LOCUS       sequence-258130-        8817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene targeting in marmoset cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8817)
  AUTHORS   Yoshimatsu S, Okahara J, Sone T, Takeda Y, Nakamura M, Sasaki E, 
            Kishi N, Shiozawa S, Okano H
  TITLE     Robust and efficient knock-in in embryonic stem cells and 
            early-stage embryos of the common marmoset using the CRISPR-Cas9 
            system.
  JOURNAL   Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1038/s41598-018-37990-w.
  PUBMED    30728412
REFERENCE   2  (bases 1 to 8817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-02-6"; volume: "9(1):1528. doi"; pages: " 
            10.1038/s41598-018-37990-w"
COMMENT     Sequence Label: pKI-cjPLP1e2-A39T
FEATURES             Location/Qualifiers
     source          1..8817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1..23
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15..32
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     16..32
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    73..93
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    2094..2127
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    2127..2151
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        2158..2657
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(2193..2216)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2590..2609
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2681..3277
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2681..2700)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3177..3197
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             3284..4279
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     protein_bind    complement(4645..4669)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    4669..4702
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(6152..6172)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(6233..6249)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6233..6249)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6246..6268)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6257..6273
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6281..6287,6288..6305,6306..6311))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6326..6347
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6464..6481)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6635..7223)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6715..6734)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7394..8185,8186..8254))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8017..8036
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8255..8359)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8427..8445
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8483..8505)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8605..8624
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 cccagtcacg acgttgtaaa acgacggcca gtgccaagct tgcatgcctg caggtcggga
       61 tatctgaatt atcaactttg tataataaag ttgaaggtgc actccatatg acatcttaga
      121 gcatgggact agtacttgga acacagctgc tttcagagtc tgtgacttct tgtgtgcctt
      181 tcctgtttct cagcaacact ggcatggggc ctgggatacc aggtctgggg atctcaggga
      241 ctcttagcac tttaagacac atgtgttccc aggccctggt gtgttctcct agtgccagaa
      301 agatgtttca tgctttgctg actttgtata aagtctgttt gtagctgttt tgacagaatc
      361 tcagtgtaga actgaaggtg ggcacgttag ccaagctgca ctataggagg acaaaaccac
      421 catgcaaagt gtccaaaaat tattaagccc atgtttttat tatagtgagt aaagcagtct
      481 tcccattttc atttcttgtc ctatgctagc tccctcctgt ttggactgct cctctcatat
      541 gtaaactaag aagaatcaag cattctttgc aacaaataca cacgatgctc aaaaatatcc
      601 aggagcatgc aatttccaaa gtttccttca cctggaatgt tcttcaggct aaaatcctgt
      661 ctgacaatac cagcatctct ggcctgcact catcccttcc tggacctgca agtgcatcta
      721 ccctctgtta ccacttactt ggctgcctaa attgttagtt taaaatatta ggtctatcta
      781 gctaaatctt cctcaggaat ctaaagactc ccataaggca gagtctgtgt cttttctctc
      841 ttcatatccc atataacacc cactataatg ctgggcatat agtgagtatt tcataaatag
      901 ttgatgaatg actaaaataa gcaagcaaac aaacagacca gagcaataag gaaggaggga
      961 ctgatttcat gatctctctg gcttgctatt tgaattgctg aattattatt gtttgttaaa
     1021 tatatttttt agaaattctg ggaattaaag gtaaggattt tttttttctt tccttttttt
     1081 tttttttctt gagacagaat cttgctcttg tcacccaggc tggagtgcaa tggcgcaatc
