LOCUS       sequence-258140-         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Maintenance of 2 plasmids with a single antibiotic in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Palanisamy N, Degen A, Morath A, Ballestin Ballestin J, Juraske C, 
            Ozturk MA, Sprenger GA, Youn JW, Schamel WW, Di Ventura B
  TITLE     Split intein-mediated selection of cells containing two plasmids 
            using a single antibiotic.
  JOURNAL   Nat Commun. 2019 Oct 31;10(1):4967. doi: 10.1038/s41467-019-12911-1.
  PUBMED    31672972
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-31"; volume: "10(1):4967. doi"; pages: " 
            10.1038/s41467-019-12911-1"
COMMENT     Sequence Label: pSiMPlc_N + pSiMPlc_C
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(67..86)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
       61 cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
      121 attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
      181 cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt
      241 gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
      301 acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
      361 tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
      421 tctggttact gcttggatct gaaaacccag gttcagaccc cgcagggtat gaaggaaatt
      481 tccaacatcc aggtcggtga tctggtactg agcaacacgg gttacaacga agttctgaac
      541 gtcttcccga aatctaaaaa aaagtcttac aaaatcaccc tggaagatgg caaggaaatc
      601 atctgttccg aagaacacct gtttccgacg cagactggtg aaatgaacat ctccggtggt
      661 ctgaaagaag gtatgtgtct gtatgttaaa gaataa
//
