LOCUS       sequence-25815-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Gong L, Kamitani T, Fujise K, Caskey LS, Yeh ET
  TITLE     Preferential interaction of sentrin with a ubiquitin-conjugating 
            enzyme, Ubc9.
  JOURNAL   J Biol Chem. 1997 Nov 7;272(45):28198-201.
  PUBMED    9353268
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1997-11-7"; volume: "272(45)"; pages: "28198-201"
COMMENT     Sequence Label: pcDNA3/UBC9
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(638..656)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(639..656)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(676..693)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    682..906
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctctctggct actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttggt accgagctcg gatccatgtc ggggatcgcc ctcagcagac
      121 tcgcccagga gaggaaagca tggaggaaag accacccatt tggtttcgtg gctgtcccaa
      181 caaaaaatcc cgatggcacg atgaacctca tgaactggga gtgcgccatt ccaggaaaga
      241 aagggactcc gtgggaagga ggcttgttta aactacggat gcttttcaaa gatgattatc
      301 catcttcgcc accaaaatgt aaattcgaac caccattatt tcacccgaat gtgtaccctt
      361 cggggacagt gtgcctgtcc atcttagagg aggacaagga ctggaggcca gccatcacaa
      421 tcaaacagat cctattagga atacaggaac ttctaaatga accaaatatc caagacccag
      481 ctcaagcaga ggcctacacg atttactgcc aaaacagagt ggagtacgag aaaagggtcc
      541 gagcacaagc caagaagttt gcgccctcat aagcagcgaa ttctgcagat atccatcaca
      601 ctggcggccg ctcgagcatg catctagagg gccctattct atagtgtcac ctaaatgcta
      661 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
      721 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
      781 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
      841 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
      901 ctatggcttc tgaggcggaa agaaccagct
//
