LOCUS       sequence-258126-        6113 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Targeting expression to neuronal and glial cell types in mice, 
            non-human primates, and human retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6113)
  AUTHORS   Juttner J, Szabo A, Gross-Scherf B, Morikawa RK, Rompani SB, Hantz 
            P, Szikra T, Esposti F, Cowan CS, Bharioke A, Patino-Alvarez CP, 
            Keles O, Kusnyerik A, Azoulay T, Hartl D, Krebs AR, Schubeler D, 
            Hajdu RI, Lukats A, Nemeth J, Nagy ZZ, Wu KC, Wu RH, Xiang L, Fang 
            XL, Jin ZB, Goldblum D, Hasler PW, Scholl HPN, Krol J, Roska B
  TITLE     Targeting neuronal and glial cell types with synthetic promoter AAVs
            in mice, non-human primates and humans.
  JOURNAL   Nat Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2.
  PUBMED    31285614
REFERENCE   2  (bases 1 to 6113)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2019 Jul 8. pii: 10.1038/s41593-019-0431-2. doi: 
            10.1038/s41593-019-0431-2."
COMMENT     Sequence Label: VIII_pAAV-ProB9-CatCh-GFP-WPRE
FEATURES             Location/Qualifiers
     source          1..6113
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     regulatory      557..566
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             563..1489
                     /codon_start=1
                     /product="N-terminal fragment of channelrhodopsin-2 from 
                     the alga Chlamydomonas reinhardtii (Nagel et al., 2003)"
                     /label=ChR2
                     /note="blue light-activated cation channel"
                     /translation="MDYGGALSAVGRELLFVTNPVVVNGSVLVPEDQCYCAGWIESRGT
                     NGAQTASNVLQWLAAGFSILLLMFYAYQTWKSTCGWEEIYVCAIEMVKVILEFFFEFKN
                     PSMLYLATGHRVQWLRYAEWLLTCPVICIHLSNLTGLSNDYSRRTMGLLVSDIGTIVWG
                     ATSAMATGYVKVIFFCLGLCYGANTFFHAAKAYIEGYHTVPKGRCRQVVTGMAWLFFVS
                     WGMFPILFILGPEGFGVLSVYGSTVGHTIIDLMSKNCWGLLGHYLRVLIHEHILIHGDI
                     RKTTKLNIGGTEIEVETLVEDEAEAGAV"
     CDS             join(1493..1495,1496..1498,1499..2212)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1493..1495,1496..1498,1499..2209)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1538..1559)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1799..1818)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2146..2167
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2240..2828
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2293..2313)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2711..2722)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2860..3336
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3059..3078)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3376..3516
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3376..3505
                     /label=AAV2 ITR
     rep_origin      3591..4046
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3678..3697)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3888..3909
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4063..4082)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4182..4204
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4242..4260)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4328..4432
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4433..4501,4502..5293)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4651..4670)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5464..6052
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5953..5972
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtatctgc ttaaaagctc aggaagccgg tgctgacatt
      181 cttgcttggg ggcccagaat ttaaacttag tcactgccca aggcccttgt tttaggaaag
      241 aatgagattg ttgttgcata accagctgcg agaacagatg gaggcagcgc ggctcacctc
      301 gggggctccg tgcccgctgt gcgccgaagt ccaggctgag gaggaggtca aatttcatta
      361 ccaaagaggc cgagttctgg ggacgctaag cccccacaca aaaaatccct ggaggctcat
      421 caccagctga ggcagggcca tcagagaatt tgatgggtag ccaggcgtgc taacgccagc
      481 cctttgtcgg gtctttgttc cacccctggc tgcactggta cagaaccact tagctgtgat
      541 taaaaggagg ggatccgcca ccatggatta tggaggcgcc ctgagtgccg ttgggcgcga
      601 gctgctattt gtaacgaacc cagtagtcgt caatggctct gtacttgtgc ctgaggacca
      661 gtgttactgc gcgggctgga ttgagtcgcg tggcacaaac ggtgcccaaa cggcgtcgaa
      721 cgtgctgcaa tggcttgctg ctggcttctc catcctactg cttatgtttt acgcctacca
      781 aacatggaag tcaacctgcg gctgggagga gatctatgtg tgcgctatcg agatggtcaa
      841 ggtgattctc gagttcttct tcgagtttaa gaacccgtcc atgctgtatc tagccacagg
      901 ccaccgcgtc cagtggttgc gttacgccga gtggcttctc acctgcccgg tcatttgcat
      961 tcacctgtca aacctgacgg gcttgtccaa cgactacagc aggcgcacca tgggtctgct
     1021 tgtgtctgat attggcacaa ttgtgtgggg cgccacttcc gccatggcca ccggatacgt
     1081 caaggtcatc ttcttctgcc tgggtctgtg ttatggtgct aacacgttct ttcacgctgc
     1141 caaggcctac atcgagggtt accacaccgt gccgaagggc cggtgtcgcc aggtggtgac
     1201 tggcatggct tggctcttct tcgtatcatg gggtatgttc cccatcctgt tcatcctcgg
     1261 ccccgagggc ttcggcgtcc tgagcgtgta cggctccacc gtcggccaca ccatcattga
     1321 cctgatgtcg aagaactgct ggggtctgct cggccactac ctgcgcgtgc tgatccacga
     1381 gcatatcctc atccacggcg acattcgcaa gaccaccaaa ttgaacattg gtggcactga
     1441 gattgaggtc gagacgctgg tggaggacga ggccgaggct ggcgcggtac ccatggtgag
     1501 caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt
     1561 aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct
     1621 gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac
     1681 caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga
     1741 cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga
     1801 cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg
     1861 catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga
     1921 gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa
     1981 ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta
     2041 ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag
     2101 cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga
     2161 gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagt gataagaatt
     2221 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2281 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     2341 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     2401 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     2461 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     2521 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     2581 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     2641 gtcctttcct tggctgctcg cctatgttgc cacctggatt ctgcgcggga cgtccttctg
     2701 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     2761 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     2821 ctccccgcat cgataccgag cgctgctcga gagatctacg ggtggcatcc ctgtgacccc
     2881 tccccagtgc ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta
     2941 ataaaattaa gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg
     3001 aggggggtgg tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc
     3061 tattgggaac caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc
     3121 tgggttcaag cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg
     3181 accaggctca gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg
     3241 ctggtctcca actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga
     3301 ttacaggcgt gaaccactgc tcccttccct gtccttctga ttttgtaggt aaccacgtgc
     3361 ggaccgagcg gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     3421 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     3481 cctcagtgag cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc
     3541 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     3601 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     3661 cagcgcctta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     3721 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     3781 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     3841 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     3901 ccaaactgga acaacactca actctatctc gggctattct tttgatttat aagggatttt
     3961 gccgatttcg gtctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     4021 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     4081 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     4141 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     4201 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     4261 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     4321 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4381 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4441 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4501 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4561 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4621 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4681 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4741 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4801 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4861 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4921 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4981 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5041 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5101 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5161 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5221 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5281 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     5341 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     5401 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     5461 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     5521 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     5581 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     5641 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     5701 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     5761 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     5821 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     5881 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5941 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     6001 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     6061 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
