggcc tccaccgagc gcctgtaccc ccgcgacggc
     3121 gtgctgaagg gcgagatcca ccaggccctg aagctgaagg acggcggcca ctacctggtg
     3181 gagttcaaga ccatctacat ggccaagaag cccgtgcaac tgcccggcta ctactacgtg
     3241 gacaccaagc tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgag
     3301 cgctccgagg gccgccacca cctgttcctg gggcatggta ccggcagcac cggcagcggc
     3361 agctccggca ccgcctcctc cgaggacaac aacatggccg tcatcaaaga gttcatgcgc
     3421 ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt tcgagatcga gggcgagggc
     3481 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggcggcccc
     3541 ctgcccttcg cctgggacat cctgtccccc cagttcatgt acggctccaa ggcgtacgtg
     3601 aagcaccccg ccgacatccc cgattacaag aagctgtcct tccccgaggg cttcaagtgg
     3661 gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg tgacccagga ctcctccctg
     3721 caggacggca cgctgatcta caaggtgaag atgcgcggca ccaacttccc ccccgacggc
     3781 cccgtaatgc agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc
     3841 gacggcgtgc tgaagggcga gatccaccag gccctgaagc tgaaggacgg cggccactac
     3901 ctggtggagt tcaagaccat ctacatggcc aagaagcccg tgcaactgcc cggctactac
     3961 tacgtggaca ccaagctgga catcacctcc cacaacgagg actacaccat cgtggaacag
     4021 tacgagcgct ccgagggccg ccaccacctg ttcctgtacg gcatggacga gctgtacaag
     4081 tagcccggga tccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg
     4141 gaataaggcc ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc
     4201 aatgtgaggg cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc
     4261 cctctcgcca aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa
     4321 gcttcttgaa gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct
     4381 ggcgacaggt gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca
     4441 caaccccagt gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca
     4501 agcgtattca acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat
     4561 ctggggcctc ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc
     4621 cccccgaacc acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacaac
     4681 catggctgga gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc
     4741 taagaagaac agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga
     4801 tgctgagccc cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc
     4861 gatgatgggc ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc
     4921 gaagagggtg ccaggctttg tggatttgac cctccatgat caggtccacc ttctagaatg
     4981 tgcctggcta gagatcctga tgattggtct cgtctggcgc tccatggagc acccagtgaa
     5041 gctactgttt gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat
     5101 ggtggagatc ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca
     5161 gggagaggag tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt
     5221 tctgtccagc accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa
     5281 gatcacagac actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca
     5341 ccagcggctg gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg
     5401 catggagcat ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct
     5461 ggaggcggcg gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga
     5521 ggagacggac caaagccact tggccactgc gggctctact tcatcgcatt ccttgcaaaa
     5581 gtattacatc acgggggagg cagagggttt ccctgccaca gctgtcgaca atttactgac
     5641 cgtacaccaa aatttgcctg cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa
     5701 cctgatggac atgttcaggg atcgccaggc gttttctgag catacctgga aaatgcttct
     5761 gtccgtttgc cggtcgtggg cggcatggtg caagttgaat aaccggaaat ggtttcccgc
     5821 agaacctgaa gatgttcgcg attatcttct atatcttcag gcgcgcggtc tggcagtaaa
     5881 aactatccag caacatttgg gccagctaaa catgcttcat cgtcggtccg ggctgccacg
     5941 accaagtgac agcaatgctg tttcactggt tatgcggcgg atccgaaaag aaaacgttga
     6001 tgccggtgaa cgtgcaaaac aggctctagc gttcgaacgc actgatttcg accaggttcg
     6061 ttcactcatg gaaaatagcg atcgctgcca ggatatacgt aatctggcat ttctggggat
     6121 tgcttataac accctgttac gtatagccga aattgccagg atcagggtta aagatatctc
     6181 acgtactgac ggtgggagaa tgttaatcca tattggcaga acgaaaacgc tggttagcac
     6241 cgcaggtgta gagaaggcac ttagcctggg ggtaactaaa ctggtcgagc gatggatttc
     6301 cgtctctggt gtagctgatg atccgaataa ctacctgttt tgccgggtca gaaaaaatgg
     6361 tgttgccgcg ccatctgcca ccagccagct atcaactcgc gccctggaag ggatttttga
     6421 agcaactcat cgattgattt acggcgctaa ggatgactct ggtcagagat acctggcctg
     6481 gtctggacac agtgcccgtg tcggagccgc gcgagatatg gcccgcgctg gagtttcaat
     6541 accggagatc atgcaagctg gtggctggac caatgtaaat attgtcatga actatatccg
     6601 taacctggat agtgaaacag gggcaatggt gcgcctgctg gaagatggcg atctcgagcc
     6661 atctgctgga gacatgagag ctgccaacct ttggccaagc ccgctcatga tcaaacgctc
     6721 taagaagaac agcctggcct tgtccctgac ggccgaccag atggtcagtg ccttgttgga
     6781 tgctgagccc cccatactct attccgagta tgatcctacc agacccttca gtgaagcttc
     6841 gatgatgggc ttactgacca acctggcaga cagggagctg gttcacatga tcaactgggc
     6901 gaagagggtg ccaggctttg tggatttgac cctccatgat caggtccacc ttctagaatg
     6961 tgcctggcta gagatcctga tgattggtct cgtctggcgc tccatggagc acccagtgaa
     7021 gctactgttt gctcctaact tgctcttgga caggaaccag ggaaaatgtg tagagggcat
     7081 ggtggagatc ttcgacatgc tgctggctac atcatctcgg ttccgcatga tgaatctgca
     7141 gggagaggag tttgtgtgcc tcaaatctat tattttgctt aattctggag tgtacacatt
     7201 tctgtccagc accctgaagt ctctggaaga gaaggaccat atccaccgag tcctggacaa
     7261 gatcacagac actttgatcc acctgatggc caaggcaggc ctgaccctgc agcagcagca
     7321 ccagcggctg gcccagctcc tcctcatcct ctcccacatc aggcacatga gtaacaaagg
     7381 catggagcat ctgtacagca tgaagtgcaa gaacgtggtg cccctctatg acctgctgct
     7441 ggaggcggcg gacgcccacc gcctacatgc gcccactagc cgtggagggg catccgtgga
     7501 ggagacggac caaagccact tggccactgc gggctctact tcatcgcatt ccttgcaaaa
     7561 gtattacatc acgggggagg cagagggttt ccctgccaca gcttgatagc ggccgcgact
     7621 ctagatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
     7681 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
     7741 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     7801 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt aaggcgtaaa
     7861 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     7921 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     7981 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     8041 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     8101 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     8161 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     8221 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     8281 ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg ggaaatgtgc
     8341 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     8401 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag gcggaaagaa
     8461 ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     8521 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     8581 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     8641 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     8701 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     8761 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat caagagacag
     8821 gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct ccggccgctt
     8881 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     8941 ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
     9001 gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc acgacgggcg
     9061 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
     9121 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
     9181 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
     9241 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
     9301 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
     9361 aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga
     9421 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
     9481 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
     9541 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
     9601 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg aaatgaccga
     9661 ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct tctatgaaag
     9721 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
     9781 catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga aggagacaat
     9841 accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc acggtgttgg
     9901 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     9961 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
    10021 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg ccatagcctc
    10081 aggttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    10141 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    10201 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    10261 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    10321 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    10381 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    10441 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    10501 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    10561 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    10621 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    10681 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    10741 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    10801 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    10861 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    10921 taaccgtatt accgccatgc at
//