     1141 ttggctcact gcaacctcca cttcctgggt tcaagagatt ttcctgcctc agccccctga
     1201 gtagctgaga ttacaggcat gtgccaccat gcctggctaa ttttcatatc tttagtagag
     1261 atggtgtttc accatgttgg ccaagctggt ctcgaactcc tgacctcagg taatcctcct
     1321 tagcctccca aagtgctggg attacaggtg tgagccacgg cgccaggcca aggatttatt
     1381 ttcaatgtaa ggattctggg tcgatctcac atgctgaaag ccaaaaccct tagtttctcc
     1441 tgcttcttga ctctggggtg cccactatct ccaagcctgt aagtgcagag cctggctggc
     1501 ctggaagaga gctttgagtg gcatgagcta cctactggat gtgcctgact gtttcccctt
     1561 cttcttcccc aggcttgtta gaatgttgcg ctcgttgctt agttggagcc ccctttgctt
     1621 ccctggtggc cactggattg tgtttctttg gggtggcact gttctgtggc tgtggacatg
     1681 aaaccctcac tggcacagaa aagctaattg aaacctattt ctccaaaaac tatcaagact
     1741 atgagtatct catcaatgtg taagtacttg ccctcccaca cagacccatc tttttttttt
     1801 ccctctctcc atcctggaga tagagtactc taatatctta gtaactaggg gatgggtgga
     1861 gccagacagg attcctgagt cttccctctg tgcagatttc tcctgttcca cattcaaatc
     1921 ctcaaatgtg gggaaattgc cagattcaga aaggagaggt ctgcctgcct gcctgcctgc
     1981 cagcctttcc cagcccctgt tttctcacac gtgctctgac ttctgctagg tgtggttaat
     2041 attgcccaag ttggagcctc cacagtggta ggtatggaga acccaagaga ggcataactt
     2101 cgtataatgt atgctatacg aagttatcaa gtttgtacaa aaaagcagaa ttctaccggg
     2161 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
     2221 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
     2281 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
     2341 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
     2401 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
     2461 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
     2521 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
     2581 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
     2641 catctccggg cctttcgacc gatcatcaag cttgatcctc atgaccgagt acaagcccac
     2701 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     2761 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     2821 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     2881 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     2941 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     3001 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     3061 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     3121 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     3181 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     3241 accgcgcacc tggtgcatga cccgcaagcc cggtgccgga tccatgccca cgctactgcg
     3301 ggtttatata gacggtcccc acgggatggg gaaaaccacc accacgcaac tgctggtggc
     3361 cctgggttcg cgcgacgata tcgtctacgt acccgagccg atgacttact ggcgggtgct
     3421 gggggcttcc gagacaatcg cgaacatcta caccacacaa caccgcctcg accagggtga
     3481 gatatcggcc ggggacgcgg cggtggtaat gacaagcgcc cagataacaa tgggcatgcc
     3541 ttatgccgtg accgacgccg ttctggctcc tcatatcggg ggggaggctg ggagctcaca
     3601 tgccccgccc ccggccctca ccctcatctt cgaccgccat cccatcgccg ccctcctgtg
     3661 ctacccggcc gcgcggtacc ttatgggcag catgaccccc caggccgtgc tggcgttcgt
     3721 ggccctcatc ccgccgacct tgcccggcac caacatcgtg cttggggccc ttccggagga
     3781 cagacacatc gaccgcctgg ccaaacgcca gcgccccggc gagcggctgg acctggctat
     3841 gctggctgcg attcgccgcg tttacgggct acttgccaat acggtgcggt atctgcagtg
     3901 cggcgggtcg tggcgggagg actggggaca gctttcgggg acggccgtgc cgccccaggg
     3961 tgccgagccc cagagcaacg cgggcccacg accccatatc ggggacacgt tatttaccct
     4021 gtttcgggcc cccgagttgc tggcccccaa cggcgacctg tataacgtgt ttgcctgggc
     4081 cttggacgtc ttggccaaac gcctccgttc catgcacgtc tttatcctgg attacgacca
     4141 atcgcccgcc ggctgccggg acgccctgct gcaacttacc tccgggatgg tccagaccca
     4201 cgtcaccacc cccggctcca taccgacgat atgcgacctg gcgcgcacgt ttgcccggga
     4261 gatgggggag gctaactgag ctctagctta tcgactcgcc gatagtggaa accgacgccc
     4321 cagcactcgt ccgagggcaa aggaatagtc gagaaattga tgatctatta aacaataaag
     4381 atgtccacat ggaagttttt cctgtcatac tttgttaaga agggtgagaa cagagtacct
     4441 acattttgaa tggaaggatt ggagctacgg gggtgggggt ggggtgggat tagataaatg
     4501 cctgctcttt actgaaggct ctttactatt gctttatgat aatgtttcat agttggatat
     4561 cataatttaa acaagcaaaa ccaaattaag ggccagctca ttcctcccac tcatgatcta
     4621 tagatcctct agagtcgagg gctgcagcag ctttcttgta caaagtggat aacttcgtat
     4681 aatgtatgct atacgaagtt attctaagcc tctcctgttc ctagaacaac ttaggctcct
     4741 gttccttcac ccacctttct tcttacctag ctccccactc ctgtgaggtg gcatgtctga
