LOCUS       sequence-258144-        1044 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Maintenance of 2 plasmids with a single antibiotic in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Palanisamy N, Degen A, Morath A, Ballestin Ballestin J, Juraske C, 
            Ozturk MA, Sprenger GA, Youn JW, Schamel WW, Di Ventura B
  TITLE     Split intein-mediated selection of cells containing two plasmids 
            using a single antibiotic.
  JOURNAL   Nat Commun. 2019 Oct 31;10(1):4967. doi: 10.1038/s41467-019-12911-1.
  PUBMED    31672972
REFERENCE   2  (bases 1 to 1044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-10-31"; volume: "10(1):4967. doi"; pages: " 
            10.1038/s41467-019-12911-1"
COMMENT     Sequence Label: pSiMPlh_N + pSiMPlh_C
FEATURES             Location/Qualifiers
     source          1..1044
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgggtaaaa agcctgaact caccgcgacg tctgtcgaga agtttctgat cgaaaagttc
       61 gacagcgtct ccgacctgat gcagctctcg gagggcgaag aatctcgtgc tttcagcttc
      121 gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa
      181 gatcgttatg tttatcggca ctttgcatcg gccgcgctcc cgattccgga agtgcttgac
      241 attggggagt ttagcgagag cctgacctat tgcatctccc gccgttcaca gggtgtcacg
      301 ttgcaagacc tgcctgaaac cgaactgccc gctgttctac aaccggtcgc ggaggctatg
      361 gatgcgatcg ctgcggccga tcttagccag acgagcgggt tcggcccatt cggaccgcaa
      421 ggaatcggtc aatacactac atggcgtgat ttcatatgcg cgattgctga tccccatgtg
      481 tatcactggc aaactgtgat ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat
      541 gagctgatgc tttgggccga ggactgcccc gaagtccggc acctcgtgca cgcggatttc
      601 ggctccaaca atgtcctgac ggacaatggc cgcataacag cggtcattga ctggagcgag
      661 gcgatgttcg gggattccca atacgaggtc gccaacatct tcttctggag gccgtggttg
      721 gcttgtatgg agcagcagac gcgctacttc gagcggaggc atcaggagtc tggttactgc
      781 ttggatctga aaacccaggt tcagaccccg cagggtatga aggaaatttc caacatccag
      841 gtcggtgatc tggtactgag caacacgggt tacaacgaag ttctgaacgt cttcccgaaa
      901 tctaaaaaaa agtcttacaa aatcaccctg gaagatggca aggaaatcat ctgttccgaa
      961 gaacacctgt ttccgacgca gactggtgaa atgaacatct ccggtggtct gaaagaaggt
     1021 atgtgtctgt atgttaaaga ataa
//
