LOCUS       sequence-258138-         630 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Maintenance of 2 plasmids with a single antibiotic in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Palanisamy N, Degen A, Morath A, Ballestin Ballestin J, Juraske C, 
            Ozturk MA, Sprenger GA, Youn JW, Schamel WW, Di Ventura B
  TITLE     Split intein-mediated selection of cells containing two plasmids 
            using a single antibiotic.
  JOURNAL   Nat Commun. 2019 Oct 31;10(1):4967. doi: 10.1038/s41467-019-12911-1.
  PUBMED    31672972
REFERENCE   2  (bases 1 to 630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-31"; volume: "10(1):4967. doi"; pages: " 
            10.1038/s41467-019-12911-1"
COMMENT     Sequence Label: pSiMPlk_N + pSiMPlk_C
FEATURES             Location/Qualifiers
     source          1..630
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(74..93)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 atgagccata ttcaacggga aacgtcttgc tctaggccgc gattaaattc caacatggat
       61 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
      121 tatcgattgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
      181 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
      241 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
      301 atccccggga aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaatctggt
      361 tactgcttgg atctgaaaac ccaggttcag accccgcagg gtatgaagga aatttccaac
      421 atccaggtcg gtgatctggt actgagcaac acgggttaca acgaagttct gaacgtcttc
      481 ccgaaatcta aaaaaaagtc ttacaaaatc accctggaag atggcaagga aatcatctgt
      541 tccgaagaac acctgtttcc gacgcagact ggtgaaatga acatctccgg tggtctgaaa
      601 gaaggtatgt gtctgtatgt taaagaataa
//