     4801 aggatcgggc agggagagta gggccctggt tctccaggtc acagggtaag caagatgtgg
     4861 cagaaagatc atatgtttct ggtcacatac actgtgtctt cacttatttc aacaaagata
     4921 gggtaaaagt atgcaggagg aacctggtga ttcctctaga gaatccctag ctttgttagg
     4981 gtgcctgctc tggcgtatac cacatttatg tgagtaaagg agctgggtct tcaattaata
     5041 cgattccttc gtctctttca tctccctgtt actgcaggat ccatgccttc cagtatgtca
     5101 tctatggaac tgcctctttc ttcttccttt atggggccct cctgctggct gagggcttct
     5161 acaccaccgg cgcagtcagg cagatctttg gcgactacaa gaccaccatc tgcggcaagg
     5221 gcctgagcgc aacggtaaca gggggccaga aggggagggg ttccagaggc caacatcaag
     5281 ctcattcttt ggagcgggtg tgtcattgtt tgggaaaatg gctaggacat cccgacaagg
     5341 tgatcatcct caggattttg tggcaataac aaggggtggg ggaaaattgg gcgtgagtct
     5401 gtggcctcgt ccccacccaa ggctgggtcc tctctagggg cccggcattt gagtgaggaa
     5461 gcgatggctg cagccgaacg agaagatcag gaagaacgtg gtgcccagct ggcttagcct
     5521 cacctttcaa aggttcccta agcaaatttc ttttcaaaat ggaaagcatg agttatgtag
     5581 gatgctttgt acaatcagac catttctaag ccacctgttg gtatcccttt actcccttcc
     5641 tagcagggac cacaagagtg gatataactg gacaagagtc taaaatactg ctcgtgagat
     5701 tgagacttgg acacctgatc tgagaaggag gattgataat aaaaattaaa taattgactc
     5761 caaggtgaaa tttaccatat tccgggatcc tactctgttg gaagtcccag agggaattgg
     5821 agtagatctg catgttgaat gttttatcat tgtctgaggg ttatccatct gtgctttgag
     5881 tggggtgcac attggttttc agtatctgga gttaaagatc ctttgagggc tgaacatgtg
     5941 caggttctag ccatgtcttt ttgtggccag cagtcagctt agaaaggttt tgtatatgaa
     6001 attttaatct cctattggct ttgttcaatg actagggaac aaaaatgttc ccgtggcaag
     6061 gaacattttc taagctcagc ctaaggcaca aaacggcacg actttctttc aagatctatt
     6121 ttgatttctt tacaccttat ctgcccagaa acaacttttc tatacaaagt tgatagcttg
     6181 atatcaatac tctagaggat ccccgggtac cgagctcgaa ttcgtaatca tggtcatagc
     6241 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6301 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6361 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6421 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6481 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6541 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6601 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6661 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6721 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6781 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6841 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6901 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6961 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     7021 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     7081 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     7141 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     7201 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7261 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7321 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7381 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7441 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7501 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7561 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7621 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7681 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7741 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7801 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7861 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7921 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7981 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8041 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8101 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8161 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8221 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8281 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8341 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     8401 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc
     8461 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     8521 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     8581 ggtgtcgggg ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
     8641 tgcggtgtga aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc
     8701 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     8761 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggtttt
//
