LOCUS       sequence-258125-       11995 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses cpg15 and TdTomato upon coexpression with Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11995)
  AUTHORS   Subramanian J, Michel K, Benoit M, Nedivi E
  TITLE     CPG15/Neuritin Mimics Experience in Selecting Excitatory Synapses 
            for Stabilization by Facilitating PSD95 Recruitment.
  JOURNAL   Cell Rep. 2019 Aug 6;28(6):1584-1595.e5. doi: 
            10.1016/j.celrep.2019.07.012.
  PUBMED    31390571
REFERENCE   2  (bases 1 to 11995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2019.07"; journalName: "Cell Rep"; date: 
            "2019-08-6- 6"; volume: "28"; issue: "6"; pages: "1584-1595.e5"
COMMENT     Sequence Label: pFudio-Cpg15-IRES2-TdTomatoW
FEATURES             Location/Qualifiers
     source          1..11995
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2611..3010
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     3658..3677
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(3899..3932)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(3983..4016)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(4025..5455)
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVCMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGRYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     misc_feature    join(5456..5464,5465..5467,5468..5470,5471..6045)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5632..5651)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     5861..5878
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(6413..6436)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     regulatory      6526..6535
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    6545..6578
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    6629..6662
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    6687..7275
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6740..6760)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7158..7169)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7278..7294)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7279..7295)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             7800..7980
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8006..8023)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8012..8236
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8282..8710
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8369..8388)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8579..8600
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8719..8739)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8724..9053
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8904..9039
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8966..8985
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9101..9148
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9167..9541
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9671..9792
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9708..9727)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9762..9781
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9841..9857)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9841..9857)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9854..9876)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9865..9881
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9889..9895,9896..9913,9914..9919))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9934..9955
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10072..10089)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10243..10831)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10323..10342)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11002..11793,11794..11862))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11625..11644
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11863..11967)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agggtgcagc ggcctccgcg ccgggttttg gcgcctcccg
     2641 cgggcgcccc cctcctcacg gcgagcgctg ccacgtcaga cgaagggcgc aggagcgttc
     2701 ctgatccttc cgcccggacg ctcaggacag cggcccgctg ctcataagac tcggccttag
     2761 aaccccagta tcagcagaag gacattttag gacgggactt gggtgactct agggcactgg
     2821 ttttctttcc agagagcgga acaggcgagg aaaagtagtc ccttctcggc gattctgcgg
     2881 agggatctcc gtggggcggt gaacgccgat gattatataa ggacgcgccg ggtgtggcac
     2941 agctagttcc gtcgcagccg ggatttgggt cgcggttctt gtttgtggat cgctgtgatc
     3001 gtcacttggt gagttgcggg ctgctgggct ggccggggct ttcgtggccg ccgggccgct
     3061 cggtgggacg gaagcgtgtg gagagaccgc caagggctgt agtctgggtc cgcgagcaag
     3121 gttgccctga actgggggtt ggggggagcg cacaaaatgg cggctgttcc cgagtcttga
     3181 atggaagacg cttgtaaggc gggctgtgag gtcgttgaaa caaggtgggg ggcatggtgg
     3241 gcggcaagaa cccaaggtct tgaggccttc gctaatgcgg gaaagctctt attcgggtga
     3301 gatgggctgg ggcaccatct ggggaccctg acgtgaagtt tgtcactgac tggagaactc
     3361 gggtttgtcg tctggttgcg ggggcggcag ttatgcggtg ccgttgggca gtgcacccgt
     3421 acctttggga gcgcgcgcct cgtcgtgtcg tgacgtcacc cgttctgttg gcttataatg
     3481 cagggtgggg ccacctgccg gtaggtgtgc ggtaggcttt tctccgtcgc aggacgcagg
     3541 gttcgggcct agggtaggct ctcctgaatc gacaggcgcc ggacctctgg tgaggggagg
     3601 gataagtgag gcgtcagttt ctttggtcgg ttttatgtac ctatcttctt aagtagctga
     3661 agctccggtt ttgaactatg cgctcggggt tggcgagtgt gttttgtgaa gttttttagg
     3721 caccttttga aatgtaatca tttgggtcaa tatgtaattt tcagtgttag actagtaaag
     3781 cttctgcagg tcgactctag aaaattgtcc gctaaattct ggccgttttt ggcttttttg
     3841 ttagacgaag cttgggctgc aggtcgactc tagaggatcc tctagagtcg actccggaat
     3901 aacttcgtat aggatacttt atacgaagtt atgcagaatg gtagctggat tgtagctgct
     3961 attagcaata tgaaacctct taataacttc gtatagcata cattatacga agttatggcg
     4021 cgccttactt gtacagctcg tccatgccgt acaggaacag gtggtggcgg ccctcggagc
     4081 gctcgtactg ttccacgatg gtgtagtcct cgttgtggga ggtgatgtcc agcttggtgt
     4141 ccacgtagta gtagccgggc agttgcacgg gcttcttggc catgtagatg gtcttgaact
     4201 ccaccaggta gcggccgccg tccttcagct tcagggcctg gtggatctcg cccttcagca
     4261 cgccgtcgcg ggggtacagg cgctcggtgg aggcctccca gcccatggtc ttcttctgca
     4321 ttacggggcc gtcggggggg aagttggtgc cgcgcatctt caccttgtag atcagcgtgc
     4381 cgtcctgcag ggaggagtcc tgggtcacgg tcaccagacc gccgtcctcg aagttcatca
     4441 cgcgctccca cttgaagccc tcggggaagg acagcttctt gtaatcgggg atgtcggcgg
     4501 ggtgcttcac gtacgccttg gagccgtaca tgaactgggg ggacaggatg tcccaggcga
     4561 agggcagggg gccgcccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc
     4621 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt catggagccc tccatgcgca
     4681 ccttgaagcg catgaactct ttgatgacgg ccatgttgtt gtcctcggag gaggcggtgc
     4741 cggagctgcc gctgccggtg ctgccggtgc catgccccag gaacaggtgg tggcggccct
     4801 cggagcgctc gtactgttcc acgatggtgt agtcctcgtt gtgggaggtg atgtccagct
     4861 tggtgtccac gtagtagtag ccgggcagtt gcacgggctt cttggccatg tagatggtct
     4921 tgaactccac caggtagtgg ccgccgtcct tcagcttcag ggcctggtgg atctcgccct
     4981 tcagcacgcc gtcgcggggg tacaggcgct cggtggaggc ctcccagccc atggtcttct
     5041 tctgcattac ggggccgtcg ggggggaagt tggtgccgcg catcttcacc ttgtagatca
     5101 gcgtgccgtc ctgcagggag gagtcctggg tcacggtcac cagaccgccg tcctcgaagt
     5161 tcatcacgcg ctcccacttg aagccctcgg ggaaggacag cttcttgtaa tcggggatgt
     5221 cggcggggtg cttcacgtac gccttggagc cgtacatgaa ctggggggac aggatgtccc
     5281 aggcgaaggg cagggggccg cccttggtca ccttcagctt ggcggtctgg gtgccctcgt
     5341 aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcatg gagccctcca
     5401 tgcacacctt gaagcgcatg aactctttga tgacctcctc gcccttgctc accatggttg
     5461 tggcaccggt attatcatcg tgtttttcaa aggaaaacca cgtccccgtg gttcgggggg
     5521 cctagacgtt tttttaacct cgactaaaca catgtaaagc atgtgtaccg aggccccaga
     5581 tcagatccca tacaatgggg taccttctgg gcatccttca gccccttgtt gaatacgctt
     5641 gaggagagcc atttgactct ttccacaact atccaactca caacgtggca ctggggttgt
     5701 gccgcctttg caggtgtatc ttatacacgt ggcttttggc cgcagaggca cctgtcgcca
     5761 ggtggggggt tccgctgcct gcaaagggtc gctacagacg ttgtttgtct tcaagaagct
     5821 tccagaggaa ctgcttcctt cacgacattc aacagacctt gcattccttt ggcgagaggg
     5881 gaaagacccc taggaatgct cgtcaagaag acagggccag gtttccgggc cctcacattg
     5941 ccaaaagacg gcaatatggt ggaaaataac atatagacaa acgcacaccg gccttattcc
     6001 aagcggcttc ggccagtaac gttagggggg ggggagggag aggggcggat cccgggcccg
     6061 cggtaccgtc gactcagaag gaaagccagg tcgctaaagc tgccgagaga gacaccagga
     6121 gcacggaaag cgccgggagc agggaccccg ccgccccgtt gccgctgccg cagagttcga
     6181 ataagctgcc ttggatattg aggtttttcg attcttttct cagtttatcc cacatatctt
     6241 tcgccccttc ctggcaatcc gtaagagctg tgaccgtgca gctgtggaaa tcctcccagt
     6301 atgtgcacac ggtcttgatg ttcgtcttgt cgtccaggcc ctgcgggtag ttggccatgc
     6361 tgtcacccag cttgagcaaa cagtctgaaa agcccttaaa gactgcatcg cacttgtcat
     6421 catcgtcctt atagtcggta ccgcctgctg ctctcacggc ctgcaccagg taagctattt
     6481 gcaccgcgag gatcagtgaa atatatctgc cgttcaactt aagtcccatg gtggcggcgc
     6541 tagcataact tcgtataaag tatcctatac gaagttattt gccttaaccc agaaattatc
     6601 actgttattc tttagaatgg tgcaaagaat aacttcgtat aatgtatgct atacgaagtt
     6661 atgaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat
     6721 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     6781 ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct
     6841 ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca
     6901 ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt
     6961 ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg
     7021 cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga
     7081 aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt
     7141 ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     7201 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     7261 gggccgcctc cccgcatcga taccgtcgac ctcgagacct agaaaaacat ggagcaatca
     7321 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg
     7381 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag
     7441 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     7501 aacgaagaca agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt
     7561 ggcagaacta cacaccaggg ccagggatca gatatccact gacctttgga tggtgctaca
     7621 agctagtacc agttgagcaa gagaaggtag aagaagccaa tgaaggagag aacacccgct
     7681 tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagta ttagagtgga
     7741 ggtttgacag ccgcctagca tttcatcaca tggcccgaga gctgcatccg gactgtactg
     7801 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7861 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7921 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7981 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     8041 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     8101 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     8161 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     8221 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
     8281 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     8341 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     8401 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8461 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     8521 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8581 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     8641 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8701 aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     8761 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     8821 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     8881 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     8941 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tctgcctctg
     9001 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc
     9061 cgggagcttg tatatccatt ttcggatctg atcagcacgt gttgacaatt aatcatcggc
     9121 atagtatatc ggcatagtat aatacgacaa ggtgaggaac taaaccatgg ccaagttgac
     9181 cagtgccgtt ccggtgctca ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga
     9241 ccggctcggg ttctcccggg acttcgtgga ggacgacttc gccggtgtgg tccgggacga
     9301 cgtgaccctg ttcatcagcg cggtccagga ccaggtggtg ccggacaaca ccctggcctg
     9361 ggtgtgggtg cgcggcctgg acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa
     9421 cttccgggac gcctccgggc cggccatgac cgagatcggc gagcagccgt gggggcggga
     9481 gttcgccctg cgcgacccgg ccggcaactg cgtgcacttc gtggccgagg agcaggactg
     9541 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
     9601 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
     9661 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     9721 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     9781 caatgtatct tatcatgtct gtataccgtc gacctctagc tagagcttgg cgtaatcatg
     9841 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     9901 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     9961 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
    10021 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
    10081 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
    10141 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
    10201 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
    10261 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
    10321 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
    10381 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
    10441 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
    10501 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
    10561 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
    10621 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
    10681 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
    10741 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
    10801 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
    10861 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
    10921 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10981 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    11041 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    11101 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    11161 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    11221 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    11281 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    11341 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    11401 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11461 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    11521 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    11581 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    11641 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11701 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11761 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11821 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11881 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
    11941 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgac
//
